Cargando…

OsPAL2-1 Mediates Allelopathic Interactions Between Rice and Specific Microorganisms in the Rhizosphere Ecosystem

The use of plant allelopathy to control weeds in the field has been generally recognized as a win-win strategy because it is an environmentally friendly and resource-saving method. The mechanism of this natural weed-control method relies on allelochemicals, the rhizosphere microbiome, and their bio-...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Yingzhe, Jian, Xin, Li, Yue, Zeng, Xiaomei, Xu, Lining, Khan, Muhammad Umar, Lin, Wenxiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7391800/
https://www.ncbi.nlm.nih.gov/pubmed/32793125
http://dx.doi.org/10.3389/fmicb.2020.01411
_version_ 1783564725039136768
author Li, Yingzhe
Jian, Xin
Li, Yue
Zeng, Xiaomei
Xu, Lining
Khan, Muhammad Umar
Lin, Wenxiong
author_facet Li, Yingzhe
Jian, Xin
Li, Yue
Zeng, Xiaomei
Xu, Lining
Khan, Muhammad Umar
Lin, Wenxiong
author_sort Li, Yingzhe
collection PubMed
description The use of plant allelopathy to control weeds in the field has been generally recognized as a win-win strategy because it is an environmentally friendly and resource-saving method. The mechanism of this natural weed-control method relies on allelochemicals, the rhizosphere microbiome, and their bio-interaction, and exploring the link between allelochemicals and specific microbes helps accelerate the application of allelopathic characteristics in farming. In this study, we used allelopathic rice PI312777 (PI), its genetically modified OsPAL2-1 repression (PR) or overexpression (PO) lines, and non-allelopathic rice Lemont (Le) as donor plants to reveal the bio-interaction between rice allelochemicals and rhizosphere specific microorganisms. The results showed a higher content of phenolic acid exudation from the roots of PI than those of Le, which resulted in a significantly increased population of Myxococcus in the rhizosphere soil. Transgenic PO lines exhibited increasing exudation of phenolic acid, which led to the population of Myxococcus xanthus in the rhizosphere soil of PO to be significantly increased, while PR showed the opposite result in comparison with wild type PI. Exogenous application of phenolic acid induced the growth of M. xanthus, and the expressions of chemotaxis-related genes were up-regulated in M. xanthus. In addition, quercetin was identified in the culture medium; according to the bioassay determination, a quercetin concentration of 0.53 mM inhibited the root length by 60.59%. Our study indicates that OsPAL2-1 is among the efficient genes that regulate rice allelopathy by controlling the synthesis of phenolic acid allelochemicals, and phenolic acid (ferulic acid, FA) induces the chemotactic aggregation of M. xanthus, which promoted the proliferation and aggregation of this microbe. The potential allelochemical, quercetin was generated from the FA-induced M. xanthus cultured medium.
format Online
Article
Text
id pubmed-7391800
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-73918002020-08-12 OsPAL2-1 Mediates Allelopathic Interactions Between Rice and Specific Microorganisms in the Rhizosphere Ecosystem Li, Yingzhe Jian, Xin Li, Yue Zeng, Xiaomei Xu, Lining Khan, Muhammad Umar Lin, Wenxiong Front Microbiol Microbiology The use of plant allelopathy to control weeds in the field has been generally recognized as a win-win strategy because it is an environmentally friendly and resource-saving method. The mechanism of this natural weed-control method relies on allelochemicals, the rhizosphere microbiome, and their bio-interaction, and exploring the link between allelochemicals and specific microbes helps accelerate the application of allelopathic characteristics in farming. In this study, we used allelopathic rice PI312777 (PI), its genetically modified OsPAL2-1 repression (PR) or overexpression (PO) lines, and non-allelopathic rice Lemont (Le) as donor plants to reveal the bio-interaction between rice allelochemicals and rhizosphere specific microorganisms. The results showed a higher content of phenolic acid exudation from the roots of PI than those of Le, which resulted in a significantly increased population of Myxococcus in the rhizosphere soil. Transgenic PO lines exhibited increasing exudation of phenolic acid, which led to the population of Myxococcus xanthus in the rhizosphere soil of PO to be significantly increased, while PR showed the opposite result in comparison with wild type PI. Exogenous application of phenolic acid induced the growth of M. xanthus, and the expressions of chemotaxis-related genes were up-regulated in M. xanthus. In addition, quercetin was identified in the culture medium; according to the bioassay determination, a quercetin concentration of 0.53 mM inhibited the root length by 60.59%. Our study indicates that OsPAL2-1 is among the efficient genes that regulate rice allelopathy by controlling the synthesis of phenolic acid allelochemicals, and phenolic acid (ferulic acid, FA) induces the chemotactic aggregation of M. xanthus, which promoted the proliferation and aggregation of this microbe. The potential allelochemical, quercetin was generated from the FA-induced M. xanthus cultured medium. Frontiers Media S.A. 2020-07-23 /pmc/articles/PMC7391800/ /pubmed/32793125 http://dx.doi.org/10.3389/fmicb.2020.01411 Text en Copyright © 2020 Li, Jian, Li, Zeng, Xu, Khan and Lin. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Li, Yingzhe
Jian, Xin
Li, Yue
Zeng, Xiaomei
Xu, Lining
Khan, Muhammad Umar
Lin, Wenxiong
OsPAL2-1 Mediates Allelopathic Interactions Between Rice and Specific Microorganisms in the Rhizosphere Ecosystem
title OsPAL2-1 Mediates Allelopathic Interactions Between Rice and Specific Microorganisms in the Rhizosphere Ecosystem
title_full OsPAL2-1 Mediates Allelopathic Interactions Between Rice and Specific Microorganisms in the Rhizosphere Ecosystem
title_fullStr OsPAL2-1 Mediates Allelopathic Interactions Between Rice and Specific Microorganisms in the Rhizosphere Ecosystem
title_full_unstemmed OsPAL2-1 Mediates Allelopathic Interactions Between Rice and Specific Microorganisms in the Rhizosphere Ecosystem
title_short OsPAL2-1 Mediates Allelopathic Interactions Between Rice and Specific Microorganisms in the Rhizosphere Ecosystem
title_sort ospal2-1 mediates allelopathic interactions between rice and specific microorganisms in the rhizosphere ecosystem
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7391800/
https://www.ncbi.nlm.nih.gov/pubmed/32793125
http://dx.doi.org/10.3389/fmicb.2020.01411
work_keys_str_mv AT liyingzhe ospal21mediatesallelopathicinteractionsbetweenriceandspecificmicroorganismsintherhizosphereecosystem
AT jianxin ospal21mediatesallelopathicinteractionsbetweenriceandspecificmicroorganismsintherhizosphereecosystem
AT liyue ospal21mediatesallelopathicinteractionsbetweenriceandspecificmicroorganismsintherhizosphereecosystem
AT zengxiaomei ospal21mediatesallelopathicinteractionsbetweenriceandspecificmicroorganismsintherhizosphereecosystem
AT xulining ospal21mediatesallelopathicinteractionsbetweenriceandspecificmicroorganismsintherhizosphereecosystem
AT khanmuhammadumar ospal21mediatesallelopathicinteractionsbetweenriceandspecificmicroorganismsintherhizosphereecosystem
AT linwenxiong ospal21mediatesallelopathicinteractionsbetweenriceandspecificmicroorganismsintherhizosphereecosystem