Polymerase chain reaction primer sets for the detection of genetically diverse human sapoviruses

Sapoviruses are increasingly being recognized as pathogens associated with gastroenteritis in humans. Human sapoviruses are currently assigned to 18 genotypes (GI.1-7, GII.1-8, GIV.1, and GV.1-2) based on the sequence of the region encoding the major structural protein. In this study, we evaluated 1...

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Autores principales: Oka, Tomoichiro, Yamamoto, Seiji P., Iritani, Nobuhiro, Sato, Shigenori, Tatsumi, Chika, Mita, Tetsuo, Yahiro, Shunsuke, Shibata, Shinichiro, Wu, Fang-Tzy, Takagi, Hirotaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2020
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7383071/
https://www.ncbi.nlm.nih.gov/pubmed/32719956
http://dx.doi.org/10.1007/s00705-020-04746-9
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author Oka, Tomoichiro
Yamamoto, Seiji P.
Iritani, Nobuhiro
Sato, Shigenori
Tatsumi, Chika
Mita, Tetsuo
Yahiro, Shunsuke
Shibata, Shinichiro
Wu, Fang-Tzy
Takagi, Hirotaka
author_facet Oka, Tomoichiro
Yamamoto, Seiji P.
Iritani, Nobuhiro
Sato, Shigenori
Tatsumi, Chika
Mita, Tetsuo
Yahiro, Shunsuke
Shibata, Shinichiro
Wu, Fang-Tzy
Takagi, Hirotaka
author_sort Oka, Tomoichiro
collection PubMed
description Sapoviruses are increasingly being recognized as pathogens associated with gastroenteritis in humans. Human sapoviruses are currently assigned to 18 genotypes (GI.1-7, GII.1-8, GIV.1, and GV.1-2) based on the sequence of the region encoding the major structural protein. In this study, we evaluated 11 polymerase chain reaction (PCR) assays using published and newly designed/modified primers and showed that four PCR assays with different primer combinations amplified all of the tested human sapovirus genotypes using either synthetic DNA or cDNA prepared from human sapovirus-positive fecal specimens. These assays can be used as improved broadly reactive screening tests or as tools for molecular characterization of human sapoviruses.
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spelling pubmed-73830712020-07-28 Polymerase chain reaction primer sets for the detection of genetically diverse human sapoviruses Oka, Tomoichiro Yamamoto, Seiji P. Iritani, Nobuhiro Sato, Shigenori Tatsumi, Chika Mita, Tetsuo Yahiro, Shunsuke Shibata, Shinichiro Wu, Fang-Tzy Takagi, Hirotaka Arch Virol Brief Report Sapoviruses are increasingly being recognized as pathogens associated with gastroenteritis in humans. Human sapoviruses are currently assigned to 18 genotypes (GI.1-7, GII.1-8, GIV.1, and GV.1-2) based on the sequence of the region encoding the major structural protein. In this study, we evaluated 11 polymerase chain reaction (PCR) assays using published and newly designed/modified primers and showed that four PCR assays with different primer combinations amplified all of the tested human sapovirus genotypes using either synthetic DNA or cDNA prepared from human sapovirus-positive fecal specimens. These assays can be used as improved broadly reactive screening tests or as tools for molecular characterization of human sapoviruses. Springer Vienna 2020-07-27 2020 /pmc/articles/PMC7383071/ /pubmed/32719956 http://dx.doi.org/10.1007/s00705-020-04746-9 Text en © Springer-Verlag GmbH Austria, part of Springer Nature 2020 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Oka, Tomoichiro
Yamamoto, Seiji P.
Iritani, Nobuhiro
Sato, Shigenori
Tatsumi, Chika
Mita, Tetsuo
Yahiro, Shunsuke
Shibata, Shinichiro
Wu, Fang-Tzy
Takagi, Hirotaka
Polymerase chain reaction primer sets for the detection of genetically diverse human sapoviruses
title Polymerase chain reaction primer sets for the detection of genetically diverse human sapoviruses
title_full Polymerase chain reaction primer sets for the detection of genetically diverse human sapoviruses
title_fullStr Polymerase chain reaction primer sets for the detection of genetically diverse human sapoviruses
title_full_unstemmed Polymerase chain reaction primer sets for the detection of genetically diverse human sapoviruses
title_short Polymerase chain reaction primer sets for the detection of genetically diverse human sapoviruses
title_sort polymerase chain reaction primer sets for the detection of genetically diverse human sapoviruses
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7383071/
https://www.ncbi.nlm.nih.gov/pubmed/32719956
http://dx.doi.org/10.1007/s00705-020-04746-9
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