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Bacterial Blight Induced Shifts in Endophytic Microbiome of Rice Leaves and the Enrichment of Specific Bacterial Strains With Pathogen Antagonism

The endophytic microbiome plays an important role in plant health and pathogenesis. However, little is known about its relationship with bacterial blight (BB) of rice caused by Xanthomonas oryzae pv. oryzae (Xoo). The current study compared the community compositional structure of the endophytic mic...

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Autores principales: Yang, Fenghuan, Zhang, Jie, Zhang, Huaying, Ji, Guanghai, Zeng, Liexian, Li, Yan, Yu, Chao, Fernando, W. G. Dilantha, Chen, Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7390967/
https://www.ncbi.nlm.nih.gov/pubmed/32793250
http://dx.doi.org/10.3389/fpls.2020.00963
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author Yang, Fenghuan
Zhang, Jie
Zhang, Huaying
Ji, Guanghai
Zeng, Liexian
Li, Yan
Yu, Chao
Fernando, W. G. Dilantha
Chen, Wen
author_facet Yang, Fenghuan
Zhang, Jie
Zhang, Huaying
Ji, Guanghai
Zeng, Liexian
Li, Yan
Yu, Chao
Fernando, W. G. Dilantha
Chen, Wen
author_sort Yang, Fenghuan
collection PubMed
description The endophytic microbiome plays an important role in plant health and pathogenesis. However, little is known about its relationship with bacterial blight (BB) of rice caused by Xanthomonas oryzae pv. oryzae (Xoo). The current study compared the community compositional structure of the endophytic microbiota in healthy and BB symptomatic leaves of rice through a metabarcoding approach, which revealed BB induced a decrease in the alpha-diversity of the fungal communities and an increase in the bacterial communities. BB-diseased rice leaves were enriched with saprophytic fungi that are capable of decomposing plant cell walls (e.g. Khuskia spp. and Leptosphaerulina spp.), while healthy rice leaves were found to be significantly more abundant with plant pathogens or mycotoxin-producing fungi (e.g. Fusarium, Magnaporthe, and Aspergillus). The endophytic bacterial communities of BB-diseased leaves were significantly enriched with Pantoea, Pseudomonas, and Curtobacterium, strains. Pantoea sp. isolates from BB leaves are identified as promising candidates for the biocontrol of BB for their ability to inhibit in vitro growth of Xoo, suppress the development of rice BB disease, and possess multiple PGP characteristics. Our study revealed BB-induced complexed changes in the endophytic fungal and bacterial communities of rice leaves and demonstrated that BB-associated enrichment of some endophytic bacterial taxa, e.g. Pantoea sp. isolates, may play important roles in suppressing the development of BB disease in rice.
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spelling pubmed-73909672020-08-12 Bacterial Blight Induced Shifts in Endophytic Microbiome of Rice Leaves and the Enrichment of Specific Bacterial Strains With Pathogen Antagonism Yang, Fenghuan Zhang, Jie Zhang, Huaying Ji, Guanghai Zeng, Liexian Li, Yan Yu, Chao Fernando, W. G. Dilantha Chen, Wen Front Plant Sci Plant Science The endophytic microbiome plays an important role in plant health and pathogenesis. However, little is known about its relationship with bacterial blight (BB) of rice caused by Xanthomonas oryzae pv. oryzae (Xoo). The current study compared the community compositional structure of the endophytic microbiota in healthy and BB symptomatic leaves of rice through a metabarcoding approach, which revealed BB induced a decrease in the alpha-diversity of the fungal communities and an increase in the bacterial communities. BB-diseased rice leaves were enriched with saprophytic fungi that are capable of decomposing plant cell walls (e.g. Khuskia spp. and Leptosphaerulina spp.), while healthy rice leaves were found to be significantly more abundant with plant pathogens or mycotoxin-producing fungi (e.g. Fusarium, Magnaporthe, and Aspergillus). The endophytic bacterial communities of BB-diseased leaves were significantly enriched with Pantoea, Pseudomonas, and Curtobacterium, strains. Pantoea sp. isolates from BB leaves are identified as promising candidates for the biocontrol of BB for their ability to inhibit in vitro growth of Xoo, suppress the development of rice BB disease, and possess multiple PGP characteristics. Our study revealed BB-induced complexed changes in the endophytic fungal and bacterial communities of rice leaves and demonstrated that BB-associated enrichment of some endophytic bacterial taxa, e.g. Pantoea sp. isolates, may play important roles in suppressing the development of BB disease in rice. Frontiers Media S.A. 2020-07-23 /pmc/articles/PMC7390967/ /pubmed/32793250 http://dx.doi.org/10.3389/fpls.2020.00963 Text en Copyright © 2020 Yang, Zhang, Zhang, Ji, Zeng, Li, Yu, Fernando and Her Majesty the Queen in Right of Canada, as represented by the Minister of Agriculture and Agri-Food Canada http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Yang, Fenghuan
Zhang, Jie
Zhang, Huaying
Ji, Guanghai
Zeng, Liexian
Li, Yan
Yu, Chao
Fernando, W. G. Dilantha
Chen, Wen
Bacterial Blight Induced Shifts in Endophytic Microbiome of Rice Leaves and the Enrichment of Specific Bacterial Strains With Pathogen Antagonism
title Bacterial Blight Induced Shifts in Endophytic Microbiome of Rice Leaves and the Enrichment of Specific Bacterial Strains With Pathogen Antagonism
title_full Bacterial Blight Induced Shifts in Endophytic Microbiome of Rice Leaves and the Enrichment of Specific Bacterial Strains With Pathogen Antagonism
title_fullStr Bacterial Blight Induced Shifts in Endophytic Microbiome of Rice Leaves and the Enrichment of Specific Bacterial Strains With Pathogen Antagonism
title_full_unstemmed Bacterial Blight Induced Shifts in Endophytic Microbiome of Rice Leaves and the Enrichment of Specific Bacterial Strains With Pathogen Antagonism
title_short Bacterial Blight Induced Shifts in Endophytic Microbiome of Rice Leaves and the Enrichment of Specific Bacterial Strains With Pathogen Antagonism
title_sort bacterial blight induced shifts in endophytic microbiome of rice leaves and the enrichment of specific bacterial strains with pathogen antagonism
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7390967/
https://www.ncbi.nlm.nih.gov/pubmed/32793250
http://dx.doi.org/10.3389/fpls.2020.00963
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