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Parasite-derived circulating microRNAs as biomarkers for the detection of human Schistosoma japonicum infection
Novel tools for early diagnosis and monitoring of schistosomiasis are urgently needed. This study aimed to validate parasite-derived miRNAs as potential novel biomarkers for the detection of human Schistosoma japonicum infection. A total of 21 miRNAs were initially validated by real-time-polymerase...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cambridge University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7391863/ https://www.ncbi.nlm.nih.gov/pubmed/31840631 http://dx.doi.org/10.1017/S0031182019001690 |
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author | Mu, Yi Cai, Pengfei Olveda, Remigio M. Ross, Allen G. Olveda, David U. McManus, Donald P. |
author_facet | Mu, Yi Cai, Pengfei Olveda, Remigio M. Ross, Allen G. Olveda, David U. McManus, Donald P. |
author_sort | Mu, Yi |
collection | PubMed |
description | Novel tools for early diagnosis and monitoring of schistosomiasis are urgently needed. This study aimed to validate parasite-derived miRNAs as potential novel biomarkers for the detection of human Schistosoma japonicum infection. A total of 21 miRNAs were initially validated by real-time-polymerase chain reaction (RT-PCR) using serum samples of S. japonicum-infected BALB/c mice. Of these, 6 miRNAs were further validated with a human cohort of individuals from a schistosomiasis-endemic area of the Philippines. RT-PCR analysis showed that two parasite-derived miRNAs (sja-miR-2b-5p and sja-miR-2c-5p) could detect infected individuals with low infection intensity with moderate sensitivity/specificity values of 66%/68% and 55%/80%, respectively. Analysis of the combined data for the two parasite miRNAs revealed a specificity of 77.4% and a sensitivity of 60.0% with an area under the curve (AUC) value of 0.6906 (P = 0.0069); however, a duplex RT-PCR targeting both sja-miR-2b-5p and sja-miR-2c-5p did not result in an increased diagnostic performance compared with the singleplex assays. Furthermore, the serum level of sja-miR-2c-5p correlated significantly with faecal egg counts, whereas the other five miRNAs did not. Targeting S. japonicum-derived miRNAs in serum resulted in a moderate diagnostic performance when applied to a low schistosome infection intensity setting. |
format | Online Article Text |
id | pubmed-7391863 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Cambridge University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-73918632020-08-07 Parasite-derived circulating microRNAs as biomarkers for the detection of human Schistosoma japonicum infection Mu, Yi Cai, Pengfei Olveda, Remigio M. Ross, Allen G. Olveda, David U. McManus, Donald P. Parasitology Research Article Novel tools for early diagnosis and monitoring of schistosomiasis are urgently needed. This study aimed to validate parasite-derived miRNAs as potential novel biomarkers for the detection of human Schistosoma japonicum infection. A total of 21 miRNAs were initially validated by real-time-polymerase chain reaction (RT-PCR) using serum samples of S. japonicum-infected BALB/c mice. Of these, 6 miRNAs were further validated with a human cohort of individuals from a schistosomiasis-endemic area of the Philippines. RT-PCR analysis showed that two parasite-derived miRNAs (sja-miR-2b-5p and sja-miR-2c-5p) could detect infected individuals with low infection intensity with moderate sensitivity/specificity values of 66%/68% and 55%/80%, respectively. Analysis of the combined data for the two parasite miRNAs revealed a specificity of 77.4% and a sensitivity of 60.0% with an area under the curve (AUC) value of 0.6906 (P = 0.0069); however, a duplex RT-PCR targeting both sja-miR-2b-5p and sja-miR-2c-5p did not result in an increased diagnostic performance compared with the singleplex assays. Furthermore, the serum level of sja-miR-2c-5p correlated significantly with faecal egg counts, whereas the other five miRNAs did not. Targeting S. japonicum-derived miRNAs in serum resulted in a moderate diagnostic performance when applied to a low schistosome infection intensity setting. Cambridge University Press 2020-07 2019-12-16 /pmc/articles/PMC7391863/ /pubmed/31840631 http://dx.doi.org/10.1017/S0031182019001690 Text en © The Author(s) 2019 https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is unaltered and is properly cited. The written permission of Cambridge University Press must be obtained for commercial re-use or in order to create a derivative work. |
spellingShingle | Research Article Mu, Yi Cai, Pengfei Olveda, Remigio M. Ross, Allen G. Olveda, David U. McManus, Donald P. Parasite-derived circulating microRNAs as biomarkers for the detection of human Schistosoma japonicum infection |
title | Parasite-derived circulating microRNAs as biomarkers for the detection of human Schistosoma japonicum infection |
title_full | Parasite-derived circulating microRNAs as biomarkers for the detection of human Schistosoma japonicum infection |
title_fullStr | Parasite-derived circulating microRNAs as biomarkers for the detection of human Schistosoma japonicum infection |
title_full_unstemmed | Parasite-derived circulating microRNAs as biomarkers for the detection of human Schistosoma japonicum infection |
title_short | Parasite-derived circulating microRNAs as biomarkers for the detection of human Schistosoma japonicum infection |
title_sort | parasite-derived circulating micrornas as biomarkers for the detection of human schistosoma japonicum infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7391863/ https://www.ncbi.nlm.nih.gov/pubmed/31840631 http://dx.doi.org/10.1017/S0031182019001690 |
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