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Curcumin Inhibits Hepatocellular Carcinoma via Regulating miR-21/TIMP3 Axis
BACKGROUND/AIM: Curcumin exhibits anticancer effects against various types of cancer including hepatocellular carcinoma (HCC). miR-21 has been reported to be involved in the malignant biological properties of HCC. However, whether miR-21 plays a role in curcumin-mediated treatment of HCC is unknown....
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7382716/ https://www.ncbi.nlm.nih.gov/pubmed/32724322 http://dx.doi.org/10.1155/2020/2892917 |
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author | Li, Jingtao Wei, Hailiang Liu, Yonggang Li, Qian Guo, Hui Guo, Yingjun Chang, Zhanjie |
author_facet | Li, Jingtao Wei, Hailiang Liu, Yonggang Li, Qian Guo, Hui Guo, Yingjun Chang, Zhanjie |
author_sort | Li, Jingtao |
collection | PubMed |
description | BACKGROUND/AIM: Curcumin exhibits anticancer effects against various types of cancer including hepatocellular carcinoma (HCC). miR-21 has been reported to be involved in the malignant biological properties of HCC. However, whether miR-21 plays a role in curcumin-mediated treatment of HCC is unknown. The purpose of this study was to identify the potential functions and mechanisms of miR-21 in curcumin-mediated treatment of HCC. METHODS: The anticancer effects of curcumin were assessed in vivo and in vitro. The underlying mechanism of miR-21 in curcumin-mediated treatment of HCC was assessed by quantitative real-time PCR (RT-qPCR), western blot, and Dual-Luciferase Reporter assays. RESULTS: The present study revealed that curcumin suppressed HCC growth in vivo and inhibited HCC cell proliferation and induced cell apoptosis in a dose-dependent manner in vitro. Meanwhile, the curcumin treatment can downregulate miR-21 expression, upregulate TIMP3 expression, and inhibit the TGF-β1/smad3 signaling pathway. miR-21 inhibition enhanced the effect of curcumin on cell proliferation inhibition, apoptosis, and TGF-β1/smad3 signaling pathway inhibition in HepG2 and HCCLM3 cells. It demonstrated that TIMP3 was a direct target gene of miR-21. Interestingly, the effect of miR-21 inhibition on cell proliferation, apoptosis, and TGF-β1/smad3 signaling pathway in HepG2 and HCCLM3 cells exposed to curcumin was attenuated by TIMP3 silencing. CONCLUSION: Taken together, the present study suggests that miR-21 is involved in the anticancer activities of curcumin through targeting TIMP3, and the mechanism possibly refers to the inhibition of TGF-β1/smad3 signaling pathway. |
format | Online Article Text |
id | pubmed-7382716 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-73827162020-07-27 Curcumin Inhibits Hepatocellular Carcinoma via Regulating miR-21/TIMP3 Axis Li, Jingtao Wei, Hailiang Liu, Yonggang Li, Qian Guo, Hui Guo, Yingjun Chang, Zhanjie Evid Based Complement Alternat Med Research Article BACKGROUND/AIM: Curcumin exhibits anticancer effects against various types of cancer including hepatocellular carcinoma (HCC). miR-21 has been reported to be involved in the malignant biological properties of HCC. However, whether miR-21 plays a role in curcumin-mediated treatment of HCC is unknown. The purpose of this study was to identify the potential functions and mechanisms of miR-21 in curcumin-mediated treatment of HCC. METHODS: The anticancer effects of curcumin were assessed in vivo and in vitro. The underlying mechanism of miR-21 in curcumin-mediated treatment of HCC was assessed by quantitative real-time PCR (RT-qPCR), western blot, and Dual-Luciferase Reporter assays. RESULTS: The present study revealed that curcumin suppressed HCC growth in vivo and inhibited HCC cell proliferation and induced cell apoptosis in a dose-dependent manner in vitro. Meanwhile, the curcumin treatment can downregulate miR-21 expression, upregulate TIMP3 expression, and inhibit the TGF-β1/smad3 signaling pathway. miR-21 inhibition enhanced the effect of curcumin on cell proliferation inhibition, apoptosis, and TGF-β1/smad3 signaling pathway inhibition in HepG2 and HCCLM3 cells. It demonstrated that TIMP3 was a direct target gene of miR-21. Interestingly, the effect of miR-21 inhibition on cell proliferation, apoptosis, and TGF-β1/smad3 signaling pathway in HepG2 and HCCLM3 cells exposed to curcumin was attenuated by TIMP3 silencing. CONCLUSION: Taken together, the present study suggests that miR-21 is involved in the anticancer activities of curcumin through targeting TIMP3, and the mechanism possibly refers to the inhibition of TGF-β1/smad3 signaling pathway. Hindawi 2020-07-17 /pmc/articles/PMC7382716/ /pubmed/32724322 http://dx.doi.org/10.1155/2020/2892917 Text en Copyright © 2020 Jingtao Li et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Li, Jingtao Wei, Hailiang Liu, Yonggang Li, Qian Guo, Hui Guo, Yingjun Chang, Zhanjie Curcumin Inhibits Hepatocellular Carcinoma via Regulating miR-21/TIMP3 Axis |
title | Curcumin Inhibits Hepatocellular Carcinoma via Regulating miR-21/TIMP3 Axis |
title_full | Curcumin Inhibits Hepatocellular Carcinoma via Regulating miR-21/TIMP3 Axis |
title_fullStr | Curcumin Inhibits Hepatocellular Carcinoma via Regulating miR-21/TIMP3 Axis |
title_full_unstemmed | Curcumin Inhibits Hepatocellular Carcinoma via Regulating miR-21/TIMP3 Axis |
title_short | Curcumin Inhibits Hepatocellular Carcinoma via Regulating miR-21/TIMP3 Axis |
title_sort | curcumin inhibits hepatocellular carcinoma via regulating mir-21/timp3 axis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7382716/ https://www.ncbi.nlm.nih.gov/pubmed/32724322 http://dx.doi.org/10.1155/2020/2892917 |
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