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Composition and biosynthetic machinery of the Blumeria graminis f. sp. hordei conidia cell wall
Infection of barley with the powdery mildew causal agent, Blumeria graminis f. sp. hordei (Bgh), can lead to devastating damage to barley crops. The recent emergence of fungicide resistance imposes a need to develop new antifungal strategies. The enzymes involved in cell wall biosynthesis are ideal...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7388969/ https://www.ncbi.nlm.nih.gov/pubmed/32743145 http://dx.doi.org/10.1016/j.tcsw.2019.100029 |
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author | Pham, Trang A.T. Kyriacou, Bianca A. Schwerdt, Julian G. Shirley, Neil J. Xing, Xiaohui Bulone, Vincent Little, Alan |
author_facet | Pham, Trang A.T. Kyriacou, Bianca A. Schwerdt, Julian G. Shirley, Neil J. Xing, Xiaohui Bulone, Vincent Little, Alan |
author_sort | Pham, Trang A.T. |
collection | PubMed |
description | Infection of barley with the powdery mildew causal agent, Blumeria graminis f. sp. hordei (Bgh), can lead to devastating damage to barley crops. The recent emergence of fungicide resistance imposes a need to develop new antifungal strategies. The enzymes involved in cell wall biosynthesis are ideal targets for the development of fungicides. However, in order to narrow down any target proteins involved in cell wall formation, a greater understanding of the cell wall structure and composition is required. Here, we present a detailed carbohydrate analysis of the Bgh conidial cell wall, a full annotation of Carbohydrate Active enZymes (CAZy) in the Bgh genome, and a comprehensive expression profile of the genes involved in cell wall metabolism. Glycosidic linkage analysis has revealed that the cell wall polysaccharide fraction of Bgh conidia predominantly consists of glucosyl residues (63.1%) and has a greater proportion of galactopyranosyl residues compared to other species (8.5%). Trace amounts of xylosyl residues were also detected, which is unusual in ascomycetes. Transcripts of the genes involved in cell wall metabolism show high expression of chitin deacetylases, which assist fungi in evading the host defence system by deacetylating chitin to chitosan. The data presented suggest that the cell wall components of the conidia and the corresponding obligate biotrophic CAZy gene profile play a key role in the infection process. |
format | Online Article Text |
id | pubmed-7388969 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-73889692020-07-31 Composition and biosynthetic machinery of the Blumeria graminis f. sp. hordei conidia cell wall Pham, Trang A.T. Kyriacou, Bianca A. Schwerdt, Julian G. Shirley, Neil J. Xing, Xiaohui Bulone, Vincent Little, Alan Cell Surf Article Infection of barley with the powdery mildew causal agent, Blumeria graminis f. sp. hordei (Bgh), can lead to devastating damage to barley crops. The recent emergence of fungicide resistance imposes a need to develop new antifungal strategies. The enzymes involved in cell wall biosynthesis are ideal targets for the development of fungicides. However, in order to narrow down any target proteins involved in cell wall formation, a greater understanding of the cell wall structure and composition is required. Here, we present a detailed carbohydrate analysis of the Bgh conidial cell wall, a full annotation of Carbohydrate Active enZymes (CAZy) in the Bgh genome, and a comprehensive expression profile of the genes involved in cell wall metabolism. Glycosidic linkage analysis has revealed that the cell wall polysaccharide fraction of Bgh conidia predominantly consists of glucosyl residues (63.1%) and has a greater proportion of galactopyranosyl residues compared to other species (8.5%). Trace amounts of xylosyl residues were also detected, which is unusual in ascomycetes. Transcripts of the genes involved in cell wall metabolism show high expression of chitin deacetylases, which assist fungi in evading the host defence system by deacetylating chitin to chitosan. The data presented suggest that the cell wall components of the conidia and the corresponding obligate biotrophic CAZy gene profile play a key role in the infection process. Elsevier 2019-08-14 /pmc/articles/PMC7388969/ /pubmed/32743145 http://dx.doi.org/10.1016/j.tcsw.2019.100029 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pham, Trang A.T. Kyriacou, Bianca A. Schwerdt, Julian G. Shirley, Neil J. Xing, Xiaohui Bulone, Vincent Little, Alan Composition and biosynthetic machinery of the Blumeria graminis f. sp. hordei conidia cell wall |
title | Composition and biosynthetic machinery of the Blumeria graminis f. sp. hordei conidia cell wall |
title_full | Composition and biosynthetic machinery of the Blumeria graminis f. sp. hordei conidia cell wall |
title_fullStr | Composition and biosynthetic machinery of the Blumeria graminis f. sp. hordei conidia cell wall |
title_full_unstemmed | Composition and biosynthetic machinery of the Blumeria graminis f. sp. hordei conidia cell wall |
title_short | Composition and biosynthetic machinery of the Blumeria graminis f. sp. hordei conidia cell wall |
title_sort | composition and biosynthetic machinery of the blumeria graminis f. sp. hordei conidia cell wall |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7388969/ https://www.ncbi.nlm.nih.gov/pubmed/32743145 http://dx.doi.org/10.1016/j.tcsw.2019.100029 |
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