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The Regulating Mechanism of Chrysophanol on Protein Level of CaM-CaMKIV to Protect PC12 Cells Against Aβ(25-35)-Induced Damage

OBJECTIVE: To investigate the neuroprotective effect of chrysophanol (CHR) on PC12 treated with Aβ(25-35), and the involved mechanism. METHODS: After the establishment of an AD cell model induced by Aβ(25-35), the cell survival rate was detected by MTT, cell apoptosis was assayed by Hoechst 33342 st...

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Autores principales: Ye, Ting, Gao, Hua-Wu, Xuan, Wei-Ting, Ye, Shu, Zhou, Peng, Li, Xin-Quan, Wang, Yan, Song, Hang, Liu, Yan-Yan, Cai, Biao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7381772/
https://www.ncbi.nlm.nih.gov/pubmed/32764873
http://dx.doi.org/10.2147/DDDT.S245128
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author Ye, Ting
Gao, Hua-Wu
Xuan, Wei-Ting
Ye, Shu
Zhou, Peng
Li, Xin-Quan
Wang, Yan
Song, Hang
Liu, Yan-Yan
Cai, Biao
author_facet Ye, Ting
Gao, Hua-Wu
Xuan, Wei-Ting
Ye, Shu
Zhou, Peng
Li, Xin-Quan
Wang, Yan
Song, Hang
Liu, Yan-Yan
Cai, Biao
author_sort Ye, Ting
collection PubMed
description OBJECTIVE: To investigate the neuroprotective effect of chrysophanol (CHR) on PC12 treated with Aβ(25-35), and the involved mechanism. METHODS: After the establishment of an AD cell model induced by Aβ(25-35), the cell survival rate was detected by MTT, cell apoptosis was assayed by Hoechst 33342 staining, mRNA expressions of calmodulin (CaM), calcium/calmodulin-dependent protein kinase kinase (CaMKK), calcium/calmodulin-dependent protein kinase IV (CaMKIV) and tau (MAPT; commonly known as tau) were determined by qRT-PCR, and protein levels of CaM, CaMKK, CaMKIV, phospho-CaMKIV (p-CaMKIV), tau and phospho-tau (p-tau) were detected by Western blot analysis. RESULTS: When pretreated with CHR before exposure to Aβ(25-35), PC12 cells showed that increased cell viability and reduced apoptosis. The qRT-PCR results indicated that the deposition of Aβ(25-35) triggers a decrease in levels of CaM, CaMKK, CaMKIV, and tau in PC12 cells. In addition, Western blot results also suggested that Aβ(25-35) decreases the protein expression of CaM, CaMKK, CaMKIV, p-CaMKIV, and the ratio of p-tau to tau in PC12 cells. However, the above effects were significantly alleviated after the treatment of CHR. CONCLUSION: CHR plays a neuroprotective role in AD though decreasing the protein level of CaM-CaMKK-CaMKIV and the expression of p-tau downstream.
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spelling pubmed-73817722020-08-05 The Regulating Mechanism of Chrysophanol on Protein Level of CaM-CaMKIV to Protect PC12 Cells Against Aβ(25-35)-Induced Damage Ye, Ting Gao, Hua-Wu Xuan, Wei-Ting Ye, Shu Zhou, Peng Li, Xin-Quan Wang, Yan Song, Hang Liu, Yan-Yan Cai, Biao Drug Des Devel Ther Original Research OBJECTIVE: To investigate the neuroprotective effect of chrysophanol (CHR) on PC12 treated with Aβ(25-35), and the involved mechanism. METHODS: After the establishment of an AD cell model induced by Aβ(25-35), the cell survival rate was detected by MTT, cell apoptosis was assayed by Hoechst 33342 staining, mRNA expressions of calmodulin (CaM), calcium/calmodulin-dependent protein kinase kinase (CaMKK), calcium/calmodulin-dependent protein kinase IV (CaMKIV) and tau (MAPT; commonly known as tau) were determined by qRT-PCR, and protein levels of CaM, CaMKK, CaMKIV, phospho-CaMKIV (p-CaMKIV), tau and phospho-tau (p-tau) were detected by Western blot analysis. RESULTS: When pretreated with CHR before exposure to Aβ(25-35), PC12 cells showed that increased cell viability and reduced apoptosis. The qRT-PCR results indicated that the deposition of Aβ(25-35) triggers a decrease in levels of CaM, CaMKK, CaMKIV, and tau in PC12 cells. In addition, Western blot results also suggested that Aβ(25-35) decreases the protein expression of CaM, CaMKK, CaMKIV, p-CaMKIV, and the ratio of p-tau to tau in PC12 cells. However, the above effects were significantly alleviated after the treatment of CHR. CONCLUSION: CHR plays a neuroprotective role in AD though decreasing the protein level of CaM-CaMKK-CaMKIV and the expression of p-tau downstream. Dove 2020-07-13 /pmc/articles/PMC7381772/ /pubmed/32764873 http://dx.doi.org/10.2147/DDDT.S245128 Text en © 2020 Ye et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Ye, Ting
Gao, Hua-Wu
Xuan, Wei-Ting
Ye, Shu
Zhou, Peng
Li, Xin-Quan
Wang, Yan
Song, Hang
Liu, Yan-Yan
Cai, Biao
The Regulating Mechanism of Chrysophanol on Protein Level of CaM-CaMKIV to Protect PC12 Cells Against Aβ(25-35)-Induced Damage
title The Regulating Mechanism of Chrysophanol on Protein Level of CaM-CaMKIV to Protect PC12 Cells Against Aβ(25-35)-Induced Damage
title_full The Regulating Mechanism of Chrysophanol on Protein Level of CaM-CaMKIV to Protect PC12 Cells Against Aβ(25-35)-Induced Damage
title_fullStr The Regulating Mechanism of Chrysophanol on Protein Level of CaM-CaMKIV to Protect PC12 Cells Against Aβ(25-35)-Induced Damage
title_full_unstemmed The Regulating Mechanism of Chrysophanol on Protein Level of CaM-CaMKIV to Protect PC12 Cells Against Aβ(25-35)-Induced Damage
title_short The Regulating Mechanism of Chrysophanol on Protein Level of CaM-CaMKIV to Protect PC12 Cells Against Aβ(25-35)-Induced Damage
title_sort regulating mechanism of chrysophanol on protein level of cam-camkiv to protect pc12 cells against aβ(25-35)-induced damage
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7381772/
https://www.ncbi.nlm.nih.gov/pubmed/32764873
http://dx.doi.org/10.2147/DDDT.S245128
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