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In vitro biocompatibility of biohybrid polymers membrane evaluated in human gingival fibroblasts

The biohybrid polymer membrane (BHM) is a new biomaterial designed for the treatment of soft periodontal tissue defects. We aimed to evaluate the in vitro biocompatibility of the membrane in human gingival fibroblasts and the capability to induce cell adhesion, migration, differentiation and improvi...

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Autores principales: Guo, Bin, Tang, Chuhua, Wang, Mingguo, Zhao, Zhongqi, Shokoohi‐Tabrizi, Hassan A., Shi, Bin, Andrukhov, Oleh, Rausch‐Fan, Xiaohui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7383566/
https://www.ncbi.nlm.nih.gov/pubmed/32096606
http://dx.doi.org/10.1002/jbm.b.34591
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author Guo, Bin
Tang, Chuhua
Wang, Mingguo
Zhao, Zhongqi
Shokoohi‐Tabrizi, Hassan A.
Shi, Bin
Andrukhov, Oleh
Rausch‐Fan, Xiaohui
author_facet Guo, Bin
Tang, Chuhua
Wang, Mingguo
Zhao, Zhongqi
Shokoohi‐Tabrizi, Hassan A.
Shi, Bin
Andrukhov, Oleh
Rausch‐Fan, Xiaohui
author_sort Guo, Bin
collection PubMed
description The biohybrid polymer membrane (BHM) is a new biomaterial designed for the treatment of soft periodontal tissue defects. We aimed to evaluate the in vitro biocompatibility of the membrane in human gingival fibroblasts and the capability to induce cell adhesion, migration, differentiation and improving the production of the extracellular matrix. BHM and Mucograft® collagen matrix (MCM) membranes were punched into 6 mm diameter round discs and placed in 96‐well plates. Human primary gingival fibroblasts were seeded on the membranes or tissue culture plastic (TCP) serving as the control. Cell proliferation/viability and morphology were evaluated after 3, 7, and 14 days of culture by cell counting kit (CCK)‐8 assay and scanning electron microscopy, respectively. Additionally, the gene expression of transforming growth factor (TGF)‐β1, focal adhesion kinase (FAK), collagen type 1 (Col1), alpha‐smooth muscle actin (α‐SMA), and fibroblasts growth factor (FGF)‐2 was analyzed at 3, 7, and 14 days of culture by qPCR. Cell proliferation on BHM was significantly higher than on MCM and similar to TCP. Gene expression of TGF‐β1, FAK, Col1, and α‐SMA were significantly increased on BHM compared to TCP at most investigated time points. However, the gene expression of FGF‐2 was significantly decreased on BHM at Day 7 and recovered at Day 14 to the levels similar to TCP. The finding of this study showed that BHM is superior for gingival fibroblasts in terms of adhesion, proliferation, and gene expression, suggesting that this membrane may promote the healing of soft periodontal tissue.
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spelling pubmed-73835662020-07-27 In vitro biocompatibility of biohybrid polymers membrane evaluated in human gingival fibroblasts Guo, Bin Tang, Chuhua Wang, Mingguo Zhao, Zhongqi Shokoohi‐Tabrizi, Hassan A. Shi, Bin Andrukhov, Oleh Rausch‐Fan, Xiaohui J Biomed Mater Res B Appl Biomater Original Research Reports The biohybrid polymer membrane (BHM) is a new biomaterial designed for the treatment of soft periodontal tissue defects. We aimed to evaluate the in vitro biocompatibility of the membrane in human gingival fibroblasts and the capability to induce cell adhesion, migration, differentiation and improving the production of the extracellular matrix. BHM and Mucograft® collagen matrix (MCM) membranes were punched into 6 mm diameter round discs and placed in 96‐well plates. Human primary gingival fibroblasts were seeded on the membranes or tissue culture plastic (TCP) serving as the control. Cell proliferation/viability and morphology were evaluated after 3, 7, and 14 days of culture by cell counting kit (CCK)‐8 assay and scanning electron microscopy, respectively. Additionally, the gene expression of transforming growth factor (TGF)‐β1, focal adhesion kinase (FAK), collagen type 1 (Col1), alpha‐smooth muscle actin (α‐SMA), and fibroblasts growth factor (FGF)‐2 was analyzed at 3, 7, and 14 days of culture by qPCR. Cell proliferation on BHM was significantly higher than on MCM and similar to TCP. Gene expression of TGF‐β1, FAK, Col1, and α‐SMA were significantly increased on BHM compared to TCP at most investigated time points. However, the gene expression of FGF‐2 was significantly decreased on BHM at Day 7 and recovered at Day 14 to the levels similar to TCP. The finding of this study showed that BHM is superior for gingival fibroblasts in terms of adhesion, proliferation, and gene expression, suggesting that this membrane may promote the healing of soft periodontal tissue. John Wiley & Sons, Inc. 2020-02-25 2020-08 /pmc/articles/PMC7383566/ /pubmed/32096606 http://dx.doi.org/10.1002/jbm.b.34591 Text en © 2020 The Authors. Journal of Biomedical Materials Research Part B: Applied Biomaterials published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research Reports
Guo, Bin
Tang, Chuhua
Wang, Mingguo
Zhao, Zhongqi
Shokoohi‐Tabrizi, Hassan A.
Shi, Bin
Andrukhov, Oleh
Rausch‐Fan, Xiaohui
In vitro biocompatibility of biohybrid polymers membrane evaluated in human gingival fibroblasts
title In vitro biocompatibility of biohybrid polymers membrane evaluated in human gingival fibroblasts
title_full In vitro biocompatibility of biohybrid polymers membrane evaluated in human gingival fibroblasts
title_fullStr In vitro biocompatibility of biohybrid polymers membrane evaluated in human gingival fibroblasts
title_full_unstemmed In vitro biocompatibility of biohybrid polymers membrane evaluated in human gingival fibroblasts
title_short In vitro biocompatibility of biohybrid polymers membrane evaluated in human gingival fibroblasts
title_sort in vitro biocompatibility of biohybrid polymers membrane evaluated in human gingival fibroblasts
topic Original Research Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7383566/
https://www.ncbi.nlm.nih.gov/pubmed/32096606
http://dx.doi.org/10.1002/jbm.b.34591
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