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An immune response to the avascular lens following wounding of the cornea involves ciliary zonule fibrils

The lens and central cornea are avascular. It was assumed that the adult lens had no source of immune cells and that the basement membrane capsule surrounding the lens was a barrier to immune cell migration. Yet, microfibril‐associated protein‐1 (MAGP1)‐rich ciliary zonules that originate from the v...

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Autores principales: DeDreu, JodiRae, Bowen, Caitlin J., Logan, Caitlin M., Pal‐Ghosh, Sonali, Parlanti, Paola, Stepp, Mary Ann, Menko, A. Sue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7384020/
https://www.ncbi.nlm.nih.gov/pubmed/32452112
http://dx.doi.org/10.1096/fj.202000289R
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author DeDreu, JodiRae
Bowen, Caitlin J.
Logan, Caitlin M.
Pal‐Ghosh, Sonali
Parlanti, Paola
Stepp, Mary Ann
Menko, A. Sue
author_facet DeDreu, JodiRae
Bowen, Caitlin J.
Logan, Caitlin M.
Pal‐Ghosh, Sonali
Parlanti, Paola
Stepp, Mary Ann
Menko, A. Sue
author_sort DeDreu, JodiRae
collection PubMed
description The lens and central cornea are avascular. It was assumed that the adult lens had no source of immune cells and that the basement membrane capsule surrounding the lens was a barrier to immune cell migration. Yet, microfibril‐associated protein‐1 (MAGP1)‐rich ciliary zonules that originate from the vasculature‐rich ciliary body and extend along the surface of the lens capsule, form a potential conduit for immune cells to the lens. In response to cornea debridement wounding, we find increased expression of MAGP1 throughout the central corneal stroma. The immune cells that populate this typically avascular region after wounding closely associate with this MAGP1‐rich matrix. These results suggest that MAGP1‐rich microfibrils support immune cell migration post‐injury. Using this cornea wound model, we investigated whether there is an immune response to the lens following cornea injury involving the lens‐associated MAGP1‐rich ciliary zonules. Our results provide the first evidence that following corneal wounding immune cells are activated to travel along zonule fibers that extend anteriorly along the equatorial surface of the lens, from where they migrate across the anterior lens capsule. These results demonstrate that lens‐associated ciliary zonules are directly involved in the lens immune response and suggest the ciliary body as a source of immune cells to the avascular lens.
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spelling pubmed-73840202020-07-28 An immune response to the avascular lens following wounding of the cornea involves ciliary zonule fibrils DeDreu, JodiRae Bowen, Caitlin J. Logan, Caitlin M. Pal‐Ghosh, Sonali Parlanti, Paola Stepp, Mary Ann Menko, A. Sue FASEB J Research Articles The lens and central cornea are avascular. It was assumed that the adult lens had no source of immune cells and that the basement membrane capsule surrounding the lens was a barrier to immune cell migration. Yet, microfibril‐associated protein‐1 (MAGP1)‐rich ciliary zonules that originate from the vasculature‐rich ciliary body and extend along the surface of the lens capsule, form a potential conduit for immune cells to the lens. In response to cornea debridement wounding, we find increased expression of MAGP1 throughout the central corneal stroma. The immune cells that populate this typically avascular region after wounding closely associate with this MAGP1‐rich matrix. These results suggest that MAGP1‐rich microfibrils support immune cell migration post‐injury. Using this cornea wound model, we investigated whether there is an immune response to the lens following cornea injury involving the lens‐associated MAGP1‐rich ciliary zonules. Our results provide the first evidence that following corneal wounding immune cells are activated to travel along zonule fibers that extend anteriorly along the equatorial surface of the lens, from where they migrate across the anterior lens capsule. These results demonstrate that lens‐associated ciliary zonules are directly involved in the lens immune response and suggest the ciliary body as a source of immune cells to the avascular lens. John Wiley and Sons Inc. 2020-05-25 2020-07 /pmc/articles/PMC7384020/ /pubmed/32452112 http://dx.doi.org/10.1096/fj.202000289R Text en © 2020 The Authors. The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Research Articles
DeDreu, JodiRae
Bowen, Caitlin J.
Logan, Caitlin M.
Pal‐Ghosh, Sonali
Parlanti, Paola
Stepp, Mary Ann
Menko, A. Sue
An immune response to the avascular lens following wounding of the cornea involves ciliary zonule fibrils
title An immune response to the avascular lens following wounding of the cornea involves ciliary zonule fibrils
title_full An immune response to the avascular lens following wounding of the cornea involves ciliary zonule fibrils
title_fullStr An immune response to the avascular lens following wounding of the cornea involves ciliary zonule fibrils
title_full_unstemmed An immune response to the avascular lens following wounding of the cornea involves ciliary zonule fibrils
title_short An immune response to the avascular lens following wounding of the cornea involves ciliary zonule fibrils
title_sort immune response to the avascular lens following wounding of the cornea involves ciliary zonule fibrils
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7384020/
https://www.ncbi.nlm.nih.gov/pubmed/32452112
http://dx.doi.org/10.1096/fj.202000289R
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