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Vitamin D Supplementation in Laboratory-Bred Mice: An In Vivo Assay on Gut Microbiome and Body Weight

Saudi Arabia is in a tropical geographical region with a population that has access to adequate diet. There is, however, a high level of vitamin D deficiency in the Kingdom, comorbid with other disease. There is the postulation of a correlation between a healthy gut microbiota and balanced levels of...

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Autores principales: Badger-Emeka, Lorina Ineta, AlJaziri, Zainab Yaseen, Almulhim, Cereen Fahad, Aldrees, Asma Saleh, AlShakhs, Zainab Hamzah, AlAithan, Reem Ibrahim, Alothman, Fatimah Abdullah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7388085/
https://www.ncbi.nlm.nih.gov/pubmed/32782431
http://dx.doi.org/10.1177/1178636120945294
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author Badger-Emeka, Lorina Ineta
AlJaziri, Zainab Yaseen
Almulhim, Cereen Fahad
Aldrees, Asma Saleh
AlShakhs, Zainab Hamzah
AlAithan, Reem Ibrahim
Alothman, Fatimah Abdullah
author_facet Badger-Emeka, Lorina Ineta
AlJaziri, Zainab Yaseen
Almulhim, Cereen Fahad
Aldrees, Asma Saleh
AlShakhs, Zainab Hamzah
AlAithan, Reem Ibrahim
Alothman, Fatimah Abdullah
author_sort Badger-Emeka, Lorina Ineta
collection PubMed
description Saudi Arabia is in a tropical geographical region with a population that has access to adequate diet. There is, however, a high level of vitamin D deficiency in the Kingdom, comorbid with other disease. There is the postulation of a correlation between a healthy gut microbiota and balanced levels of serum vitamin D. This investigation looks into the effect of vitamin D supplementation on the gut flora of laboratory-bred mice as well as any possible association on body weight. BALB/C mice weighing between 34 and 35.8 g were divided into 4 groups and placed on daily doses of vitamin D of 3.75 µg (low dose), 7.5 µg (normal dose), and 15 µg (high dose). The fourth group was the control group that did not receive any supplementation with vitamin D. Body weights were monitored on weekly basis, while faecal samples from the rectum were obtained for microbial culturing and the monitoring of bacterial colony count using the Vitek 2 Compact automated system (BioMerieux, Marcy-l’Etoile, France) according to manufacturer’s guidelines. The data presented as mean ± SD, while significant differences were determined with 2-way analysis of variance in comparing differences within and between treatment groups. The different doses of vitamin D showed varying effects on the body weight and gut microbial colonies of the mice. There was a highly significant difference between the control, 15 µg (high), and 7.5 µg (normal) dose groups. This is suggestive that supplementation with vitamin D could a role in the gut microbial flora in the gut which could reflect in changes in body weight.
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spelling pubmed-73880852020-08-10 Vitamin D Supplementation in Laboratory-Bred Mice: An In Vivo Assay on Gut Microbiome and Body Weight Badger-Emeka, Lorina Ineta AlJaziri, Zainab Yaseen Almulhim, Cereen Fahad Aldrees, Asma Saleh AlShakhs, Zainab Hamzah AlAithan, Reem Ibrahim Alothman, Fatimah Abdullah Microbiol Insights Original Research Saudi Arabia is in a tropical geographical region with a population that has access to adequate diet. There is, however, a high level of vitamin D deficiency in the Kingdom, comorbid with other disease. There is the postulation of a correlation between a healthy gut microbiota and balanced levels of serum vitamin D. This investigation looks into the effect of vitamin D supplementation on the gut flora of laboratory-bred mice as well as any possible association on body weight. BALB/C mice weighing between 34 and 35.8 g were divided into 4 groups and placed on daily doses of vitamin D of 3.75 µg (low dose), 7.5 µg (normal dose), and 15 µg (high dose). The fourth group was the control group that did not receive any supplementation with vitamin D. Body weights were monitored on weekly basis, while faecal samples from the rectum were obtained for microbial culturing and the monitoring of bacterial colony count using the Vitek 2 Compact automated system (BioMerieux, Marcy-l’Etoile, France) according to manufacturer’s guidelines. The data presented as mean ± SD, while significant differences were determined with 2-way analysis of variance in comparing differences within and between treatment groups. The different doses of vitamin D showed varying effects on the body weight and gut microbial colonies of the mice. There was a highly significant difference between the control, 15 µg (high), and 7.5 µg (normal) dose groups. This is suggestive that supplementation with vitamin D could a role in the gut microbial flora in the gut which could reflect in changes in body weight. SAGE Publications 2020-07-28 /pmc/articles/PMC7388085/ /pubmed/32782431 http://dx.doi.org/10.1177/1178636120945294 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Research
Badger-Emeka, Lorina Ineta
AlJaziri, Zainab Yaseen
Almulhim, Cereen Fahad
Aldrees, Asma Saleh
AlShakhs, Zainab Hamzah
AlAithan, Reem Ibrahim
Alothman, Fatimah Abdullah
Vitamin D Supplementation in Laboratory-Bred Mice: An In Vivo Assay on Gut Microbiome and Body Weight
title Vitamin D Supplementation in Laboratory-Bred Mice: An In Vivo Assay on Gut Microbiome and Body Weight
title_full Vitamin D Supplementation in Laboratory-Bred Mice: An In Vivo Assay on Gut Microbiome and Body Weight
title_fullStr Vitamin D Supplementation in Laboratory-Bred Mice: An In Vivo Assay on Gut Microbiome and Body Weight
title_full_unstemmed Vitamin D Supplementation in Laboratory-Bred Mice: An In Vivo Assay on Gut Microbiome and Body Weight
title_short Vitamin D Supplementation in Laboratory-Bred Mice: An In Vivo Assay on Gut Microbiome and Body Weight
title_sort vitamin d supplementation in laboratory-bred mice: an in vivo assay on gut microbiome and body weight
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7388085/
https://www.ncbi.nlm.nih.gov/pubmed/32782431
http://dx.doi.org/10.1177/1178636120945294
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