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Penicillin-binding proteins regulate multiple steps in the polarized cell division process of Chlamydia
Chlamydia trachomatis serovar L2 and Chlamydia muridarum, which do not express FtsZ, undergo polarized cell division. During division, peptidoglycan assembles at the pole of dividing Chlamydia trachomatis cells where daughter cell formation occurs, and peptidoglycan regulates at least two distinct s...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7387471/ https://www.ncbi.nlm.nih.gov/pubmed/32724139 http://dx.doi.org/10.1038/s41598-020-69397-x |
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author | Cox, John V. Abdelrahman, Yasser Mohamed Ouellette, Scot P. |
author_facet | Cox, John V. Abdelrahman, Yasser Mohamed Ouellette, Scot P. |
author_sort | Cox, John V. |
collection | PubMed |
description | Chlamydia trachomatis serovar L2 and Chlamydia muridarum, which do not express FtsZ, undergo polarized cell division. During division, peptidoglycan assembles at the pole of dividing Chlamydia trachomatis cells where daughter cell formation occurs, and peptidoglycan regulates at least two distinct steps in the polarized division of Chlamydia trachomatis and Chlamydia muridarum. Cells treated with inhibitors that prevent peptidoglycan synthesis or peptidoglycan crosslinking by penicillin-binding protein 2 (PBP2) are unable to initiate polarized division, while cells treated with inhibitors that prevent peptidoglycan crosslinking by penicillin-binding protein 3 (PBP3/FtsI) initiate polarized division, but the process arrests at an early stage of daughter cell growth. Consistent with their distinct roles in polarized division, peptidoglycan organization is different in cells treated with PBP2 and PBP3-specific inhibitors. Our analyses indicate that the sequential action of PBP2 and PBP3 drives changes in peptidoglycan organization that are essential for the polarized division of these obligate intracellular bacteria. Furthermore, the roles we have characterized for PBP2 and PBP3 in regulating specific steps in chlamydial cell division have not been described in other bacteria. |
format | Online Article Text |
id | pubmed-7387471 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-73874712020-07-29 Penicillin-binding proteins regulate multiple steps in the polarized cell division process of Chlamydia Cox, John V. Abdelrahman, Yasser Mohamed Ouellette, Scot P. Sci Rep Article Chlamydia trachomatis serovar L2 and Chlamydia muridarum, which do not express FtsZ, undergo polarized cell division. During division, peptidoglycan assembles at the pole of dividing Chlamydia trachomatis cells where daughter cell formation occurs, and peptidoglycan regulates at least two distinct steps in the polarized division of Chlamydia trachomatis and Chlamydia muridarum. Cells treated with inhibitors that prevent peptidoglycan synthesis or peptidoglycan crosslinking by penicillin-binding protein 2 (PBP2) are unable to initiate polarized division, while cells treated with inhibitors that prevent peptidoglycan crosslinking by penicillin-binding protein 3 (PBP3/FtsI) initiate polarized division, but the process arrests at an early stage of daughter cell growth. Consistent with their distinct roles in polarized division, peptidoglycan organization is different in cells treated with PBP2 and PBP3-specific inhibitors. Our analyses indicate that the sequential action of PBP2 and PBP3 drives changes in peptidoglycan organization that are essential for the polarized division of these obligate intracellular bacteria. Furthermore, the roles we have characterized for PBP2 and PBP3 in regulating specific steps in chlamydial cell division have not been described in other bacteria. Nature Publishing Group UK 2020-07-28 /pmc/articles/PMC7387471/ /pubmed/32724139 http://dx.doi.org/10.1038/s41598-020-69397-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Cox, John V. Abdelrahman, Yasser Mohamed Ouellette, Scot P. Penicillin-binding proteins regulate multiple steps in the polarized cell division process of Chlamydia |
title | Penicillin-binding proteins regulate multiple steps in the polarized cell division process of Chlamydia |
title_full | Penicillin-binding proteins regulate multiple steps in the polarized cell division process of Chlamydia |
title_fullStr | Penicillin-binding proteins regulate multiple steps in the polarized cell division process of Chlamydia |
title_full_unstemmed | Penicillin-binding proteins regulate multiple steps in the polarized cell division process of Chlamydia |
title_short | Penicillin-binding proteins regulate multiple steps in the polarized cell division process of Chlamydia |
title_sort | penicillin-binding proteins regulate multiple steps in the polarized cell division process of chlamydia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7387471/ https://www.ncbi.nlm.nih.gov/pubmed/32724139 http://dx.doi.org/10.1038/s41598-020-69397-x |
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