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Plasma microRNAs biomarkers in mild cognitive impairment among patients with type 2 diabetes mellitus

OBJECTIVES: To assess the potential value of some miRNAs as diagnostic biomarkers for mild cognitive impairment (MCI) among patients with type2 diabetes mellitus (T2DM) and to identify other risk factors for MCI among them. METHODS: This study enrolled 163 adults with T2DM using face to face intervi...

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Autores principales: Salama, Iman I., Sami, Samia M., Abdellatif, Ghada A., Mohsen, Amira, Rasmy, Hanaa, Kamel, Solaf Ahmed, Ibrahim, Mona Hamed, Mostafa, Mona, Fouad, Walaa A., Raslan, Hala M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7390351/
https://www.ncbi.nlm.nih.gov/pubmed/32726329
http://dx.doi.org/10.1371/journal.pone.0236453
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author Salama, Iman I.
Sami, Samia M.
Abdellatif, Ghada A.
Mohsen, Amira
Rasmy, Hanaa
Kamel, Solaf Ahmed
Ibrahim, Mona Hamed
Mostafa, Mona
Fouad, Walaa A.
Raslan, Hala M.
author_facet Salama, Iman I.
Sami, Samia M.
Abdellatif, Ghada A.
Mohsen, Amira
Rasmy, Hanaa
Kamel, Solaf Ahmed
Ibrahim, Mona Hamed
Mostafa, Mona
Fouad, Walaa A.
Raslan, Hala M.
author_sort Salama, Iman I.
collection PubMed
description OBJECTIVES: To assess the potential value of some miRNAs as diagnostic biomarkers for mild cognitive impairment (MCI) among patients with type2 diabetes mellitus (T2DM) and to identify other risk factors for MCI among them. METHODS: This study enrolled 163 adults with T2DM using face to face interview. Cognitive function with its domains was assessed using Adenbrooke’s Cognitive Examination III (ACE III). Lipid profile, glycated hemoglobin, and miR-128, miR-132, miR- 874, miR-134, miR-323, and miR-382 expressions, using quantitative real-time PCR, were assessed. RESULTS: MCI was detected among 59/163 (36.2%) patients with T2DM. Plasma expression of miR-132 was significantly higher in T2DM patients with MCI compared to those without MCI and to normal cognitive healthy individuals (median = 2, 1.1 and 1.2 respectively, P < 0.05. Logistic regression analysis showed that higher miR-132 expression with adjusted odds ratio (AOR): 1.2 (95% CI 1.0–1.3), female gender (AOR:2.1; 95%CI 1.0–4.3), education below postgraduate (secondary and university education with AOR: 9.5 & 19.4 respectively) were the significant predicting factors for MCI among T2DM patients. Using ROC curve, miR-132 was the only assayed miRNA that significantly differentiates T2DM patients with MCI from those with normal cognition with 72.3% sensitivity, 56.2% specificity, and 63.8% accuracy (P < 0.05). Other studied miRNAs showed lower sensitivity and specificity for detecting MCI among studied T2DM participants. CONCLUSION: MCI affects nearly one-third of adult patients with T2DM. A significantly over expression of miR-132 was detected among T2DM with MCI compared to those with normal cognition.
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spelling pubmed-73903512020-08-05 Plasma microRNAs biomarkers in mild cognitive impairment among patients with type 2 diabetes mellitus Salama, Iman I. Sami, Samia M. Abdellatif, Ghada A. Mohsen, Amira Rasmy, Hanaa Kamel, Solaf Ahmed Ibrahim, Mona Hamed Mostafa, Mona Fouad, Walaa A. Raslan, Hala M. PLoS One Research Article OBJECTIVES: To assess the potential value of some miRNAs as diagnostic biomarkers for mild cognitive impairment (MCI) among patients with type2 diabetes mellitus (T2DM) and to identify other risk factors for MCI among them. METHODS: This study enrolled 163 adults with T2DM using face to face interview. Cognitive function with its domains was assessed using Adenbrooke’s Cognitive Examination III (ACE III). Lipid profile, glycated hemoglobin, and miR-128, miR-132, miR- 874, miR-134, miR-323, and miR-382 expressions, using quantitative real-time PCR, were assessed. RESULTS: MCI was detected among 59/163 (36.2%) patients with T2DM. Plasma expression of miR-132 was significantly higher in T2DM patients with MCI compared to those without MCI and to normal cognitive healthy individuals (median = 2, 1.1 and 1.2 respectively, P < 0.05. Logistic regression analysis showed that higher miR-132 expression with adjusted odds ratio (AOR): 1.2 (95% CI 1.0–1.3), female gender (AOR:2.1; 95%CI 1.0–4.3), education below postgraduate (secondary and university education with AOR: 9.5 & 19.4 respectively) were the significant predicting factors for MCI among T2DM patients. Using ROC curve, miR-132 was the only assayed miRNA that significantly differentiates T2DM patients with MCI from those with normal cognition with 72.3% sensitivity, 56.2% specificity, and 63.8% accuracy (P < 0.05). Other studied miRNAs showed lower sensitivity and specificity for detecting MCI among studied T2DM participants. CONCLUSION: MCI affects nearly one-third of adult patients with T2DM. A significantly over expression of miR-132 was detected among T2DM with MCI compared to those with normal cognition. Public Library of Science 2020-07-29 /pmc/articles/PMC7390351/ /pubmed/32726329 http://dx.doi.org/10.1371/journal.pone.0236453 Text en © 2020 Salama et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Salama, Iman I.
Sami, Samia M.
Abdellatif, Ghada A.
Mohsen, Amira
Rasmy, Hanaa
Kamel, Solaf Ahmed
Ibrahim, Mona Hamed
Mostafa, Mona
Fouad, Walaa A.
Raslan, Hala M.
Plasma microRNAs biomarkers in mild cognitive impairment among patients with type 2 diabetes mellitus
title Plasma microRNAs biomarkers in mild cognitive impairment among patients with type 2 diabetes mellitus
title_full Plasma microRNAs biomarkers in mild cognitive impairment among patients with type 2 diabetes mellitus
title_fullStr Plasma microRNAs biomarkers in mild cognitive impairment among patients with type 2 diabetes mellitus
title_full_unstemmed Plasma microRNAs biomarkers in mild cognitive impairment among patients with type 2 diabetes mellitus
title_short Plasma microRNAs biomarkers in mild cognitive impairment among patients with type 2 diabetes mellitus
title_sort plasma micrornas biomarkers in mild cognitive impairment among patients with type 2 diabetes mellitus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7390351/
https://www.ncbi.nlm.nih.gov/pubmed/32726329
http://dx.doi.org/10.1371/journal.pone.0236453
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