Cargando…
The Regulating Mechanism of Chrysophanol on Protein Level of CaM-CaMKIV to Protect PC12 Cells Against Aβ(25-35)-Induced Damage
OBJECTIVE: To investigate the neuroprotective effect of chrysophanol (CHR) on PC12 treated with Aβ(25-35), and the involved mechanism. METHODS: After the establishment of an AD cell model induced by Aβ(25-35), the cell survival rate was detected by MTT, cell apoptosis was assayed by Hoechst 33342 st...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7381772/ https://www.ncbi.nlm.nih.gov/pubmed/32764873 http://dx.doi.org/10.2147/DDDT.S245128 |
_version_ | 1783563114649747456 |
---|---|
author | Ye, Ting Gao, Hua-Wu Xuan, Wei-Ting Ye, Shu Zhou, Peng Li, Xin-Quan Wang, Yan Song, Hang Liu, Yan-Yan Cai, Biao |
author_facet | Ye, Ting Gao, Hua-Wu Xuan, Wei-Ting Ye, Shu Zhou, Peng Li, Xin-Quan Wang, Yan Song, Hang Liu, Yan-Yan Cai, Biao |
author_sort | Ye, Ting |
collection | PubMed |
description | OBJECTIVE: To investigate the neuroprotective effect of chrysophanol (CHR) on PC12 treated with Aβ(25-35), and the involved mechanism. METHODS: After the establishment of an AD cell model induced by Aβ(25-35), the cell survival rate was detected by MTT, cell apoptosis was assayed by Hoechst 33342 staining, mRNA expressions of calmodulin (CaM), calcium/calmodulin-dependent protein kinase kinase (CaMKK), calcium/calmodulin-dependent protein kinase IV (CaMKIV) and tau (MAPT; commonly known as tau) were determined by qRT-PCR, and protein levels of CaM, CaMKK, CaMKIV, phospho-CaMKIV (p-CaMKIV), tau and phospho-tau (p-tau) were detected by Western blot analysis. RESULTS: When pretreated with CHR before exposure to Aβ(25-35), PC12 cells showed that increased cell viability and reduced apoptosis. The qRT-PCR results indicated that the deposition of Aβ(25-35) triggers a decrease in levels of CaM, CaMKK, CaMKIV, and tau in PC12 cells. In addition, Western blot results also suggested that Aβ(25-35) decreases the protein expression of CaM, CaMKK, CaMKIV, p-CaMKIV, and the ratio of p-tau to tau in PC12 cells. However, the above effects were significantly alleviated after the treatment of CHR. CONCLUSION: CHR plays a neuroprotective role in AD though decreasing the protein level of CaM-CaMKK-CaMKIV and the expression of p-tau downstream. |
format | Online Article Text |
id | pubmed-7381772 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-73817722020-08-05 The Regulating Mechanism of Chrysophanol on Protein Level of CaM-CaMKIV to Protect PC12 Cells Against Aβ(25-35)-Induced Damage Ye, Ting Gao, Hua-Wu Xuan, Wei-Ting Ye, Shu Zhou, Peng Li, Xin-Quan Wang, Yan Song, Hang Liu, Yan-Yan Cai, Biao Drug Des Devel Ther Original Research OBJECTIVE: To investigate the neuroprotective effect of chrysophanol (CHR) on PC12 treated with Aβ(25-35), and the involved mechanism. METHODS: After the establishment of an AD cell model induced by Aβ(25-35), the cell survival rate was detected by MTT, cell apoptosis was assayed by Hoechst 33342 staining, mRNA expressions of calmodulin (CaM), calcium/calmodulin-dependent protein kinase kinase (CaMKK), calcium/calmodulin-dependent protein kinase IV (CaMKIV) and tau (MAPT; commonly known as tau) were determined by qRT-PCR, and protein levels of CaM, CaMKK, CaMKIV, phospho-CaMKIV (p-CaMKIV), tau and phospho-tau (p-tau) were detected by Western blot analysis. RESULTS: When pretreated with CHR before exposure to Aβ(25-35), PC12 cells showed that increased cell viability and reduced apoptosis. The qRT-PCR results indicated that the deposition of Aβ(25-35) triggers a decrease in levels of CaM, CaMKK, CaMKIV, and tau in PC12 cells. In addition, Western blot results also suggested that Aβ(25-35) decreases the protein expression of CaM, CaMKK, CaMKIV, p-CaMKIV, and the ratio of p-tau to tau in PC12 cells. However, the above effects were significantly alleviated after the treatment of CHR. CONCLUSION: CHR plays a neuroprotective role in AD though decreasing the protein level of CaM-CaMKK-CaMKIV and the expression of p-tau downstream. Dove 2020-07-13 /pmc/articles/PMC7381772/ /pubmed/32764873 http://dx.doi.org/10.2147/DDDT.S245128 Text en © 2020 Ye et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Ye, Ting Gao, Hua-Wu Xuan, Wei-Ting Ye, Shu Zhou, Peng Li, Xin-Quan Wang, Yan Song, Hang Liu, Yan-Yan Cai, Biao The Regulating Mechanism of Chrysophanol on Protein Level of CaM-CaMKIV to Protect PC12 Cells Against Aβ(25-35)-Induced Damage |
