An Optimized Protocol for Human Direct Cardiac Reprogramming

Direct cardiac reprogramming, the conversion of fibroblasts into cardiomyocyte-like cells (iCMs), is an attractive approach to heal the injured heart. Here we present a new approach to human cardiac reprogramming that utilizes a polycistronic three-factor reprogramming cocktail and one microRNA. Our...

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Detalles Bibliográficos
Autores principales: Garbutt, Tiffany A., Zhou, Yang, Keepers, Benjamin, Liu, Jiandong, Qian, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7390466/
https://www.ncbi.nlm.nih.gov/pubmed/32728671
http://dx.doi.org/10.1016/j.xpro.2019.100010
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author Garbutt, Tiffany A.
Zhou, Yang
Keepers, Benjamin
Liu, Jiandong
Qian, Li
author_facet Garbutt, Tiffany A.
Zhou, Yang
Keepers, Benjamin
Liu, Jiandong
Qian, Li
author_sort Garbutt, Tiffany A.
collection PubMed
description Direct cardiac reprogramming, the conversion of fibroblasts into cardiomyocyte-like cells (iCMs), is an attractive approach to heal the injured heart. Here we present a new approach to human cardiac reprogramming that utilizes a polycistronic three-factor reprogramming cocktail and one microRNA. Our protocol produces cardiac Troponin T positive human iCMs (hiCMs) at an efficiency of 40%–60%, approximately double that of previous protocols, within just 2 weeks. The resulting hiCMs display cardiomyocyte-like sarcomere structure, gene expression, and calcium oscillation. For complete details on the use and execution of this protocol, please refer to Zhou et al. (2019).
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spelling pubmed-73904662020-07-29 An Optimized Protocol for Human Direct Cardiac Reprogramming Garbutt, Tiffany A. Zhou, Yang Keepers, Benjamin Liu, Jiandong Qian, Li STAR Protoc Protocol Direct cardiac reprogramming, the conversion of fibroblasts into cardiomyocyte-like cells (iCMs), is an attractive approach to heal the injured heart. Here we present a new approach to human cardiac reprogramming that utilizes a polycistronic three-factor reprogramming cocktail and one microRNA. Our protocol produces cardiac Troponin T positive human iCMs (hiCMs) at an efficiency of 40%–60%, approximately double that of previous protocols, within just 2 weeks. The resulting hiCMs display cardiomyocyte-like sarcomere structure, gene expression, and calcium oscillation. For complete details on the use and execution of this protocol, please refer to Zhou et al. (2019). Elsevier 2020-06-03 /pmc/articles/PMC7390466/ /pubmed/32728671 http://dx.doi.org/10.1016/j.xpro.2019.100010 Text en © 2020. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Garbutt, Tiffany A.
Zhou, Yang
Keepers, Benjamin
Liu, Jiandong
Qian, Li
An Optimized Protocol for Human Direct Cardiac Reprogramming
title An Optimized Protocol for Human Direct Cardiac Reprogramming
title_full An Optimized Protocol for Human Direct Cardiac Reprogramming
title_fullStr An Optimized Protocol for Human Direct Cardiac Reprogramming
title_full_unstemmed An Optimized Protocol for Human Direct Cardiac Reprogramming
title_short An Optimized Protocol for Human Direct Cardiac Reprogramming
title_sort optimized protocol for human direct cardiac reprogramming
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7390466/
https://www.ncbi.nlm.nih.gov/pubmed/32728671
http://dx.doi.org/10.1016/j.xpro.2019.100010
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