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Serum Exosomal miR-941 as a promising Oncogenic Biomarker for Laryngeal Squamous Cell Carcinoma
At present, no blood-based biomarkers have been used in clinical practice for laryngeal squamous cell carcinoma (LSCC). Increasing evidence suggests that circulating exosomal microRNAs (miRNAs) may serve as potential diagnostic biomarkers for various cancers. This study aims to identify and evaluate...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7391210/ https://www.ncbi.nlm.nih.gov/pubmed/32742479 http://dx.doi.org/10.7150/jca.45394 |
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author | Zhao, Qinli Zheng, Xiwang Guo, Huina Xue, Xuting Zhang, Yuliang Niu, Min Cui, Jiajia Liu, Hongliang Luo, Hongjie Yang, Dongli Shi, Yong Huangfu, Hui Wen, Shuxin Wu, Yongyan Gao, Wei Wang, Binquan |
author_facet | Zhao, Qinli Zheng, Xiwang Guo, Huina Xue, Xuting Zhang, Yuliang Niu, Min Cui, Jiajia Liu, Hongliang Luo, Hongjie Yang, Dongli Shi, Yong Huangfu, Hui Wen, Shuxin Wu, Yongyan Gao, Wei Wang, Binquan |
author_sort | Zhao, Qinli |
collection | PubMed |
description | At present, no blood-based biomarkers have been used in clinical practice for laryngeal squamous cell carcinoma (LSCC). Increasing evidence suggests that circulating exosomal microRNAs (miRNAs) may serve as potential diagnostic biomarkers for various cancers. This study aims to identify and evaluate serum exosomal miRNAs for LSCC diagnosis. The ExoQuick solution (EQ), which provides a high-yield and is a highly efficient exosome isolation method, was selected to isolate serum exosomes in the current study. In LSCC samples, exosome concentrations were higher than in healthy control (HC) samples. RNA-seq analysis identified a total of 1608 miRNAs, with 34 upregulated and 41 downregulated in LSCC samples relative to HC samples. Furthermore, qRT-PCR showed that miR-941 is significantly upregulated in LSCC serum exosomes, with this same trend seen in LSCC tissues and cells. Moreover, when examining miR-941 in cell lines, miR-941 overexpression promoted proliferation and invasion, while miR-941 knockdown inhibited cell proliferation and invasion. ROC curve analysis showed that miR-941 has an area under the curve (AUC) of 0.797 (95% CI = 0.676-0.918) for distinguishing LSCC patients from HCs. In conclusion, serum exosomal miR-941 may serve as a promising oncogenic biomarker for diagnosing LSCC, and has the potential as a therapeutic target. |
format | Online Article Text |
id | pubmed-7391210 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-73912102020-07-31 Serum Exosomal miR-941 as a promising Oncogenic Biomarker for Laryngeal Squamous Cell Carcinoma Zhao, Qinli Zheng, Xiwang Guo, Huina Xue, Xuting Zhang, Yuliang Niu, Min Cui, Jiajia Liu, Hongliang Luo, Hongjie Yang, Dongli Shi, Yong Huangfu, Hui Wen, Shuxin Wu, Yongyan Gao, Wei Wang, Binquan J Cancer Research Paper At present, no blood-based biomarkers have been used in clinical practice for laryngeal squamous cell carcinoma (LSCC). Increasing evidence suggests that circulating exosomal microRNAs (miRNAs) may serve as potential diagnostic biomarkers for various cancers. This study aims to identify and evaluate serum exosomal miRNAs for LSCC diagnosis. The ExoQuick solution (EQ), which provides a high-yield and is a highly efficient exosome isolation method, was selected to isolate serum exosomes in the current study. In LSCC samples, exosome concentrations were higher than in healthy control (HC) samples. RNA-seq analysis identified a total of 1608 miRNAs, with 34 upregulated and 41 downregulated in LSCC samples relative to HC samples. Furthermore, qRT-PCR showed that miR-941 is significantly upregulated in LSCC serum exosomes, with this same trend seen in LSCC tissues and cells. Moreover, when examining miR-941 in cell lines, miR-941 overexpression promoted proliferation and invasion, while miR-941 knockdown inhibited cell proliferation and invasion. ROC curve analysis showed that miR-941 has an area under the curve (AUC) of 0.797 (95% CI = 0.676-0.918) for distinguishing LSCC patients from HCs. In conclusion, serum exosomal miR-941 may serve as a promising oncogenic biomarker for diagnosing LSCC, and has the potential as a therapeutic target. Ivyspring International Publisher 2020-07-09 /pmc/articles/PMC7391210/ /pubmed/32742479 http://dx.doi.org/10.7150/jca.45394 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Zhao, Qinli Zheng, Xiwang Guo, Huina Xue, Xuting Zhang, Yuliang Niu, Min Cui, Jiajia Liu, Hongliang Luo, Hongjie Yang, Dongli Shi, Yong Huangfu, Hui Wen, Shuxin Wu, Yongyan Gao, Wei Wang, Binquan Serum Exosomal miR-941 as a promising Oncogenic Biomarker for Laryngeal Squamous Cell Carcinoma |
title | Serum Exosomal miR-941 as a promising Oncogenic Biomarker for Laryngeal Squamous Cell Carcinoma |
title_full | Serum Exosomal miR-941 as a promising Oncogenic Biomarker for Laryngeal Squamous Cell Carcinoma |
title_fullStr | Serum Exosomal miR-941 as a promising Oncogenic Biomarker for Laryngeal Squamous Cell Carcinoma |
title_full_unstemmed | Serum Exosomal miR-941 as a promising Oncogenic Biomarker for Laryngeal Squamous Cell Carcinoma |
title_short | Serum Exosomal miR-941 as a promising Oncogenic Biomarker for Laryngeal Squamous Cell Carcinoma |
title_sort | serum exosomal mir-941 as a promising oncogenic biomarker for laryngeal squamous cell carcinoma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7391210/ https://www.ncbi.nlm.nih.gov/pubmed/32742479 http://dx.doi.org/10.7150/jca.45394 |
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