TEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing

The PIWI protein MIWI2 and its associated PIWI-interacting RNAs (piRNAs) instruct DNA methylation of young active transposable elements (TEs) in the male germline. piRNAs are proposed to recruit MIWI2 to the transcriptionally active TE loci by base pairing to nascent transcripts, however the downstr...

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Autores principales: Schöpp, Theresa, Zoch, Ansgar, Berrens, Rebecca V., Auchynnikava, Tania, Kabayama, Yuka, Vasiliauskaitė, Lina, Rappsilber, Juri, Allshire, Robin C., O’Carroll, Dónal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7385494/
https://www.ncbi.nlm.nih.gov/pubmed/32719317
http://dx.doi.org/10.1038/s41467-020-17372-5
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author Schöpp, Theresa
Zoch, Ansgar
Berrens, Rebecca V.
Auchynnikava, Tania
Kabayama, Yuka
Vasiliauskaitė, Lina
Rappsilber, Juri
Allshire, Robin C.
O’Carroll, Dónal
author_facet Schöpp, Theresa
Zoch, Ansgar
Berrens, Rebecca V.
Auchynnikava, Tania
Kabayama, Yuka
Vasiliauskaitė, Lina
Rappsilber, Juri
Allshire, Robin C.
O’Carroll, Dónal
author_sort Schöpp, Theresa
collection PubMed
description The PIWI protein MIWI2 and its associated PIWI-interacting RNAs (piRNAs) instruct DNA methylation of young active transposable elements (TEs) in the male germline. piRNAs are proposed to recruit MIWI2 to the transcriptionally active TE loci by base pairing to nascent transcripts, however the downstream mechanisms and effector proteins utilized by MIWI2 in directing de novo TE methylation remain incompletely understood. Here, we show that MIWI2 associates with TEX15 in foetal gonocytes. TEX15 is predominantly a nuclear protein that is not required for piRNA biogenesis but is essential for piRNA-directed TE de novo methylation and silencing. In summary, TEX15 is an essential executor of mammalian piRNA-directed DNA methylation.
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spelling pubmed-73854942020-08-12 TEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing Schöpp, Theresa Zoch, Ansgar Berrens, Rebecca V. Auchynnikava, Tania Kabayama, Yuka Vasiliauskaitė, Lina Rappsilber, Juri Allshire, Robin C. O’Carroll, Dónal Nat Commun Article The PIWI protein MIWI2 and its associated PIWI-interacting RNAs (piRNAs) instruct DNA methylation of young active transposable elements (TEs) in the male germline. piRNAs are proposed to recruit MIWI2 to the transcriptionally active TE loci by base pairing to nascent transcripts, however the downstream mechanisms and effector proteins utilized by MIWI2 in directing de novo TE methylation remain incompletely understood. Here, we show that MIWI2 associates with TEX15 in foetal gonocytes. TEX15 is predominantly a nuclear protein that is not required for piRNA biogenesis but is essential for piRNA-directed TE de novo methylation and silencing. In summary, TEX15 is an essential executor of mammalian piRNA-directed DNA methylation. Nature Publishing Group UK 2020-07-27 /pmc/articles/PMC7385494/ /pubmed/32719317 http://dx.doi.org/10.1038/s41467-020-17372-5 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Schöpp, Theresa
Zoch, Ansgar
Berrens, Rebecca V.
Auchynnikava, Tania
Kabayama, Yuka
Vasiliauskaitė, Lina
Rappsilber, Juri
Allshire, Robin C.
O’Carroll, Dónal
TEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing
title TEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing
title_full TEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing
title_fullStr TEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing
title_full_unstemmed TEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing
title_short TEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing
title_sort tex15 is an essential executor of miwi2-directed transposon dna methylation and silencing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7385494/
https://www.ncbi.nlm.nih.gov/pubmed/32719317
http://dx.doi.org/10.1038/s41467-020-17372-5
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