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Parasite-derived circulating microRNAs as biomarkers for the detection of human Schistosoma japonicum infection

Novel tools for early diagnosis and monitoring of schistosomiasis are urgently needed. This study aimed to validate parasite-derived miRNAs as potential novel biomarkers for the detection of human Schistosoma japonicum infection. A total of 21 miRNAs were initially validated by real-time-polymerase...

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Autores principales: Mu, Yi, Cai, Pengfei, Olveda, Remigio M., Ross, Allen G., Olveda, David U., McManus, Donald P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cambridge University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7391863/
https://www.ncbi.nlm.nih.gov/pubmed/31840631
http://dx.doi.org/10.1017/S0031182019001690
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author Mu, Yi
Cai, Pengfei
Olveda, Remigio M.
Ross, Allen G.
Olveda, David U.
McManus, Donald P.
author_facet Mu, Yi
Cai, Pengfei
Olveda, Remigio M.
Ross, Allen G.
Olveda, David U.
McManus, Donald P.
author_sort Mu, Yi
collection PubMed
description Novel tools for early diagnosis and monitoring of schistosomiasis are urgently needed. This study aimed to validate parasite-derived miRNAs as potential novel biomarkers for the detection of human Schistosoma japonicum infection. A total of 21 miRNAs were initially validated by real-time-polymerase chain reaction (RT-PCR) using serum samples of S. japonicum-infected BALB/c mice. Of these, 6 miRNAs were further validated with a human cohort of individuals from a schistosomiasis-endemic area of the Philippines. RT-PCR analysis showed that two parasite-derived miRNAs (sja-miR-2b-5p and sja-miR-2c-5p) could detect infected individuals with low infection intensity with moderate sensitivity/specificity values of 66%/68% and 55%/80%, respectively. Analysis of the combined data for the two parasite miRNAs revealed a specificity of 77.4% and a sensitivity of 60.0% with an area under the curve (AUC) value of 0.6906 (P = 0.0069); however, a duplex RT-PCR targeting both sja-miR-2b-5p and sja-miR-2c-5p did not result in an increased diagnostic performance compared with the singleplex assays. Furthermore, the serum level of sja-miR-2c-5p correlated significantly with faecal egg counts, whereas the other five miRNAs did not. Targeting S. japonicum-derived miRNAs in serum resulted in a moderate diagnostic performance when applied to a low schistosome infection intensity setting.
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spelling pubmed-73918632020-08-07 Parasite-derived circulating microRNAs as biomarkers for the detection of human Schistosoma japonicum infection Mu, Yi Cai, Pengfei Olveda, Remigio M. Ross, Allen G. Olveda, David U. McManus, Donald P. Parasitology Research Article Novel tools for early diagnosis and monitoring of schistosomiasis are urgently needed. This study aimed to validate parasite-derived miRNAs as potential novel biomarkers for the detection of human Schistosoma japonicum infection. A total of 21 miRNAs were initially validated by real-time-polymerase chain reaction (RT-PCR) using serum samples of S. japonicum-infected BALB/c mice. Of these, 6 miRNAs were further validated with a human cohort of individuals from a schistosomiasis-endemic area of the Philippines. RT-PCR analysis showed that two parasite-derived miRNAs (sja-miR-2b-5p and sja-miR-2c-5p) could detect infected individuals with low infection intensity with moderate sensitivity/specificity values of 66%/68% and 55%/80%, respectively. Analysis of the combined data for the two parasite miRNAs revealed a specificity of 77.4% and a sensitivity of 60.0% with an area under the curve (AUC) value of 0.6906 (P = 0.0069); however, a duplex RT-PCR targeting both sja-miR-2b-5p and sja-miR-2c-5p did not result in an increased diagnostic performance compared with the singleplex assays. Furthermore, the serum level of sja-miR-2c-5p correlated significantly with faecal egg counts, whereas the other five miRNAs did not. Targeting S. japonicum-derived miRNAs in serum resulted in a moderate diagnostic performance when applied to a low schistosome infection intensity setting. Cambridge University Press 2020-07 2019-12-16 /pmc/articles/PMC7391863/ /pubmed/31840631 http://dx.doi.org/10.1017/S0031182019001690 Text en © The Author(s) 2019 https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is unaltered and is properly cited. The written permission of Cambridge University Press must be obtained for commercial re-use or in order to create a derivative work.
spellingShingle Research Article
Mu, Yi
Cai, Pengfei
Olveda, Remigio M.
Ross, Allen G.
Olveda, David U.
McManus, Donald P.
Parasite-derived circulating microRNAs as biomarkers for the detection of human Schistosoma japonicum infection
title Parasite-derived circulating microRNAs as biomarkers for the detection of human Schistosoma japonicum infection
title_full Parasite-derived circulating microRNAs as biomarkers for the detection of human Schistosoma japonicum infection
title_fullStr Parasite-derived circulating microRNAs as biomarkers for the detection of human Schistosoma japonicum infection
title_full_unstemmed Parasite-derived circulating microRNAs as biomarkers for the detection of human Schistosoma japonicum infection
title_short Parasite-derived circulating microRNAs as biomarkers for the detection of human Schistosoma japonicum infection
title_sort parasite-derived circulating micrornas as biomarkers for the detection of human schistosoma japonicum infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7391863/
https://www.ncbi.nlm.nih.gov/pubmed/31840631
http://dx.doi.org/10.1017/S0031182019001690
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