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The Platelet microRNA Profile of Kawasaki Disease: Identification of Novel Diagnostic Biomarkers
Challenging diagnosis and unknown etiology of Kawasaki disease (KD) increase the coronary artery lesions incidence. microRNAs (miRNAs) are the most promising biomarkers because of their stability in peripheral blood and noninvasive measurement procedure, whose potential utility have been proved in c...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7383328/ https://www.ncbi.nlm.nih.gov/pubmed/32733962 http://dx.doi.org/10.1155/2020/9061568 |
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author | Ning, Qianqian Chen, Liqin Song, Sirui Zhang, Hong Xu, Kangping Liu, Jia Zhou, Yiwen Zang, Chenyang Li, Guang Chen, Feng Jia, Jia Ding, Guohui Huang, Min |
author_facet | Ning, Qianqian Chen, Liqin Song, Sirui Zhang, Hong Xu, Kangping Liu, Jia Zhou, Yiwen Zang, Chenyang Li, Guang Chen, Feng Jia, Jia Ding, Guohui Huang, Min |
author_sort | Ning, Qianqian |
collection | PubMed |
description | Challenging diagnosis and unknown etiology of Kawasaki disease (KD) increase the coronary artery lesions incidence. microRNAs (miRNAs) are the most promising biomarkers because of their stability in peripheral blood and noninvasive measurement procedure, whose potential utility have been proved in cancers. To explore the utility of differentially expressed (DE) miRNAs as early diagnostic markers, 44 patients (25 incomplete KD and 19 complete KD) and 31 febrile controls were recruited for small RNA sequencing. From all the 1922 expressed miRNA, 210 DE miRNAs were found between KD and febrile control groups. Though platelet miRNA profiles of complete KD incomplete KD were much similar through cluster analysis, the DE miRNAs were not identical. Eight DE miRNAs were validated by real-time quantitative PCR (qRT-PCR) in complete or incomplete KD groups using a normalizer, miR-126-3p, which was identified by geNorm and NormFinder tools. The expression level of miRNAs continuous changed over time was observed and the function analysis showed the potential role of miRNAs as therapeutic biomarkers. Additionally, the prediction model for KD showed a sensitivity of 78.8% and a specificity of 71.4%, respectively. This study used small RNA sequencing to identify miRNA biomarkers KD diagnosis based on a large sample size. Our findings shine a light on the understanding of molecular pathogenesis of KD and may improve the accuracy of KD diagnosis and prognosis in clinical. |
format | Online Article Text |
id | pubmed-7383328 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-73833282020-07-29 The Platelet microRNA Profile of Kawasaki Disease: Identification of Novel Diagnostic Biomarkers Ning, Qianqian Chen, Liqin Song, Sirui Zhang, Hong Xu, Kangping Liu, Jia Zhou, Yiwen Zang, Chenyang Li, Guang Chen, Feng Jia, Jia Ding, Guohui Huang, Min Biomed Res Int Research Article Challenging diagnosis and unknown etiology of Kawasaki disease (KD) increase the coronary artery lesions incidence. microRNAs (miRNAs) are the most promising biomarkers because of their stability in peripheral blood and noninvasive measurement procedure, whose potential utility have been proved in cancers. To explore the utility of differentially expressed (DE) miRNAs as early diagnostic markers, 44 patients (25 incomplete KD and 19 complete KD) and 31 febrile controls were recruited for small RNA sequencing. From all the 1922 expressed miRNA, 210 DE miRNAs were found between KD and febrile control groups. Though platelet miRNA profiles of complete KD incomplete KD were much similar through cluster analysis, the DE miRNAs were not identical. Eight DE miRNAs were validated by real-time quantitative PCR (qRT-PCR) in complete or incomplete KD groups using a normalizer, miR-126-3p, which was identified by geNorm and NormFinder tools. The expression level of miRNAs continuous changed over time was observed and the function analysis showed the potential role of miRNAs as therapeutic biomarkers. Additionally, the prediction model for KD showed a sensitivity of 78.8% and a specificity of 71.4%, respectively. This study used small RNA sequencing to identify miRNA biomarkers KD diagnosis based on a large sample size. Our findings shine a light on the understanding of molecular pathogenesis of KD and may improve the accuracy of KD diagnosis and prognosis in clinical. Hindawi 2020-07-17 /pmc/articles/PMC7383328/ /pubmed/32733962 http://dx.doi.org/10.1155/2020/9061568 Text en Copyright © 2020 Qianqian Ning et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ning, Qianqian Chen, Liqin Song, Sirui Zhang, Hong Xu, Kangping Liu, Jia Zhou, Yiwen Zang, Chenyang Li, Guang Chen, Feng Jia, Jia Ding, Guohui Huang, Min The Platelet microRNA Profile of Kawasaki Disease: Identification of Novel Diagnostic Biomarkers |
title | The Platelet microRNA Profile of Kawasaki Disease: Identification of Novel Diagnostic Biomarkers |
title_full | The Platelet microRNA Profile of Kawasaki Disease: Identification of Novel Diagnostic Biomarkers |
title_fullStr | The Platelet microRNA Profile of Kawasaki Disease: Identification of Novel Diagnostic Biomarkers |
title_full_unstemmed | The Platelet microRNA Profile of Kawasaki Disease: Identification of Novel Diagnostic Biomarkers |
title_short | The Platelet microRNA Profile of Kawasaki Disease: Identification of Novel Diagnostic Biomarkers |
title_sort | platelet microrna profile of kawasaki disease: identification of novel diagnostic biomarkers |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7383328/ https://www.ncbi.nlm.nih.gov/pubmed/32733962 http://dx.doi.org/10.1155/2020/9061568 |
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