TEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing

The PIWI protein MIWI2 and its associated PIWI-interacting RNAs (piRNAs) instruct DNA methylation of young active transposable elements (TEs) in the male germline. piRNAs are proposed to recruit MIWI2 to the transcriptionally active TE loci by base pairing to nascent transcripts, however the downstr...

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Detalles Bibliográficos
Autores principales: Schöpp, Theresa, Zoch, Ansgar, Berrens, Rebecca V., Auchynnikava, Tania, Kabayama, Yuka, Vasiliauskaitė, Lina, Rappsilber, Juri, Allshire, Robin C., O’Carroll, Dónal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7385494/
https://www.ncbi.nlm.nih.gov/pubmed/32719317
http://dx.doi.org/10.1038/s41467-020-17372-5
Descripción
Sumario:The PIWI protein MIWI2 and its associated PIWI-interacting RNAs (piRNAs) instruct DNA methylation of young active transposable elements (TEs) in the male germline. piRNAs are proposed to recruit MIWI2 to the transcriptionally active TE loci by base pairing to nascent transcripts, however the downstream mechanisms and effector proteins utilized by MIWI2 in directing de novo TE methylation remain incompletely understood. Here, we show that MIWI2 associates with TEX15 in foetal gonocytes. TEX15 is predominantly a nuclear protein that is not required for piRNA biogenesis but is essential for piRNA-directed TE de novo methylation and silencing. In summary, TEX15 is an essential executor of mammalian piRNA-directed DNA methylation.

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