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Sensor Array Based Determination of Edman Degradated Amino Acids Using Poly(p‐phenyleneethynylene)s
A cross‐reactive optical sensor array based on poly(p‐phenyleneethynylene)s (PPEs) determines Edman degraded amino acids. We report a sensor array composed of three anionic PPEs P1–P3, and their electrostatic complexes with metal ions (Fe(2+), Cu(2+), Co(2+)). We recorded distinct fluorescence inten...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7383564/ https://www.ncbi.nlm.nih.gov/pubmed/32181541 http://dx.doi.org/10.1002/chem.202001262 |
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author | Zhang, Hao Wang, Benhua Seehafer, Kai Bunz, Uwe H. F. |
author_facet | Zhang, Hao Wang, Benhua Seehafer, Kai Bunz, Uwe H. F. |
author_sort | Zhang, Hao |
collection | PubMed |
description | A cross‐reactive optical sensor array based on poly(p‐phenyleneethynylene)s (PPEs) determines Edman degraded amino acids. We report a sensor array composed of three anionic PPEs P1–P3, and their electrostatic complexes with metal ions (Fe(2+), Cu(2+), Co(2+)). We recorded distinct fluorescence intensity response patterns as “fingerprints” of this chemical tongue toward standard phenylthiohydantoin (PTH) amino acids—degradation products of the Edman process. These “fingerprints” were converted into canonical scores by linear discrimination analysis (LDA), which differentiates all of the PTH‐amino acids. This array discriminates PTH‐amino acid residues degraded from an oligopeptide through Edman sequencing. This approach is complementary to chromatography approaches which rely on mass spectrometry; our array offers the advantage of simplicity. |
format | Online Article Text |
id | pubmed-7383564 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73835642020-07-27 Sensor Array Based Determination of Edman Degradated Amino Acids Using Poly(p‐phenyleneethynylene)s Zhang, Hao Wang, Benhua Seehafer, Kai Bunz, Uwe H. F. Chemistry Communications A cross‐reactive optical sensor array based on poly(p‐phenyleneethynylene)s (PPEs) determines Edman degraded amino acids. We report a sensor array composed of three anionic PPEs P1–P3, and their electrostatic complexes with metal ions (Fe(2+), Cu(2+), Co(2+)). We recorded distinct fluorescence intensity response patterns as “fingerprints” of this chemical tongue toward standard phenylthiohydantoin (PTH) amino acids—degradation products of the Edman process. These “fingerprints” were converted into canonical scores by linear discrimination analysis (LDA), which differentiates all of the PTH‐amino acids. This array discriminates PTH‐amino acid residues degraded from an oligopeptide through Edman sequencing. This approach is complementary to chromatography approaches which rely on mass spectrometry; our array offers the advantage of simplicity. John Wiley and Sons Inc. 2020-05-29 2020-06-23 /pmc/articles/PMC7383564/ /pubmed/32181541 http://dx.doi.org/10.1002/chem.202001262 Text en © 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Communications Zhang, Hao Wang, Benhua Seehafer, Kai Bunz, Uwe H. F. Sensor Array Based Determination of Edman Degradated Amino Acids Using Poly(p‐phenyleneethynylene)s |
title | Sensor Array Based Determination of Edman Degradated Amino Acids Using Poly(p‐phenyleneethynylene)s |
title_full | Sensor Array Based Determination of Edman Degradated Amino Acids Using Poly(p‐phenyleneethynylene)s |
title_fullStr | Sensor Array Based Determination of Edman Degradated Amino Acids Using Poly(p‐phenyleneethynylene)s |
title_full_unstemmed | Sensor Array Based Determination of Edman Degradated Amino Acids Using Poly(p‐phenyleneethynylene)s |
title_short | Sensor Array Based Determination of Edman Degradated Amino Acids Using Poly(p‐phenyleneethynylene)s |
title_sort | sensor array based determination of edman degradated amino acids using poly(p‐phenyleneethynylene)s |
topic | Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7383564/ https://www.ncbi.nlm.nih.gov/pubmed/32181541 http://dx.doi.org/10.1002/chem.202001262 |
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