Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells in Fully Chemically Defined Conditions

In the past few years, several different methods for differentiation of directed cardiomyocytes from human pluripotent stem cells (hPSCs) in chemically defined conditions have been reported, including our own (Burridge et al., 2014, Lian et al., 2012, Lin et al., 2017). To help researchers adapt to...

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Detalles Bibliográficos
Autores principales: Lin, Yongshun, Zou, Jizhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7392178/
https://www.ncbi.nlm.nih.gov/pubmed/32734277
http://dx.doi.org/10.1016/j.xpro.2020.100015
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author Lin, Yongshun
Zou, Jizhong
author_facet Lin, Yongshun
Zou, Jizhong
author_sort Lin, Yongshun
collection PubMed
description In the past few years, several different methods for differentiation of directed cardiomyocytes from human pluripotent stem cells (hPSCs) in chemically defined conditions have been reported, including our own (Burridge et al., 2014, Lian et al., 2012, Lin et al., 2017). To help researchers adapt to our simple and cost-effective method, we provide step-by-step protocols for hPSC-cardiomyocyte differentiation, including hPSC culture, cardiomyocyte differentiation, cardiomyocyte passaging, and cryopreservation. For complete details on the use and execution of this protocol, please refer to Lin et al. (2017).
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spelling pubmed-73921782020-07-30 Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells in Fully Chemically Defined Conditions Lin, Yongshun Zou, Jizhong STAR Protoc Protocol In the past few years, several different methods for differentiation of directed cardiomyocytes from human pluripotent stem cells (hPSCs) in chemically defined conditions have been reported, including our own (Burridge et al., 2014, Lian et al., 2012, Lin et al., 2017). To help researchers adapt to our simple and cost-effective method, we provide step-by-step protocols for hPSC-cardiomyocyte differentiation, including hPSC culture, cardiomyocyte differentiation, cardiomyocyte passaging, and cryopreservation. For complete details on the use and execution of this protocol, please refer to Lin et al. (2017). Elsevier 2020-06-03 /pmc/articles/PMC7392178/ /pubmed/32734277 http://dx.doi.org/10.1016/j.xpro.2020.100015 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Lin, Yongshun
Zou, Jizhong
Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells in Fully Chemically Defined Conditions
title Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells in Fully Chemically Defined Conditions
title_full Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells in Fully Chemically Defined Conditions
title_fullStr Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells in Fully Chemically Defined Conditions
title_full_unstemmed Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells in Fully Chemically Defined Conditions
title_short Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells in Fully Chemically Defined Conditions
title_sort differentiation of cardiomyocytes from human pluripotent stem cells in fully chemically defined conditions
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7392178/
https://www.ncbi.nlm.nih.gov/pubmed/32734277
http://dx.doi.org/10.1016/j.xpro.2020.100015
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AT zoujizhong differentiationofcardiomyocytesfromhumanpluripotentstemcellsinfullychemicallydefinedconditions

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