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Effects of Yacon on Colonic IFN-γ and Goblet Cells of the 2,4,6-Trinitrobenzene Sulfonic Acid-Induced Colitis Mouse Model

BACKGROUND: IBD is a chronic inflammatory condition associated with damage to the intestinal mucosal barrier. Supplementation of yacon tubers has been known to have positive effect on intestinal health. Therefore, we conducted the present study to investigate the effect of yacon tuber powder on Th1...

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Autores principales: Umizah, Larasti Putri, Wasityastuti, Widya, Widasari, Dewiyani Indah, Purwono, Setyo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pasteur Institute of Iran 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7392140/
https://www.ncbi.nlm.nih.gov/pubmed/32429633
http://dx.doi.org/10.29252/ibj.24.5.276
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author Umizah, Larasti Putri
Wasityastuti, Widya
Widasari, Dewiyani Indah
Purwono, Setyo
author_facet Umizah, Larasti Putri
Wasityastuti, Widya
Widasari, Dewiyani Indah
Purwono, Setyo
author_sort Umizah, Larasti Putri
collection PubMed
description BACKGROUND: IBD is a chronic inflammatory condition associated with damage to the intestinal mucosal barrier. Supplementation of yacon tubers has been known to have positive effect on intestinal health. Therefore, we conducted the present study to investigate the effect of yacon tuber powder on Th1 activation pathway by evaluating IFN-γ levels and the number of goblet cells in the colon of colitis mouse models. METHODS: Thirty BALB/c mice were divided into five groups: 1, control group (G1) and 2-5, TNBS-induced colitis groups (GII-GV). Yacon powder was given to three of the TNBS-induced colitis groups with the doses of 0.165 g/30 g BW (GIII), 0.331 g/30 g BW (GIV), and 0.662 g/30 g BW (GV) for 14 days. IFN-γ levels were assessed using ELISA, while number of goblet cells was calculated based on histological observation. RESULTS: Significantly lower IFN-γ levels was observed in GV compared to colitis group (GII) (p = 0.007). GV also showed significantly higher number of goblet cells per 100 epithelial cells in 20 crypts (p = 0.000) than GII. CONCLUSION: The administration of yacon powder at a dose of 0.662 g/30 g BW could decrease IFN-γ levels and improve the healing of intestinal mucosa in colitis mouse models by increasing the number of goblet cells.
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spelling pubmed-73921402020-09-01 Effects of Yacon on Colonic IFN-γ and Goblet Cells of the 2,4,6-Trinitrobenzene Sulfonic Acid-Induced Colitis Mouse Model Umizah, Larasti Putri Wasityastuti, Widya Widasari, Dewiyani Indah Purwono, Setyo Iran Biomed J Full Length BACKGROUND: IBD is a chronic inflammatory condition associated with damage to the intestinal mucosal barrier. Supplementation of yacon tubers has been known to have positive effect on intestinal health. Therefore, we conducted the present study to investigate the effect of yacon tuber powder on Th1 activation pathway by evaluating IFN-γ levels and the number of goblet cells in the colon of colitis mouse models. METHODS: Thirty BALB/c mice were divided into five groups: 1, control group (G1) and 2-5, TNBS-induced colitis groups (GII-GV). Yacon powder was given to three of the TNBS-induced colitis groups with the doses of 0.165 g/30 g BW (GIII), 0.331 g/30 g BW (GIV), and 0.662 g/30 g BW (GV) for 14 days. IFN-γ levels were assessed using ELISA, while number of goblet cells was calculated based on histological observation. RESULTS: Significantly lower IFN-γ levels was observed in GV compared to colitis group (GII) (p = 0.007). GV also showed significantly higher number of goblet cells per 100 epithelial cells in 20 crypts (p = 0.000) than GII. CONCLUSION: The administration of yacon powder at a dose of 0.662 g/30 g BW could decrease IFN-γ levels and improve the healing of intestinal mucosa in colitis mouse models by increasing the number of goblet cells. Pasteur Institute of Iran 2020-09 2020-01-20 /pmc/articles/PMC7392140/ /pubmed/32429633 http://dx.doi.org/10.29252/ibj.24.5.276 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Full Length
Umizah, Larasti Putri
Wasityastuti, Widya
Widasari, Dewiyani Indah
Purwono, Setyo
Effects of Yacon on Colonic IFN-γ and Goblet Cells of the 2,4,6-Trinitrobenzene Sulfonic Acid-Induced Colitis Mouse Model
title Effects of Yacon on Colonic IFN-γ and Goblet Cells of the 2,4,6-Trinitrobenzene Sulfonic Acid-Induced Colitis Mouse Model
title_full Effects of Yacon on Colonic IFN-γ and Goblet Cells of the 2,4,6-Trinitrobenzene Sulfonic Acid-Induced Colitis Mouse Model
title_fullStr Effects of Yacon on Colonic IFN-γ and Goblet Cells of the 2,4,6-Trinitrobenzene Sulfonic Acid-Induced Colitis Mouse Model
title_full_unstemmed Effects of Yacon on Colonic IFN-γ and Goblet Cells of the 2,4,6-Trinitrobenzene Sulfonic Acid-Induced Colitis Mouse Model
title_short Effects of Yacon on Colonic IFN-γ and Goblet Cells of the 2,4,6-Trinitrobenzene Sulfonic Acid-Induced Colitis Mouse Model
title_sort effects of yacon on colonic ifn-γ and goblet cells of the 2,4,6-trinitrobenzene sulfonic acid-induced colitis mouse model
topic Full Length
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7392140/
https://www.ncbi.nlm.nih.gov/pubmed/32429633
http://dx.doi.org/10.29252/ibj.24.5.276
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