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Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses
Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-β (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sens...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
The Author(s). Published by Elsevier B.V.
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7386308/ https://www.ncbi.nlm.nih.gov/pubmed/32735900 http://dx.doi.org/10.1016/j.antiviral.2020.104874 |
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| author | Prasanth, K. Reddisiva Hirano, Minato Fagg, W. Samuel McAnarney, Eileen T. Shan, Chao Xie, Xuping Hage, Adam Pietzsch, Colette A. Bukreyev, Alexander Rajsbaum, Ricardo Shi, Pei-Yong Bedford, Mark T. Bradrick, Shelton S. Menachery, Vineet Garcia-Blanco, Mariano A. |
| author_facet | Prasanth, K. Reddisiva Hirano, Minato Fagg, W. Samuel McAnarney, Eileen T. Shan, Chao Xie, Xuping Hage, Adam Pietzsch, Colette A. Bukreyev, Alexander Rajsbaum, Ricardo Shi, Pei-Yong Bedford, Mark T. Bradrick, Shelton S. Menachery, Vineet Garcia-Blanco, Mariano A. |
| author_sort | Prasanth, K. Reddisiva |
| collection | PubMed |
| description | Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-β (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target. |
| format | Online Article Text |
| id | pubmed-7386308 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2020 |
| publisher | The Author(s). Published by Elsevier B.V. |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-73863082020-07-29 Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses Prasanth, K. Reddisiva Hirano, Minato Fagg, W. Samuel McAnarney, Eileen T. Shan, Chao Xie, Xuping Hage, Adam Pietzsch, Colette A. Bukreyev, Alexander Rajsbaum, Ricardo Shi, Pei-Yong Bedford, Mark T. Bradrick, Shelton S. Menachery, Vineet Garcia-Blanco, Mariano A. Antiviral Res Short Communication Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-β (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target. The Author(s). Published by Elsevier B.V. 2020-10 2020-07-28 /pmc/articles/PMC7386308/ /pubmed/32735900 http://dx.doi.org/10.1016/j.antiviral.2020.104874 Text en © 2020 The Author(s) Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
| spellingShingle | Short Communication Prasanth, K. Reddisiva Hirano, Minato Fagg, W. Samuel McAnarney, Eileen T. Shan, Chao Xie, Xuping Hage, Adam Pietzsch, Colette A. Bukreyev, Alexander Rajsbaum, Ricardo Shi, Pei-Yong Bedford, Mark T. Bradrick, Shelton S. Menachery, Vineet Garcia-Blanco, Mariano A. Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses |
| title | Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses |
| title_full | Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses |
| title_fullStr | Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses |
| title_full_unstemmed | Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses |
| title_short | Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses |
| title_sort | topoisomerase iii-β is required for efficient replication of positive-sense rna viruses |
| topic | Short Communication |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7386308/ https://www.ncbi.nlm.nih.gov/pubmed/32735900 http://dx.doi.org/10.1016/j.antiviral.2020.104874 |
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