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Dysregulated transcriptional responses to SARS-CoV-2 in the periphery support novel diagnostic approaches
In order to elucidate novel aspects of the host response to SARS-CoV-2 we performed RNA sequencing on peripheral blood samples across 77 timepoints from 46 subjects with COVID-19 and compared them to subjects with seasonal coronavirus, influenza, bacterial pneumonia, and healthy controls. Early SARS...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7386527/ https://www.ncbi.nlm.nih.gov/pubmed/32743603 http://dx.doi.org/10.1101/2020.07.20.20155507 |
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author | McClain, Micah T. Constantine, Florica J. Henao, Ricardo Liu, Yiling Tsalik, Ephraim L. Burke, Thomas W. Steinbrink, Julie M. Petzold, Elizabeth Nicholson, Bradly P. Rolfe, Robert Kraft, Bryan D. Kelly, Matthew S. Sempowski, Gregory D. Denny, Thomas N. Ginsburg, Geoffrey S. Woods, Christopher W. |
author_facet | McClain, Micah T. Constantine, Florica J. Henao, Ricardo Liu, Yiling Tsalik, Ephraim L. Burke, Thomas W. Steinbrink, Julie M. Petzold, Elizabeth Nicholson, Bradly P. Rolfe, Robert Kraft, Bryan D. Kelly, Matthew S. Sempowski, Gregory D. Denny, Thomas N. Ginsburg, Geoffrey S. Woods, Christopher W. |
author_sort | McClain, Micah T. |
collection | PubMed |
description | In order to elucidate novel aspects of the host response to SARS-CoV-2 we performed RNA sequencing on peripheral blood samples across 77 timepoints from 46 subjects with COVID-19 and compared them to subjects with seasonal coronavirus, influenza, bacterial pneumonia, and healthy controls. Early SARS-CoV-2 infection triggers a conserved transcriptomic response in peripheral blood that is heavily interferon-driven but also marked by indicators of early B-cell activation and antibody production. Interferon responses during SARS-CoV-2 infection demonstrate unique patterns of dysregulated expression compared to other infectious and healthy states. Heterogeneous activation of coagulation and fibrinolytic pathways are present in early COVID-19, as are IL1 and JAK/STAT signaling pathways, that persist into late disease. Classifiers based on differentially expressed genes accurately distinguished SARS-CoV-2 infection from other acute illnesses (auROC 0.95). The transcriptome in peripheral blood reveals unique aspects of the immune response in COVID-19 and provides for novel biomarker-based approaches to diagnosis. |
format | Online Article Text |
id | pubmed-7386527 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Cold Spring Harbor Laboratory |
record_format | MEDLINE/PubMed |
spelling | pubmed-73865272020-07-31 Dysregulated transcriptional responses to SARS-CoV-2 in the periphery support novel diagnostic approaches McClain, Micah T. Constantine, Florica J. Henao, Ricardo Liu, Yiling Tsalik, Ephraim L. Burke, Thomas W. Steinbrink, Julie M. Petzold, Elizabeth Nicholson, Bradly P. Rolfe, Robert Kraft, Bryan D. Kelly, Matthew S. Sempowski, Gregory D. Denny, Thomas N. Ginsburg, Geoffrey S. Woods, Christopher W. medRxiv Article In order to elucidate novel aspects of the host response to SARS-CoV-2 we performed RNA sequencing on peripheral blood samples across 77 timepoints from 46 subjects with COVID-19 and compared them to subjects with seasonal coronavirus, influenza, bacterial pneumonia, and healthy controls. Early SARS-CoV-2 infection triggers a conserved transcriptomic response in peripheral blood that is heavily interferon-driven but also marked by indicators of early B-cell activation and antibody production. Interferon responses during SARS-CoV-2 infection demonstrate unique patterns of dysregulated expression compared to other infectious and healthy states. Heterogeneous activation of coagulation and fibrinolytic pathways are present in early COVID-19, as are IL1 and JAK/STAT signaling pathways, that persist into late disease. Classifiers based on differentially expressed genes accurately distinguished SARS-CoV-2 infection from other acute illnesses (auROC 0.95). The transcriptome in peripheral blood reveals unique aspects of the immune response in COVID-19 and provides for novel biomarker-based approaches to diagnosis. Cold Spring Harbor Laboratory 2020-07-26 /pmc/articles/PMC7386527/ /pubmed/32743603 http://dx.doi.org/10.1101/2020.07.20.20155507 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/It is made available under a CC-BY-NC-ND 4.0 International license (http://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Article McClain, Micah T. Constantine, Florica J. Henao, Ricardo Liu, Yiling Tsalik, Ephraim L. Burke, Thomas W. Steinbrink, Julie M. Petzold, Elizabeth Nicholson, Bradly P. Rolfe, Robert Kraft, Bryan D. Kelly, Matthew S. Sempowski, Gregory D. Denny, Thomas N. Ginsburg, Geoffrey S. Woods, Christopher W. Dysregulated transcriptional responses to SARS-CoV-2 in the periphery support novel diagnostic approaches |
title | Dysregulated transcriptional responses to SARS-CoV-2 in the periphery support novel diagnostic approaches |
title_full | Dysregulated transcriptional responses to SARS-CoV-2 in the periphery support novel diagnostic approaches |
title_fullStr | Dysregulated transcriptional responses to SARS-CoV-2 in the periphery support novel diagnostic approaches |
title_full_unstemmed | Dysregulated transcriptional responses to SARS-CoV-2 in the periphery support novel diagnostic approaches |
title_short | Dysregulated transcriptional responses to SARS-CoV-2 in the periphery support novel diagnostic approaches |
title_sort | dysregulated transcriptional responses to sars-cov-2 in the periphery support novel diagnostic approaches |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7386527/ https://www.ncbi.nlm.nih.gov/pubmed/32743603 http://dx.doi.org/10.1101/2020.07.20.20155507 |
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