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C22 podovirus infectivity is associated with intermediate stiffness

Bacteriophages have potential for use as biological control agents (biocontrols) of pathogenic bacteria, but their low stability is limiting for their utilization as biocontrols. Understanding of the conditions conducive to storage of phages in which infectivity is maintained over long periods will...

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Autores principales: Sae-Ueng, Udom, Bhunchoth, Anjana, Phironrit, Namthip, Treetong, Alongkot, Sapcharoenkun, Chaweewan, Chatchawankanphanich, Orawan, Leartsakulpanich, Ubolsree, Chitnumsub, Penchit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7387534/
https://www.ncbi.nlm.nih.gov/pubmed/32724109
http://dx.doi.org/10.1038/s41598-020-69409-w
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author Sae-Ueng, Udom
Bhunchoth, Anjana
Phironrit, Namthip
Treetong, Alongkot
Sapcharoenkun, Chaweewan
Chatchawankanphanich, Orawan
Leartsakulpanich, Ubolsree
Chitnumsub, Penchit
author_facet Sae-Ueng, Udom
Bhunchoth, Anjana
Phironrit, Namthip
Treetong, Alongkot
Sapcharoenkun, Chaweewan
Chatchawankanphanich, Orawan
Leartsakulpanich, Ubolsree
Chitnumsub, Penchit
author_sort Sae-Ueng, Udom
collection PubMed
description Bacteriophages have potential for use as biological control agents (biocontrols) of pathogenic bacteria, but their low stability is limiting for their utilization as biocontrols. Understanding of the conditions conducive to storage of phages in which infectivity is maintained over long periods will be useful for their application as biocontrols. We employed a nanomechanical approach to study how external environmental factors affect surface properties and infectivity of the podovirus C22 phage, a candidate for biocontrol of Ralstonia solanacearum, the agent of bacterial wilt in crops. We performed atomic force microscopy (AFM)-based nano-indentation on the C22 phage in buffers with varying pH and ionic strength. The infectivity data from plaque assay in the same conditions revealed that an intermediate range of stiffness was associated with phage titer that remained consistently high, even after prolonged storage up to 182 days. The data are consistent with the model that C22 phage must adopt a metastable state for maximal infectivity, and external factors that alter the stiffness of the phage capsid lead to perturbation of this infective state.
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spelling pubmed-73875342020-07-29 C22 podovirus infectivity is associated with intermediate stiffness Sae-Ueng, Udom Bhunchoth, Anjana Phironrit, Namthip Treetong, Alongkot Sapcharoenkun, Chaweewan Chatchawankanphanich, Orawan Leartsakulpanich, Ubolsree Chitnumsub, Penchit Sci Rep Article Bacteriophages have potential for use as biological control agents (biocontrols) of pathogenic bacteria, but their low stability is limiting for their utilization as biocontrols. Understanding of the conditions conducive to storage of phages in which infectivity is maintained over long periods will be useful for their application as biocontrols. We employed a nanomechanical approach to study how external environmental factors affect surface properties and infectivity of the podovirus C22 phage, a candidate for biocontrol of Ralstonia solanacearum, the agent of bacterial wilt in crops. We performed atomic force microscopy (AFM)-based nano-indentation on the C22 phage in buffers with varying pH and ionic strength. The infectivity data from plaque assay in the same conditions revealed that an intermediate range of stiffness was associated with phage titer that remained consistently high, even after prolonged storage up to 182 days. The data are consistent with the model that C22 phage must adopt a metastable state for maximal infectivity, and external factors that alter the stiffness of the phage capsid lead to perturbation of this infective state. Nature Publishing Group UK 2020-07-28 /pmc/articles/PMC7387534/ /pubmed/32724109 http://dx.doi.org/10.1038/s41598-020-69409-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sae-Ueng, Udom
Bhunchoth, Anjana
Phironrit, Namthip
Treetong, Alongkot
Sapcharoenkun, Chaweewan
Chatchawankanphanich, Orawan
Leartsakulpanich, Ubolsree
Chitnumsub, Penchit
C22 podovirus infectivity is associated with intermediate stiffness
title C22 podovirus infectivity is associated with intermediate stiffness
title_full C22 podovirus infectivity is associated with intermediate stiffness
title_fullStr C22 podovirus infectivity is associated with intermediate stiffness
title_full_unstemmed C22 podovirus infectivity is associated with intermediate stiffness
title_short C22 podovirus infectivity is associated with intermediate stiffness
title_sort c22 podovirus infectivity is associated with intermediate stiffness
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7387534/
https://www.ncbi.nlm.nih.gov/pubmed/32724109
http://dx.doi.org/10.1038/s41598-020-69409-w
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