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Efficacy of the fat-dissociation method for nodal harvesting in gastric cancer

BACKGROUND: There is an increased need for accurate staging for gastric cancer treatment. Consequently, it is necessary to carefully examine all dissected lymph nodes for precise staging. Recently, the fat-dissociation method has been developed as a quick and accurate method for harvesting dissected...

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Autores principales: Kinami, Shinichi, Ohnishi, Toshio, Nakamura, Naohiko, Jiang, Zhi Yong, Miyata, Takashi, Fujita, Hideto, Takamura, Hiroyuki, Ueda, Nobuhiko, Kosaka, Takeo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Baishideng Publishing Group Inc 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7385510/
https://www.ncbi.nlm.nih.gov/pubmed/32774766
http://dx.doi.org/10.4240/wjgs.v12.i6.277
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author Kinami, Shinichi
Ohnishi, Toshio
Nakamura, Naohiko
Jiang, Zhi Yong
Miyata, Takashi
Fujita, Hideto
Takamura, Hiroyuki
Ueda, Nobuhiko
Kosaka, Takeo
author_facet Kinami, Shinichi
Ohnishi, Toshio
Nakamura, Naohiko
Jiang, Zhi Yong
Miyata, Takashi
Fujita, Hideto
Takamura, Hiroyuki
Ueda, Nobuhiko
Kosaka, Takeo
author_sort Kinami, Shinichi
collection PubMed
description BACKGROUND: There is an increased need for accurate staging for gastric cancer treatment. Consequently, it is necessary to carefully examine all dissected lymph nodes for precise staging. Recently, the fat-dissociation method has been developed as a quick and accurate method for harvesting dissected lymph nodes of colorectal cancer cases. AIM: To investigate the usefulness of the fat-dissociation method for harvesting dissected lymph nodes of gastric cancer cases. METHODS: Fifty-six resected specimens from gastric cancer patients who underwent standard curative gastrectomy and lymph node dissection at our hospital were used. Group 2 lymph nodes were separated from each specimen, and the remaining adipose tissue containing the group 1 lymph nodes was used. Some resected specimens were subjected to the fat-dissociation method. One vial of Imofully(®) was dissolved in 50 mL of saline and injected into the tissue. The tissue was incubated for 1 h and the dissolved fat was removed. Subsequently, the nodes were identified, picked up with scissors, and mapped. The number of nodes in each lymphatic compartment and duration of lymph node harvest and mapping were compared. RESULTS: The fat-dissociation method was used for 24 samples, while the conventional dissection method was used for 32 samples. The total number of harvested lymph nodes was 45.9 in the fat dissociation group and 44.3 in the control group, and there was no significant difference between the two groups. There were also no significant differences in the number of lymph nodes between the two groups based on a comparison of the lymphatic compartments. However, the total median duration of the fat-dissociation method was 38.2 min, reflecting a reduced duration of approximately 60 min compared to the control group. CONCLUSION: Based on our results, the fat-dissociation method is effective in shortening the duration of lymph node harvest in gastric cancer surgery.
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spelling pubmed-73855102020-08-07 Efficacy of the fat-dissociation method for nodal harvesting in gastric cancer Kinami, Shinichi Ohnishi, Toshio Nakamura, Naohiko Jiang, Zhi Yong Miyata, Takashi Fujita, Hideto Takamura, Hiroyuki Ueda, Nobuhiko Kosaka, Takeo World J Gastrointest Surg Retrospective Study BACKGROUND: There is an increased need for accurate staging for gastric cancer treatment. Consequently, it is necessary to carefully examine all dissected lymph nodes for precise staging. Recently, the fat-dissociation method has been developed as a quick and accurate method for harvesting dissected lymph nodes of colorectal cancer cases. AIM: To investigate the usefulness of the fat-dissociation method for harvesting dissected lymph nodes of gastric cancer cases. METHODS: Fifty-six resected specimens from gastric cancer patients who underwent standard curative gastrectomy and lymph node dissection at our hospital were used. Group 2 lymph nodes were separated from each specimen, and the remaining adipose tissue containing the group 1 lymph nodes was used. Some resected specimens were subjected to the fat-dissociation method. One vial of Imofully(®) was dissolved in 50 mL of saline and injected into the tissue. The tissue was incubated for 1 h and the dissolved fat was removed. Subsequently, the nodes were identified, picked up with scissors, and mapped. The number of nodes in each lymphatic compartment and duration of lymph node harvest and mapping were compared. RESULTS: The fat-dissociation method was used for 24 samples, while the conventional dissection method was used for 32 samples. The total number of harvested lymph nodes was 45.9 in the fat dissociation group and 44.3 in the control group, and there was no significant difference between the two groups. There were also no significant differences in the number of lymph nodes between the two groups based on a comparison of the lymphatic compartments. However, the total median duration of the fat-dissociation method was 38.2 min, reflecting a reduced duration of approximately 60 min compared to the control group. CONCLUSION: Based on our results, the fat-dissociation method is effective in shortening the duration of lymph node harvest in gastric cancer surgery. Baishideng Publishing Group Inc 2020-06-27 2020-06-27 /pmc/articles/PMC7385510/ /pubmed/32774766 http://dx.doi.org/10.4240/wjgs.v12.i6.277 Text en ©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.
spellingShingle Retrospective Study
Kinami, Shinichi
Ohnishi, Toshio
Nakamura, Naohiko
Jiang, Zhi Yong
Miyata, Takashi
Fujita, Hideto
Takamura, Hiroyuki
Ueda, Nobuhiko
Kosaka, Takeo
Efficacy of the fat-dissociation method for nodal harvesting in gastric cancer
title Efficacy of the fat-dissociation method for nodal harvesting in gastric cancer
title_full Efficacy of the fat-dissociation method for nodal harvesting in gastric cancer
title_fullStr Efficacy of the fat-dissociation method for nodal harvesting in gastric cancer
title_full_unstemmed Efficacy of the fat-dissociation method for nodal harvesting in gastric cancer
title_short Efficacy of the fat-dissociation method for nodal harvesting in gastric cancer
title_sort efficacy of the fat-dissociation method for nodal harvesting in gastric cancer
topic Retrospective Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7385510/
https://www.ncbi.nlm.nih.gov/pubmed/32774766
http://dx.doi.org/10.4240/wjgs.v12.i6.277
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