Cargando…

Evaluating the quantity, quality and size distribution of cell-free DNA by multiplex droplet digital PCR

Cell-free DNA (cfDNA) has become a comprehensive biomarker in the fields of non-invasive cancer detection and monitoring, organ transplantation, prenatal genetic testing and pathogen detection. While cfDNA samples can be obtained using a broad variety of approaches, there is an urgent need to standa...

Descripción completa

Detalles Bibliográficos
Autores principales:	Alcaide, Miguel, Cheung, Matthew, Hillman, Jack, Rassekh, S. Rod, Deyell, Rebecca J., Batist, Gerald, Karsan, Aly, Wyatt, Alexander W., Johnson, Nathalie, Scott, David W., Morin, Ryan D.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7387491/ https://www.ncbi.nlm.nih.gov/pubmed/32724107 http://dx.doi.org/10.1038/s41598-020-69432-x

Ejemplares similares

Targeted error-suppressed quantification of circulating tumor DNA using semi-degenerate barcoded adapters and biotinylated baits
por: Alcaide, Miguel, et al.
Publicado: (2017)

HIV reservoir quantification by five-target multiplex droplet digital PCR
por: Levy, Claire N., et al.
Publicado: (2021)

Multiplex detection of “Candidatus Liberibacter asiaticus” and Spiroplasma citri by qPCR and droplet digital PCR
por: Maheshwari, Yogita, et al.
Publicado: (2021)

Detection and Quantification of Adulterated Beef and Mutton Products by Multiplex Droplet Digital PCR
por: He, Chuan, et al.
Publicado: (2022)

A highly multiplexed droplet digital PCR assay to measure the intact HIV-1 proviral reservoir
por: Levy, Claire N., et al.
Publicado: (2021)

Multiplex Detection of Rare Mutations by Picoliter Droplet Based Digital PCR: Sensitivity and Specificity Considerations
por: Zonta, Eleonora, et al.
Publicado: (2016)

Sensitive detection and quantification of SARS-CoV-2 by multiplex droplet digital RT-PCR
por: de Kock, Remco, et al.
Publicado: (2020)

Development of a Multiplex Droplet Digital PCR Assay for the Detection of Babesia, Bartonella, and Borrelia Species
por: Maggi, Ricardo, et al.
Publicado: (2021)

Increasing the Efficiency of Canola and Soybean GMO Detection and Quantification Using Multiplex Droplet Digital PCR
por: Demeke, Tigst, et al.
Publicado: (2022)

Bisulfite-Converted DNA Quantity Evaluation: A Multiplex Quantitative Real-Time PCR System for Evaluation of Bisulfite Conversion
por: Hong, Sae Rom, et al.
Publicado: (2021)

Clinical Application of a Multiplex Droplet Digital PCR in the Rapid Diagnosis of Children with Suspected Bloodstream Infections
por: Liu, Wenxin, et al.
Publicado: (2023)

Multiplexed Illumina sequencing libraries from picogram quantities of DNA
por: Bowman, Sarah K, et al.
Publicado: (2013)

Multiplexed Single Intact Cell Droplet Digital PCR (MuSIC ddPCR) Method for Specific Detection of Enterohemorrhagic E. coli (EHEC) in Food Enrichment Cultures
por: McMahon, Tanis C., et al.
Publicado: (2017)

Detection of clinically relevant copy number alterations in oral cancer progression using multiplexed droplet digital PCR
por: Hughesman, Curtis B., et al.
Publicado: (2017)

Multiplexed Droplet Digital PCR Assays for the Simultaneous Screening of Major Genetic Alterations in Tumors of the Central Nervous System
por: Appay, Romain, et al.
Publicado: (2020)

A multiplexed, paired-pooled droplet digital PCR assay for detection of SARS-CoV-2 in saliva
por: Wagner, Kaitlyn, et al.
Publicado: (2023)

Detection and quantification of integrated vector copy number by multiplex droplet digital PCR in dual-transduced CAR T cells
por: Wang, Wei, et al.
Publicado: (2023)

