Three-dimensional super-resolution fluorescence imaging of DNA

Recent advances in fluorescence super-resolution microscopy are providing important insights into details of cellular structures. To acquire three dimensional (3D) super-resolution images of DNA, we combined binding activated localization microscopy (BALM) using fluorescent double-stranded DNA inter...

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Detalles Bibliográficos
Autores principales: Yardimci, Sevim, Burnham, Daniel R., Terry, Samantha Y. A., Yardimci, Hasan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7385144/
https://www.ncbi.nlm.nih.gov/pubmed/32719468
http://dx.doi.org/10.1038/s41598-020-68892-5
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author Yardimci, Sevim
Burnham, Daniel R.
Terry, Samantha Y. A.
Yardimci, Hasan
author_facet Yardimci, Sevim
Burnham, Daniel R.
Terry, Samantha Y. A.
Yardimci, Hasan
author_sort Yardimci, Sevim
collection PubMed
description Recent advances in fluorescence super-resolution microscopy are providing important insights into details of cellular structures. To acquire three dimensional (3D) super-resolution images of DNA, we combined binding activated localization microscopy (BALM) using fluorescent double-stranded DNA intercalators and optical astigmatism. We quantitatively establish the advantage of bis- over mono-intercalators before demonstrating the approach by visualizing single DNA molecules stretched between microspheres at various heights. Finally, the approach is applied to the more complex environment of intact and damaged metaphase chromosomes, unravelling their structural features.
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spelling pubmed-73851442020-07-28 Three-dimensional super-resolution fluorescence imaging of DNA Yardimci, Sevim Burnham, Daniel R. Terry, Samantha Y. A. Yardimci, Hasan Sci Rep Article Recent advances in fluorescence super-resolution microscopy are providing important insights into details of cellular structures. To acquire three dimensional (3D) super-resolution images of DNA, we combined binding activated localization microscopy (BALM) using fluorescent double-stranded DNA intercalators and optical astigmatism. We quantitatively establish the advantage of bis- over mono-intercalators before demonstrating the approach by visualizing single DNA molecules stretched between microspheres at various heights. Finally, the approach is applied to the more complex environment of intact and damaged metaphase chromosomes, unravelling their structural features. Nature Publishing Group UK 2020-07-27 /pmc/articles/PMC7385144/ /pubmed/32719468 http://dx.doi.org/10.1038/s41598-020-68892-5 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yardimci, Sevim
Burnham, Daniel R.
Terry, Samantha Y. A.
Yardimci, Hasan
Three-dimensional super-resolution fluorescence imaging of DNA
title Three-dimensional super-resolution fluorescence imaging of DNA
title_full Three-dimensional super-resolution fluorescence imaging of DNA
title_fullStr Three-dimensional super-resolution fluorescence imaging of DNA
title_full_unstemmed Three-dimensional super-resolution fluorescence imaging of DNA
title_short Three-dimensional super-resolution fluorescence imaging of DNA
title_sort three-dimensional super-resolution fluorescence imaging of dna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7385144/
https://www.ncbi.nlm.nih.gov/pubmed/32719468
http://dx.doi.org/10.1038/s41598-020-68892-5
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