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The Effects of Calligonum Extract and Low-Intensity Ultrasound on Motility, Viability, and DNA Fragmentation of Human Frozen-Thawed Semen Samples
BACKGROUND: The study aimed to evaluate the impact of Calligonum extract and US radiation on sperm parameters of cryopreserved human semen samples. MATERIALS AND METHODS: In this experimental study, twenty-five semen specimens were obtained from healthy semen donors and incubated in human tubal flui...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royan Institute
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7382682/ https://www.ncbi.nlm.nih.gov/pubmed/32681619 http://dx.doi.org/10.22074/ijfs.2020.5896 |
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author | Ekrami, Hamid Movahedin, Mansoureh Koosha, Fereshteh Mazaheri, Zohreh Mokhtari-Dizaji, Manijhe |
author_facet | Ekrami, Hamid Movahedin, Mansoureh Koosha, Fereshteh Mazaheri, Zohreh Mokhtari-Dizaji, Manijhe |
author_sort | Ekrami, Hamid |
collection | PubMed |
description | BACKGROUND: The study aimed to evaluate the impact of Calligonum extract and US radiation on sperm parameters of cryopreserved human semen samples. MATERIALS AND METHODS: In this experimental study, twenty-five semen specimens were obtained from healthy semen donors and incubated in human tubal fluid (HTF) medium supplemented with 10% human serum albumin (HSA) for 45 minutes. Samples were treated with Calligonum extract (10 μg/ml) alone (CGM group) and US radiation (LIPUS- exposed group) alone or a combination of both treatments (CGM+LIPUS). The US group received US stimulation (in both continuous and pulsed wave modes) at a frequency of 1 MHZ and intensity of 200 mW/cm2 for 200 seconds. Sperm morphology was assessed by Diff-Quik staining. The DNA fragmentation was evaluated the Halo sperm kit. Sperm parameters was analyzed by a computer-assisted semen analysis system. Reactive oxygen species (ROS) was assessed by flow cytometry. RESULTS: The results showed that the treatment with Calligonum extract significantly (P<0.05) increased the progressive motility of spermatozoa in the CGM group as compared with the control group. The application of low-intensity US significantly (P<0.05) decreased the motility and viability of spermatozoa in the US group when compared with the control group. Our findings also indicated that the use of both low-intensity US in continuous mode and Calligonum extract slightly increased progressive motility; however, such an increase was not statistically significant. The rate of DNA fragmentation was considerably higher (P<0.05) in control and LIPUS-exposed groups than the other groups. CONCLUSION: Treatment of spermatozoa with Calligonum extract slightly improved the sperm parameters due to its antioxidant activity, on the other hand, according to our results, US radiation did not improve sperm parameters which may be due to interference with the motility of sperm, as well as its physical effects on spermatozoa. |
format | Online Article Text |
id | pubmed-7382682 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Royan Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-73826822020-07-29 The Effects of Calligonum Extract and Low-Intensity Ultrasound on Motility, Viability, and DNA Fragmentation of Human Frozen-Thawed Semen Samples Ekrami, Hamid Movahedin, Mansoureh Koosha, Fereshteh Mazaheri, Zohreh Mokhtari-Dizaji, Manijhe Int J Fertil Steril Original Article BACKGROUND: The study aimed to evaluate the impact of Calligonum extract and US radiation on sperm parameters of cryopreserved human semen samples. MATERIALS AND METHODS: In this experimental study, twenty-five semen specimens were obtained from healthy semen donors and incubated in human tubal fluid (HTF) medium supplemented with 10% human serum albumin (HSA) for 45 minutes. Samples were treated with Calligonum extract (10 μg/ml) alone (CGM group) and US radiation (LIPUS- exposed group) alone or a combination of both treatments (CGM+LIPUS). The US group received US stimulation (in both continuous and pulsed wave modes) at a frequency of 1 MHZ and intensity of 200 mW/cm2 for 200 seconds. Sperm morphology was assessed by Diff-Quik staining. The DNA fragmentation was evaluated the Halo sperm kit. Sperm parameters was analyzed by a computer-assisted semen analysis system. Reactive oxygen species (ROS) was assessed by flow cytometry. RESULTS: The results showed that the treatment with Calligonum extract significantly (P<0.05) increased the progressive motility of spermatozoa in the CGM group as compared with the control group. The application of low-intensity US significantly (P<0.05) decreased the motility and viability of spermatozoa in the US group when compared with the control group. Our findings also indicated that the use of both low-intensity US in continuous mode and Calligonum extract slightly increased progressive motility; however, such an increase was not statistically significant. The rate of DNA fragmentation was considerably higher (P<0.05) in control and LIPUS-exposed groups than the other groups. CONCLUSION: Treatment of spermatozoa with Calligonum extract slightly improved the sperm parameters due to its antioxidant activity, on the other hand, according to our results, US radiation did not improve sperm parameters which may be due to interference with the motility of sperm, as well as its physical effects on spermatozoa. Royan Institute 2020 2020-07-15 /pmc/articles/PMC7382682/ /pubmed/32681619 http://dx.doi.org/10.22074/ijfs.2020.5896 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Ekrami, Hamid Movahedin, Mansoureh Koosha, Fereshteh Mazaheri, Zohreh Mokhtari-Dizaji, Manijhe The Effects of Calligonum Extract and Low-Intensity Ultrasound on Motility, Viability, and DNA Fragmentation of Human Frozen-Thawed Semen Samples |
title | The Effects of Calligonum Extract and Low-Intensity Ultrasound
on Motility, Viability, and DNA Fragmentation of Human
Frozen-Thawed Semen Samples |
title_full | The Effects of Calligonum Extract and Low-Intensity Ultrasound
on Motility, Viability, and DNA Fragmentation of Human
Frozen-Thawed Semen Samples |
title_fullStr | The Effects of Calligonum Extract and Low-Intensity Ultrasound
on Motility, Viability, and DNA Fragmentation of Human
Frozen-Thawed Semen Samples |
title_full_unstemmed | The Effects of Calligonum Extract and Low-Intensity Ultrasound
on Motility, Viability, and DNA Fragmentation of Human
Frozen-Thawed Semen Samples |
title_short | The Effects of Calligonum Extract and Low-Intensity Ultrasound
on Motility, Viability, and DNA Fragmentation of Human
Frozen-Thawed Semen Samples |
title_sort | effects of calligonum extract and low-intensity ultrasound
on motility, viability, and dna fragmentation of human
frozen-thawed semen samples |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7382682/ https://www.ncbi.nlm.nih.gov/pubmed/32681619 http://dx.doi.org/10.22074/ijfs.2020.5896 |
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