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Development of an ic-ELISA and Immunochromatographic Strip Assay for the Detection of Diacetoxyscirpenol in Rice

[Image: see text] Diacetoxyscirpenol (DAS) is a highly toxic type A trichothecene, a natural contaminant in food and animal feed, which is a serious hazard to human and animal health. An anti-DAS mAb, 3H10, with high sensitivity and specificity, was prepared, and an indirect competitive enzyme-linke...

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Autores principales: Jin, Guohao, Wu, Xiaoling, Cui, Gang, Liu, Liqiang, Kuang, Hua, Xu, Chuanlai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7391247/
https://www.ncbi.nlm.nih.gov/pubmed/32743158
http://dx.doi.org/10.1021/acsomega.9b02496
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author Jin, Guohao
Wu, Xiaoling
Cui, Gang
Liu, Liqiang
Kuang, Hua
Xu, Chuanlai
author_facet Jin, Guohao
Wu, Xiaoling
Cui, Gang
Liu, Liqiang
Kuang, Hua
Xu, Chuanlai
author_sort Jin, Guohao
collection PubMed
description [Image: see text] Diacetoxyscirpenol (DAS) is a highly toxic type A trichothecene, a natural contaminant in food and animal feed, which is a serious hazard to human and animal health. An anti-DAS mAb, 3H10, with high sensitivity and specificity, was prepared, and an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a lateral-flow immunochromatographic strip (ICA strip) were developed for rapid testing of DAS in rice samples. The 50% inhibitory concentration and limit of detection of ic-ELISA were 5.97 and 0.78 ng/mL, respectively. The recovery of rice samples ranged from 99.4 to 110.7%, demonstrating that the analytical method can be used to detect rice samples. The cutoff value of the lateral-flow ICA strip to DAS was 500 ng/g. The developed immunoassay method can provide an effective method of initially detecting and screening DAS in food and feed samples.
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spelling pubmed-73912472020-07-31 Development of an ic-ELISA and Immunochromatographic Strip Assay for the Detection of Diacetoxyscirpenol in Rice Jin, Guohao Wu, Xiaoling Cui, Gang Liu, Liqiang Kuang, Hua Xu, Chuanlai ACS Omega [Image: see text] Diacetoxyscirpenol (DAS) is a highly toxic type A trichothecene, a natural contaminant in food and animal feed, which is a serious hazard to human and animal health. An anti-DAS mAb, 3H10, with high sensitivity and specificity, was prepared, and an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a lateral-flow immunochromatographic strip (ICA strip) were developed for rapid testing of DAS in rice samples. The 50% inhibitory concentration and limit of detection of ic-ELISA were 5.97 and 0.78 ng/mL, respectively. The recovery of rice samples ranged from 99.4 to 110.7%, demonstrating that the analytical method can be used to detect rice samples. The cutoff value of the lateral-flow ICA strip to DAS was 500 ng/g. The developed immunoassay method can provide an effective method of initially detecting and screening DAS in food and feed samples. American Chemical Society 2020-07-14 /pmc/articles/PMC7391247/ /pubmed/32743158 http://dx.doi.org/10.1021/acsomega.9b02496 Text en Copyright © 2020 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Jin, Guohao
Wu, Xiaoling
Cui, Gang
Liu, Liqiang
Kuang, Hua
Xu, Chuanlai
Development of an ic-ELISA and Immunochromatographic Strip Assay for the Detection of Diacetoxyscirpenol in Rice
title Development of an ic-ELISA and Immunochromatographic Strip Assay for the Detection of Diacetoxyscirpenol in Rice
title_full Development of an ic-ELISA and Immunochromatographic Strip Assay for the Detection of Diacetoxyscirpenol in Rice
title_fullStr Development of an ic-ELISA and Immunochromatographic Strip Assay for the Detection of Diacetoxyscirpenol in Rice
title_full_unstemmed Development of an ic-ELISA and Immunochromatographic Strip Assay for the Detection of Diacetoxyscirpenol in Rice
title_short Development of an ic-ELISA and Immunochromatographic Strip Assay for the Detection of Diacetoxyscirpenol in Rice
title_sort development of an ic-elisa and immunochromatographic strip assay for the detection of diacetoxyscirpenol in rice
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7391247/
https://www.ncbi.nlm.nih.gov/pubmed/32743158
http://dx.doi.org/10.1021/acsomega.9b02496
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