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Sensor Array Based Determination of Edman Degradated Amino Acids Using Poly(p‐phenyleneethynylene)s

A cross‐reactive optical sensor array based on poly(p‐phenyleneethynylene)s (PPEs) determines Edman degraded amino acids. We report a sensor array composed of three anionic PPEs P1–P3, and their electrostatic complexes with metal ions (Fe(2+), Cu(2+), Co(2+)). We recorded distinct fluorescence inten...

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Detalles Bibliográficos
Autores principales: Zhang, Hao, Wang, Benhua, Seehafer, Kai, Bunz, Uwe H. F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7383564/
https://www.ncbi.nlm.nih.gov/pubmed/32181541
http://dx.doi.org/10.1002/chem.202001262
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author Zhang, Hao
Wang, Benhua
Seehafer, Kai
Bunz, Uwe H. F.
author_facet Zhang, Hao
Wang, Benhua
Seehafer, Kai
Bunz, Uwe H. F.
author_sort Zhang, Hao
collection PubMed
description A cross‐reactive optical sensor array based on poly(p‐phenyleneethynylene)s (PPEs) determines Edman degraded amino acids. We report a sensor array composed of three anionic PPEs P1–P3, and their electrostatic complexes with metal ions (Fe(2+), Cu(2+), Co(2+)). We recorded distinct fluorescence intensity response patterns as “fingerprints” of this chemical tongue toward standard phenylthiohydantoin (PTH) amino acids—degradation products of the Edman process. These “fingerprints” were converted into canonical scores by linear discrimination analysis (LDA), which differentiates all of the PTH‐amino acids. This array discriminates PTH‐amino acid residues degraded from an oligopeptide through Edman sequencing. This approach is complementary to chromatography approaches which rely on mass spectrometry; our array offers the advantage of simplicity.
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spelling pubmed-73835642020-07-27 Sensor Array Based Determination of Edman Degradated Amino Acids Using Poly(p‐phenyleneethynylene)s Zhang, Hao Wang, Benhua Seehafer, Kai Bunz, Uwe H. F. Chemistry Communications A cross‐reactive optical sensor array based on poly(p‐phenyleneethynylene)s (PPEs) determines Edman degraded amino acids. We report a sensor array composed of three anionic PPEs P1–P3, and their electrostatic complexes with metal ions (Fe(2+), Cu(2+), Co(2+)). We recorded distinct fluorescence intensity response patterns as “fingerprints” of this chemical tongue toward standard phenylthiohydantoin (PTH) amino acids—degradation products of the Edman process. These “fingerprints” were converted into canonical scores by linear discrimination analysis (LDA), which differentiates all of the PTH‐amino acids. This array discriminates PTH‐amino acid residues degraded from an oligopeptide through Edman sequencing. This approach is complementary to chromatography approaches which rely on mass spectrometry; our array offers the advantage of simplicity. John Wiley and Sons Inc. 2020-05-29 2020-06-23 /pmc/articles/PMC7383564/ /pubmed/32181541 http://dx.doi.org/10.1002/chem.202001262 Text en © 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Communications
Zhang, Hao
Wang, Benhua
Seehafer, Kai
Bunz, Uwe H. F.
Sensor Array Based Determination of Edman Degradated Amino Acids Using Poly(p‐phenyleneethynylene)s
title Sensor Array Based Determination of Edman Degradated Amino Acids Using Poly(p‐phenyleneethynylene)s
title_full Sensor Array Based Determination of Edman Degradated Amino Acids Using Poly(p‐phenyleneethynylene)s
title_fullStr Sensor Array Based Determination of Edman Degradated Amino Acids Using Poly(p‐phenyleneethynylene)s
title_full_unstemmed Sensor Array Based Determination of Edman Degradated Amino Acids Using Poly(p‐phenyleneethynylene)s
title_short Sensor Array Based Determination of Edman Degradated Amino Acids Using Poly(p‐phenyleneethynylene)s
title_sort sensor array based determination of edman degradated amino acids using poly(p‐phenyleneethynylene)s
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7383564/
https://www.ncbi.nlm.nih.gov/pubmed/32181541
http://dx.doi.org/10.1002/chem.202001262
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