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Activating transcription factor 3 inhibits endometrial carcinoma aggressiveness via JunB suppression

The function of activating transcription factor 3 (ATF3) in cancer is context-dependent and its role in endometrial carcinoma (EC) is yet to be elucidated. In the present study, ATF3 was indicated to be downregulated, while one of the ATF3-interacting proteins, JunB, was upregulated in ECs according...

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Autores principales: Wang, Fangyuan, Li, Jingjing, Wang, Haixia, Zhang, Fan, Gao, Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7384851/
https://www.ncbi.nlm.nih.gov/pubmed/32582999
http://dx.doi.org/10.3892/ijo.2020.5084
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author Wang, Fangyuan
Li, Jingjing
Wang, Haixia
Zhang, Fan
Gao, Jin
author_facet Wang, Fangyuan
Li, Jingjing
Wang, Haixia
Zhang, Fan
Gao, Jin
author_sort Wang, Fangyuan
collection PubMed
description The function of activating transcription factor 3 (ATF3) in cancer is context-dependent and its role in endometrial carcinoma (EC) is yet to be elucidated. In the present study, ATF3 was indicated to be downregulated, while one of the ATF3-interacting proteins, JunB, was upregulated in ECs according to western blot analysis. After overexpression in ECs, ATF3 inhibited the proliferation and invasion of EC cells and enhanced apoptosis, as well as suppressed the expression of JunB. The properties of EC cells, including the expression of matrix metalloproteinases, tissue inhibitors of metallo-proteinases, the cell cycle and apoptosis were all altered by overexpression of ATF3. Furthermore, luciferase activity assay, chromatin precipitation and DNA affinity assay results indicated that ATF3 exerted the aforementioned functions via JunB binding and activator protein-1 signaling. However, the interaction between ATF3 and JunB did not occur in EC cells under basal conditions, but in ATF3-overexpressing ECs, which was capable of mitigating EC proliferation, invasion and metastasis. Collectively, the present results suggested that the ATF3/JunB interaction may serve as a potential therapeutic target for ECs.
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spelling pubmed-73848512020-07-30 Activating transcription factor 3 inhibits endometrial carcinoma aggressiveness via JunB suppression Wang, Fangyuan Li, Jingjing Wang, Haixia Zhang, Fan Gao, Jin Int J Oncol Articles The function of activating transcription factor 3 (ATF3) in cancer is context-dependent and its role in endometrial carcinoma (EC) is yet to be elucidated. In the present study, ATF3 was indicated to be downregulated, while one of the ATF3-interacting proteins, JunB, was upregulated in ECs according to western blot analysis. After overexpression in ECs, ATF3 inhibited the proliferation and invasion of EC cells and enhanced apoptosis, as well as suppressed the expression of JunB. The properties of EC cells, including the expression of matrix metalloproteinases, tissue inhibitors of metallo-proteinases, the cell cycle and apoptosis were all altered by overexpression of ATF3. Furthermore, luciferase activity assay, chromatin precipitation and DNA affinity assay results indicated that ATF3 exerted the aforementioned functions via JunB binding and activator protein-1 signaling. However, the interaction between ATF3 and JunB did not occur in EC cells under basal conditions, but in ATF3-overexpressing ECs, which was capable of mitigating EC proliferation, invasion and metastasis. Collectively, the present results suggested that the ATF3/JunB interaction may serve as a potential therapeutic target for ECs. D.A. Spandidos 2020-06-19 /pmc/articles/PMC7384851/ /pubmed/32582999 http://dx.doi.org/10.3892/ijo.2020.5084 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Wang, Fangyuan
Li, Jingjing
Wang, Haixia
Zhang, Fan
Gao, Jin
Activating transcription factor 3 inhibits endometrial carcinoma aggressiveness via JunB suppression
title Activating transcription factor 3 inhibits endometrial carcinoma aggressiveness via JunB suppression
title_full Activating transcription factor 3 inhibits endometrial carcinoma aggressiveness via JunB suppression
title_fullStr Activating transcription factor 3 inhibits endometrial carcinoma aggressiveness via JunB suppression
title_full_unstemmed Activating transcription factor 3 inhibits endometrial carcinoma aggressiveness via JunB suppression
title_short Activating transcription factor 3 inhibits endometrial carcinoma aggressiveness via JunB suppression
title_sort activating transcription factor 3 inhibits endometrial carcinoma aggressiveness via junb suppression
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7384851/
https://www.ncbi.nlm.nih.gov/pubmed/32582999
http://dx.doi.org/10.3892/ijo.2020.5084
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