title | The Regulating Mechanism of Chrysophanol on Protein Level of CaM-CaMKIV to Protect PC12 Cells Against Aβ(25-35)-Induced Damage |
title_full | The Regulating Mechanism of Chrysophanol on Protein Level of CaM-CaMKIV to Protect PC12 Cells Against Aβ(25-35)-Induced Damage |
title_fullStr | The Regulating Mechanism of Chrysophanol on Protein Level of CaM-CaMKIV to Protect PC12 Cells Against Aβ(25-35)-Induced Damage |
title_full_unstemmed | The Regulating Mechanism of Chrysophanol on Protein Level of CaM-CaMKIV to Protect PC12 Cells Against Aβ(25-35)-Induced Damage |
title_short | The Regulating Mechanism of Chrysophanol on Protein Level of CaM-CaMKIV to Protect PC12 Cells Against Aβ(25-35)-Induced Damage |
title_sort | regulating mechanism of chrysophanol on protein level of cam-camkiv to protect pc12 cells against aβ(25-35)-induced damage |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7381772/ https://www.ncbi.nlm.nih.gov/pubmed/32764873 http://dx.doi.org/10.2147/DDDT.S245128 |
work_keys_str_mv | AT yeting theregulatingmechanismofchrysophanolonproteinlevelofcamcamkivtoprotectpc12cellsagainstab2535induceddamage AT gaohuawu theregulatingmechanismofchrysophanolonproteinlevelofcamcamkivtoprotectpc12cellsagainstab2535induceddamage AT xuanweiting theregulatingmechanismofchrysophanolonproteinlevelofcamcamkivtoprotectpc12cellsagainstab2535induceddamage AT yeshu theregulatingmechanismofchrysophanolonproteinlevelofcamcamkivtoprotectpc12cellsagainstab2535induceddamage AT zhoupeng theregulatingmechanismofchrysophanolonproteinlevelofcamcamkivtoprotectpc12cellsagainstab2535induceddamage AT lixinquan theregulatingmechanismofchrysophanolonproteinlevelofcamcamkivtoprotectpc12cellsagainstab2535induceddamage AT wangyan theregulatingmechanismofchrysophanolonproteinlevelofcamcamkivtoprotectpc12cellsagainstab2535induceddamage AT songhang theregulatingmechanismofchrysophanolonproteinlevelofcamcamkivtoprotectpc12cellsagainstab2535induceddamage AT liuyanyan theregulatingmechanismofchrysophanolonproteinlevelofcamcamkivtoprotectpc12cellsagainstab2535induceddamage AT caibiao theregulatingmechanismofchrysophanolonproteinlevelofcamcamkivtoprotectpc12cellsagainstab2535induceddamage AT yeting regulatingmechanismofchrysophanolonproteinlevelofcamcamkivtoprotectpc12cellsagainstab2535induceddamage AT gaohuawu regulatingmechanismofchrysophanolonproteinlevelofcamcamkivtoprotectpc12cellsagainstab2535induceddamage AT xuanweiting regulatingmechanismofchrysophanolonproteinlevelofcamcamkivtoprotectpc12cellsagainstab2535induceddamage AT yeshu regulatingmechanismofchrysophanolonproteinlevelofcamcamkivtoprotectpc12cellsagainstab2535induceddamage AT zhoupeng regulatingmechanismofchrysophanolonproteinlevelofcamcamkivtoprotectpc12cellsagainstab2535induceddamage AT lixinquan regulatingmechanismofchrysophanolonproteinlevelofcamcamkivtoprotectpc12cellsagainstab2535induceddamage AT wangyan regulatingmechanismofchrysophanolonproteinlevelofcamcamkivtoprotectpc12cellsagainstab2535induceddamage AT songhang regulatingmechanismofchrysophanolonproteinlevelofcamcamkivtoprotectpc12cellsagainstab2535induceddamage AT liuyanyan regulatingmechanismofchrysophanolonproteinlevelofcamcamkivtoprotectpc12cellsagainstab2535induceddamage AT caibiao regulatingmechanismofchrysophanolonproteinlevelofcamcamkivtoprotectpc12cellsagainstab2535induceddamage |