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Genomic and Phenotypic Analysis of an ESBL-Producing E. coli ST1159 Clonal Lineage From Wild Birds in Mongolia

BACKGROUND: In addition to the broad dissemination of pathogenic extended-spectrum beta-lactamase (ESBL)-producing Escherichia (E.) coli in human and veterinary medicine and the community, their occurrence in wildlife and the environment is a growing concern. Wild birds in particular often carry cli...

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Autores principales: Schierack, Peter, Heiden, Stefan E., Khan, Muhammad Moman, Nikolaus, Lena, Kolenda, Rafal, Stubbe, Michael, Lkhagvasuren, Davaa, Rödiger, Stefan, Guenther, Sebastian, Schaufler, Katharina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7385280/
https://www.ncbi.nlm.nih.gov/pubmed/32793163
http://dx.doi.org/10.3389/fmicb.2020.01699
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author Schierack, Peter
Heiden, Stefan E.
Khan, Muhammad Moman
Nikolaus, Lena
Kolenda, Rafal
Stubbe, Michael
Lkhagvasuren, Davaa
Rödiger, Stefan
Guenther, Sebastian
Schaufler, Katharina
author_facet Schierack, Peter
Heiden, Stefan E.
Khan, Muhammad Moman
Nikolaus, Lena
Kolenda, Rafal
Stubbe, Michael
Lkhagvasuren, Davaa
Rödiger, Stefan
Guenther, Sebastian
Schaufler, Katharina
author_sort Schierack, Peter
collection PubMed
description BACKGROUND: In addition to the broad dissemination of pathogenic extended-spectrum beta-lactamase (ESBL)-producing Escherichia (E.) coli in human and veterinary medicine and the community, their occurrence in wildlife and the environment is a growing concern. Wild birds in particular often carry clinically relevant ESBL-producing E. coli. OBJECTIVES: We analyzed ESBL-producing and non-ESBL-producing E. coli obtained from wild birds in Mongolia to identify phylogenetic and functional characteristics that would explain the predominance of a particular E. coli clonal lineage in this area. METHODS: We investigated ESBL-producing E. coli using whole-genome sequencing and phylogenetics to describe the population structure, resistance and virulence features and performed phenotypic experiments like biofilm formation and adhesion to epithelial cells. We compared the phenotypic characteristics to non-ESBL-producing E. coli from the same background (Mongolian wild birds) and genomic results to publicly available genomes. RESULTS AND CONCLUSION: We found ESBL-producing E. coli sequence type (ST) 1159 among wild birds in Mongolia. This clonal lineage carried virulence features typical for extra-intestinal pathogenic or enterotoxigenic E. coli. Comparative functional experiments suggested no burden of resistance in the ST1159 isolates, which is despite their carriage of ESBL-plasmids. Wild birds will likely disseminate these antibiotic-resistant pathogens further during migration.
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spelling pubmed-73852802020-08-12 Genomic and Phenotypic Analysis of an ESBL-Producing E. coli ST1159 Clonal Lineage From Wild Birds in Mongolia Schierack, Peter Heiden, Stefan E. Khan, Muhammad Moman Nikolaus, Lena Kolenda, Rafal Stubbe, Michael Lkhagvasuren, Davaa Rödiger, Stefan Guenther, Sebastian Schaufler, Katharina Front Microbiol Microbiology BACKGROUND: In addition to the broad dissemination of pathogenic extended-spectrum beta-lactamase (ESBL)-producing Escherichia (E.) coli in human and veterinary medicine and the community, their occurrence in wildlife and the environment is a growing concern. Wild birds in particular often carry clinically relevant ESBL-producing E. coli. OBJECTIVES: We analyzed ESBL-producing and non-ESBL-producing E. coli obtained from wild birds in Mongolia to identify phylogenetic and functional characteristics that would explain the predominance of a particular E. coli clonal lineage in this area. METHODS: We investigated ESBL-producing E. coli using whole-genome sequencing and phylogenetics to describe the population structure, resistance and virulence features and performed phenotypic experiments like biofilm formation and adhesion to epithelial cells. We compared the phenotypic characteristics to non-ESBL-producing E. coli from the same background (Mongolian wild birds) and genomic results to publicly available genomes. RESULTS AND CONCLUSION: We found ESBL-producing E. coli sequence type (ST) 1159 among wild birds in Mongolia. This clonal lineage carried virulence features typical for extra-intestinal pathogenic or enterotoxigenic E. coli. Comparative functional experiments suggested no burden of resistance in the ST1159 isolates, which is despite their carriage of ESBL-plasmids. Wild birds will likely disseminate these antibiotic-resistant pathogens further during migration. Frontiers Media S.A. 2020-07-21 /pmc/articles/PMC7385280/ /pubmed/32793163 http://dx.doi.org/10.3389/fmicb.2020.01699 Text en Copyright © 2020 Schierack, Heiden, Khan, Nikolaus, Kolenda, Stubbe, Lkhagvasuren, Rödiger, Guenther and Schaufler. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Schierack, Peter
Heiden, Stefan E.
Khan, Muhammad Moman
Nikolaus, Lena
Kolenda, Rafal
Stubbe, Michael
Lkhagvasuren, Davaa
Rödiger, Stefan
Guenther, Sebastian
Schaufler, Katharina
Genomic and Phenotypic Analysis of an ESBL-Producing E. coli ST1159 Clonal Lineage From Wild Birds in Mongolia
title Genomic and Phenotypic Analysis of an ESBL-Producing E. coli ST1159 Clonal Lineage From Wild Birds in Mongolia
title_full Genomic and Phenotypic Analysis of an ESBL-Producing E. coli ST1159 Clonal Lineage From Wild Birds in Mongolia
title_fullStr Genomic and Phenotypic Analysis of an ESBL-Producing E. coli ST1159 Clonal Lineage From Wild Birds in Mongolia
title_full_unstemmed Genomic and Phenotypic Analysis of an ESBL-Producing E. coli ST1159 Clonal Lineage From Wild Birds in Mongolia
title_short Genomic and Phenotypic Analysis of an ESBL-Producing E. coli ST1159 Clonal Lineage From Wild Birds in Mongolia
title_sort genomic and phenotypic analysis of an esbl-producing e. coli st1159 clonal lineage from wild birds in mongolia
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7385280/
https://www.ncbi.nlm.nih.gov/pubmed/32793163
http://dx.doi.org/10.3389/fmicb.2020.01699
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