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lncRNA TTN-AS1 upregulates RUNX1 to enhance glioma progression via sponging miR-27b-3p

Long non-coding RNAs (lncRNAs) contribute to the tumorigeneses of numerous types of cancer, including glioma. The present study was designed to unveil a novel lncRNA functioning in glioma and explore the underlying mechanisms. lncRNA titin-antisense RNA1 (TTN-AS1), miR-27b-3p and Runt-related transc...

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Autores principales: Chang, Keliang, Wang, Genwei, Lou, Jinfeng, Hao, Sha, Lv, Ranbo, Duan, Desheng, Zhang, Wanhong, Guo, Yingchang, Wang, Pengfei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7388303/
https://www.ncbi.nlm.nih.gov/pubmed/32705233
http://dx.doi.org/10.3892/or.2020.7684
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author Chang, Keliang
Wang, Genwei
Lou, Jinfeng
Hao, Sha
Lv, Ranbo
Duan, Desheng
Zhang, Wanhong
Guo, Yingchang
Wang, Pengfei
author_facet Chang, Keliang
Wang, Genwei
Lou, Jinfeng
Hao, Sha
Lv, Ranbo
Duan, Desheng
Zhang, Wanhong
Guo, Yingchang
Wang, Pengfei
author_sort Chang, Keliang
collection PubMed
description Long non-coding RNAs (lncRNAs) contribute to the tumorigeneses of numerous types of cancer, including glioma. The present study was designed to unveil a novel lncRNA functioning in glioma and explore the underlying mechanisms. lncRNA titin-antisense RNA1 (TTN-AS1), miR-27b-3p and Runt-related transcription factor 1 (RUNX1) expression in glioma tissues and cell lines was estimated by RT-qPCR. Si-TTN-AS1 was transfected into glioma cell lines (U251 and LN229), and CCK-8 assay, flow cytometry, wound healing and Transwell assays were applied to estimate the function of TTN-AS1 in glioma cells. miR-27b-3p inhibitor was used to explore the mechanisms. The results revealed that TTN-AS1 was highly expressed in glioma specimens and cell lines. Downregulation of TTN-AS1 inhibited the proliferation, migration and invasion of the glioma cells, as well as increased the rate of apoptosis. In vivo, the tumor growth was also inhibited by TTN-AS1 depletion in nude mice. Furthermore, we revealed that TTN-AS1 exerted oncogenic effects via sponging miR-27b-3p and thereby positively regulating RUNX1 expression. In conclusion, the present study supported that TTN-AS1 acts as an oncogene in glioma by targeting miR-27b-3p to release RUNX1. This finding may contribute to gene therapy of glioma.
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spelling pubmed-73883032020-07-31 lncRNA TTN-AS1 upregulates RUNX1 to enhance glioma progression via sponging miR-27b-3p Chang, Keliang Wang, Genwei Lou, Jinfeng Hao, Sha Lv, Ranbo Duan, Desheng Zhang, Wanhong Guo, Yingchang Wang, Pengfei Oncol Rep Articles Long non-coding RNAs (lncRNAs) contribute to the tumorigeneses of numerous types of cancer, including glioma. The present study was designed to unveil a novel lncRNA functioning in glioma and explore the underlying mechanisms. lncRNA titin-antisense RNA1 (TTN-AS1), miR-27b-3p and Runt-related transcription factor 1 (RUNX1) expression in glioma tissues and cell lines was estimated by RT-qPCR. Si-TTN-AS1 was transfected into glioma cell lines (U251 and LN229), and CCK-8 assay, flow cytometry, wound healing and Transwell assays were applied to estimate the function of TTN-AS1 in glioma cells. miR-27b-3p inhibitor was used to explore the mechanisms. The results revealed that TTN-AS1 was highly expressed in glioma specimens and cell lines. Downregulation of TTN-AS1 inhibited the proliferation, migration and invasion of the glioma cells, as well as increased the rate of apoptosis. In vivo, the tumor growth was also inhibited by TTN-AS1 depletion in nude mice. Furthermore, we revealed that TTN-AS1 exerted oncogenic effects via sponging miR-27b-3p and thereby positively regulating RUNX1 expression. In conclusion, the present study supported that TTN-AS1 acts as an oncogene in glioma by targeting miR-27b-3p to release RUNX1. This finding may contribute to gene therapy of glioma. D.A. Spandidos 2020-09 2020-07-10 /pmc/articles/PMC7388303/ /pubmed/32705233 http://dx.doi.org/10.3892/or.2020.7684 Text en Copyright: © Chang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Chang, Keliang
Wang, Genwei
Lou, Jinfeng
Hao, Sha
Lv, Ranbo
Duan, Desheng
Zhang, Wanhong
Guo, Yingchang
Wang, Pengfei
lncRNA TTN-AS1 upregulates RUNX1 to enhance glioma progression via sponging miR-27b-3p
title lncRNA TTN-AS1 upregulates RUNX1 to enhance glioma progression via sponging miR-27b-3p
title_full lncRNA TTN-AS1 upregulates RUNX1 to enhance glioma progression via sponging miR-27b-3p
title_fullStr lncRNA TTN-AS1 upregulates RUNX1 to enhance glioma progression via sponging miR-27b-3p
title_full_unstemmed lncRNA TTN-AS1 upregulates RUNX1 to enhance glioma progression via sponging miR-27b-3p
title_short lncRNA TTN-AS1 upregulates RUNX1 to enhance glioma progression via sponging miR-27b-3p
title_sort lncrna ttn-as1 upregulates runx1 to enhance glioma progression via sponging mir-27b-3p
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7388303/
https://www.ncbi.nlm.nih.gov/pubmed/32705233
http://dx.doi.org/10.3892/or.2020.7684
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