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Serum Exosomal miR-941 as a promising Oncogenic Biomarker for Laryngeal Squamous Cell Carcinoma

At present, no blood-based biomarkers have been used in clinical practice for laryngeal squamous cell carcinoma (LSCC). Increasing evidence suggests that circulating exosomal microRNAs (miRNAs) may serve as potential diagnostic biomarkers for various cancers. This study aims to identify and evaluate...

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Autores principales: Zhao, Qinli, Zheng, Xiwang, Guo, Huina, Xue, Xuting, Zhang, Yuliang, Niu, Min, Cui, Jiajia, Liu, Hongliang, Luo, Hongjie, Yang, Dongli, Shi, Yong, Huangfu, Hui, Wen, Shuxin, Wu, Yongyan, Gao, Wei, Wang, Binquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7391210/
https://www.ncbi.nlm.nih.gov/pubmed/32742479
http://dx.doi.org/10.7150/jca.45394
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author Zhao, Qinli
Zheng, Xiwang
Guo, Huina
Xue, Xuting
Zhang, Yuliang
Niu, Min
Cui, Jiajia
Liu, Hongliang
Luo, Hongjie
Yang, Dongli
Shi, Yong
Huangfu, Hui
Wen, Shuxin
Wu, Yongyan
Gao, Wei
Wang, Binquan
author_facet Zhao, Qinli
Zheng, Xiwang
Guo, Huina
Xue, Xuting
Zhang, Yuliang
Niu, Min
Cui, Jiajia
Liu, Hongliang
Luo, Hongjie
Yang, Dongli
Shi, Yong
Huangfu, Hui
Wen, Shuxin
Wu, Yongyan
Gao, Wei
Wang, Binquan
author_sort Zhao, Qinli
collection PubMed
description At present, no blood-based biomarkers have been used in clinical practice for laryngeal squamous cell carcinoma (LSCC). Increasing evidence suggests that circulating exosomal microRNAs (miRNAs) may serve as potential diagnostic biomarkers for various cancers. This study aims to identify and evaluate serum exosomal miRNAs for LSCC diagnosis. The ExoQuick solution (EQ), which provides a high-yield and is a highly efficient exosome isolation method, was selected to isolate serum exosomes in the current study. In LSCC samples, exosome concentrations were higher than in healthy control (HC) samples. RNA-seq analysis identified a total of 1608 miRNAs, with 34 upregulated and 41 downregulated in LSCC samples relative to HC samples. Furthermore, qRT-PCR showed that miR-941 is significantly upregulated in LSCC serum exosomes, with this same trend seen in LSCC tissues and cells. Moreover, when examining miR-941 in cell lines, miR-941 overexpression promoted proliferation and invasion, while miR-941 knockdown inhibited cell proliferation and invasion. ROC curve analysis showed that miR-941 has an area under the curve (AUC) of 0.797 (95% CI = 0.676-0.918) for distinguishing LSCC patients from HCs. In conclusion, serum exosomal miR-941 may serve as a promising oncogenic biomarker for diagnosing LSCC, and has the potential as a therapeutic target.
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spelling pubmed-73912102020-07-31 Serum Exosomal miR-941 as a promising Oncogenic Biomarker for Laryngeal Squamous Cell Carcinoma Zhao, Qinli Zheng, Xiwang Guo, Huina Xue, Xuting Zhang, Yuliang Niu, Min Cui, Jiajia Liu, Hongliang Luo, Hongjie Yang, Dongli Shi, Yong Huangfu, Hui Wen, Shuxin Wu, Yongyan Gao, Wei Wang, Binquan J Cancer Research Paper At present, no blood-based biomarkers have been used in clinical practice for laryngeal squamous cell carcinoma (LSCC). Increasing evidence suggests that circulating exosomal microRNAs (miRNAs) may serve as potential diagnostic biomarkers for various cancers. This study aims to identify and evaluate serum exosomal miRNAs for LSCC diagnosis. The ExoQuick solution (EQ), which provides a high-yield and is a highly efficient exosome isolation method, was selected to isolate serum exosomes in the current study. In LSCC samples, exosome concentrations were higher than in healthy control (HC) samples. RNA-seq analysis identified a total of 1608 miRNAs, with 34 upregulated and 41 downregulated in LSCC samples relative to HC samples. Furthermore, qRT-PCR showed that miR-941 is significantly upregulated in LSCC serum exosomes, with this same trend seen in LSCC tissues and cells. Moreover, when examining miR-941 in cell lines, miR-941 overexpression promoted proliferation and invasion, while miR-941 knockdown inhibited cell proliferation and invasion. ROC curve analysis showed that miR-941 has an area under the curve (AUC) of 0.797 (95% CI = 0.676-0.918) for distinguishing LSCC patients from HCs. In conclusion, serum exosomal miR-941 may serve as a promising oncogenic biomarker for diagnosing LSCC, and has the potential as a therapeutic target. Ivyspring International Publisher 2020-07-09 /pmc/articles/PMC7391210/ /pubmed/32742479 http://dx.doi.org/10.7150/jca.45394 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Zhao, Qinli
Zheng, Xiwang
Guo, Huina
Xue, Xuting
Zhang, Yuliang
Niu, Min
Cui, Jiajia
Liu, Hongliang
Luo, Hongjie
Yang, Dongli
Shi, Yong
Huangfu, Hui
Wen, Shuxin
Wu, Yongyan
Gao, Wei
Wang, Binquan
Serum Exosomal miR-941 as a promising Oncogenic Biomarker for Laryngeal Squamous Cell Carcinoma
title Serum Exosomal miR-941 as a promising Oncogenic Biomarker for Laryngeal Squamous Cell Carcinoma
title_full Serum Exosomal miR-941 as a promising Oncogenic Biomarker for Laryngeal Squamous Cell Carcinoma
title_fullStr Serum Exosomal miR-941 as a promising Oncogenic Biomarker for Laryngeal Squamous Cell Carcinoma
title_full_unstemmed Serum Exosomal miR-941 as a promising Oncogenic Biomarker for Laryngeal Squamous Cell Carcinoma
title_short Serum Exosomal miR-941 as a promising Oncogenic Biomarker for Laryngeal Squamous Cell Carcinoma
title_sort serum exosomal mir-941 as a promising oncogenic biomarker for laryngeal squamous cell carcinoma
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7391210/
https://www.ncbi.nlm.nih.gov/pubmed/32742479
http://dx.doi.org/10.7150/jca.45394
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