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Identification and classification of epithelial cells in nephron segments by actin cytoskeleton patterns

The basic functional unit in a kidney is the nephron, which is a long and morphologically segmented tubule. The nephron begins with a cluster of capillaries called glomerulus through which the blood is filtered into the Bowman's space. The filtrate flows through the nephron segments. During thi...

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Autores principales: Kumaran, Girishkumar Kaitholil, Hanukoglu, Israel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7384063/
https://www.ncbi.nlm.nih.gov/pubmed/31605441
http://dx.doi.org/10.1111/febs.15088
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author Kumaran, Girishkumar Kaitholil
Hanukoglu, Israel
author_facet Kumaran, Girishkumar Kaitholil
Hanukoglu, Israel
author_sort Kumaran, Girishkumar Kaitholil
collection PubMed
description The basic functional unit in a kidney is the nephron, which is a long and morphologically segmented tubule. The nephron begins with a cluster of capillaries called glomerulus through which the blood is filtered into the Bowman's space. The filtrate flows through the nephron segments. During this flow, electrolytes and solutes are reabsorbed by channels and transport systems into the capillaries wrapped around the nephron. Many questions related to renal function focus on identifying the sites of expression of these systems. In this study, we mapped whole kidney sections by confocal microscopic imaging of fluorescent phalloidin, which binds to actin filaments. In tile scans (composed of hundreds of images) of these sections, the cortex and the medullary regions (outer and inner stripes of the outer medulla, and inner medulla) could be easily identified by their cytoskeletal patterns. At a higher resolution, we identified distinct features of the actin cytoskeleton in the apical, basal, and lateral borders of the cells. These features could be used to identify segments of a nephron (the proximal tubule, thin and thick segments of Henle's loop, and distal tubule), the collecting duct system, the papillary ducts in the papilla, and the urothelium that covers the pelvis. To verify our findings, we used additional markers, including aquaporin isoforms, cytokeratin 8‐18, and WGA lectin. This study highlights the power of high‐resolution confocal microscopy for identifying specific cell types using the simple probe of F‐actin‐binding phalloidin.
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spelling pubmed-73840632020-07-28 Identification and classification of epithelial cells in nephron segments by actin cytoskeleton patterns Kumaran, Girishkumar Kaitholil Hanukoglu, Israel FEBS J Original Articles The basic functional unit in a kidney is the nephron, which is a long and morphologically segmented tubule. The nephron begins with a cluster of capillaries called glomerulus through which the blood is filtered into the Bowman's space. The filtrate flows through the nephron segments. During this flow, electrolytes and solutes are reabsorbed by channels and transport systems into the capillaries wrapped around the nephron. Many questions related to renal function focus on identifying the sites of expression of these systems. In this study, we mapped whole kidney sections by confocal microscopic imaging of fluorescent phalloidin, which binds to actin filaments. In tile scans (composed of hundreds of images) of these sections, the cortex and the medullary regions (outer and inner stripes of the outer medulla, and inner medulla) could be easily identified by their cytoskeletal patterns. At a higher resolution, we identified distinct features of the actin cytoskeleton in the apical, basal, and lateral borders of the cells. These features could be used to identify segments of a nephron (the proximal tubule, thin and thick segments of Henle's loop, and distal tubule), the collecting duct system, the papillary ducts in the papilla, and the urothelium that covers the pelvis. To verify our findings, we used additional markers, including aquaporin isoforms, cytokeratin 8‐18, and WGA lectin. This study highlights the power of high‐resolution confocal microscopy for identifying specific cell types using the simple probe of F‐actin‐binding phalloidin. John Wiley and Sons Inc. 2019-11-01 2020-03 /pmc/articles/PMC7384063/ /pubmed/31605441 http://dx.doi.org/10.1111/febs.15088 Text en © 2019 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Kumaran, Girishkumar Kaitholil
Hanukoglu, Israel
Identification and classification of epithelial cells in nephron segments by actin cytoskeleton patterns
title Identification and classification of epithelial cells in nephron segments by actin cytoskeleton patterns
title_full Identification and classification of epithelial cells in nephron segments by actin cytoskeleton patterns
title_fullStr Identification and classification of epithelial cells in nephron segments by actin cytoskeleton patterns
title_full_unstemmed Identification and classification of epithelial cells in nephron segments by actin cytoskeleton patterns
title_short Identification and classification of epithelial cells in nephron segments by actin cytoskeleton patterns
title_sort identification and classification of epithelial cells in nephron segments by actin cytoskeleton patterns
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7384063/
https://www.ncbi.nlm.nih.gov/pubmed/31605441
http://dx.doi.org/10.1111/febs.15088
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