Utilization of Digital PCR in Quantity Verification of Plasmid Standards Used in Quantitative PCR
por: Beinhauerova, Martina, et al.
Publicado: (2020)

Interferometric laser imaging for respiratory droplets sizing
por: Grandoni, Livia, et al.
Publicado: (2023)

Multiplex quantification of four DNA targets in one reaction with Bio-Rad droplet digital PCR system for GMO detection
por: Dobnik, David, et al.
Publicado: (2016)

Optimisation of robust singleplex and multiplex droplet digital PCR assays for high confidence mutation detection in circulating tumour DNA
por: Rowlands, Vicky, et al.
Publicado: (2019)

Development and inter-laboratory assessment of droplet digital PCR assays for multiplex quantification of 15 genetically modified soybean lines
por: Košir, Alexandra Bogožalec, et al.
Publicado: (2017)

Ultrafast multiplexed detection of SARS-CoV-2 RNA using a rapid droplet digital PCR system
por: Yin, Hao, et al.
Publicado: (2021)

Absolute quantification of viable Vibrio cholerae in seawater samples using multiplex droplet digital PCR combined with propidium monoazide
por: Yang, Jinsong, et al.
Publicado: (2023)

A new multiplexed magnetic capture—Droplet digital PCR tool for monitoring wildlife population health and pathogen surveillance
por: Tschritter, Christina M., et al.
Publicado: (2023)

Multiplex immunoassay analysis of biomarkers in clinically accessible quantities of human aqueous humor
por: Sharma, Rajesh K., et al.
Publicado: (2009)

High-throughput multiplexed fluorescence-activated droplet sorting
por: Caen, Ouriel, et al.
Publicado: (2018)

Mutation screening in 86 known X-linked mental retardation genes by droplet-based multiplex PCR and massive parallel sequencing
por: Hu, Hao, et al.
Publicado: (2010)

Efficient Genotyping of KRAS Mutant Non-Small Cell Lung Cancer Using a Multiplexed Droplet Digital PCR Approach
por: Pender, Alexandra, et al.
Publicado: (2015)

A Robust Protocol for Using Multiplexed Droplet Digital PCR to Quantify Somatic Copy Number Alterations in Clinical Tissue Specimens
por: Hughesman, Curtis B., et al.
Publicado: (2016)

Biomarkers of Castrate Resistance in Prostate Cancer: Androgen Receptor Amplification and T877A Mutation Detection by Multiplex Droplet Digital PCR
por: Young, Francis P., et al.
Publicado: (2022)

Multiplex Droplet Digital PCR Assay for Detection of MET and HER2 Genes Amplification in Non-Small Cell Lung Cancer
por: Oscorbin, Igor P., et al.
Publicado: (2022)

Non-invasive prenatal testing for fetal Ss, Kidd, and CTL2 blood group prediction by multiplex digital droplet PCR
por: Wang, Yufeng, et al.
Publicado: (2023)

Transgene Detection by Digital Droplet PCR
por: Moser, Dirk A., et al.
Publicado: (2014)

Fundamentals of multiplexing with digital PCR
por: Whale, Alexandra S., et al.
Publicado: (2016)

Influence of Current Feeding Position of Duplex Current Feeding MIG Welding on Droplet Heat Quantity
por: Aoki, Atsuhito, et al.
Publicado: (2019)

Real-time multiplex PCR for human echinococcosis and differential diagnosis
por: Knapp, Jenny, et al.
Publicado: (2023)

Erratum to: Mutation screening in 86 known X-linked mental retardation genes by droplet-based multiplex PCR and massive parallel sequencing
por: Hu, Hao, et al.
Publicado: (2010)

Author Correction: Development and inter-laboratory assessment of droplet digital PCR assays for multiplex quantification of 15 genetically modified soybean lines
por: Košir, Alexandra Bogožalec, et al.
Publicado: (2019)

Clinical validation of a multiplex droplet digital PCR for diagnosing suspected bloodstream infections in ICU practice: a promising diagnostic tool
por: Wu, Jing, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_hg3gm10c29p1907rt515sd37q6