Protection of human intestinal epithelial cells from oxidative stress caused by mercury using lactic acid bacteria

Heavy metals are harmful to human health. Therefore, we investigated the biosorption of heavy metals by lactic acid bacteria (LAB). Of all the tested heavy metals, biosorption by LAB was highest for mercury, followed by lead, cadmium, and finally arsenic. The viability of HCT-116 cells was reduced b...

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Autores principales: KINOSHITA, Hideki, JUMONJI, Mai, YASUDA, Shin, IGOSHI, Keiji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMFH Press 2020
Materias:
Full Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7392913/
https://www.ncbi.nlm.nih.gov/pubmed/32775138
http://dx.doi.org/10.12938/bmfh.2019-049
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author KINOSHITA, Hideki
JUMONJI, Mai
YASUDA, Shin
IGOSHI, Keiji
author_facet KINOSHITA, Hideki
JUMONJI, Mai
YASUDA, Shin
IGOSHI, Keiji
author_sort KINOSHITA, Hideki
collection PubMed
description Heavy metals are harmful to human health. Therefore, we investigated the biosorption of heavy metals by lactic acid bacteria (LAB). Of all the tested heavy metals, biosorption by LAB was highest for mercury, followed by lead, cadmium, and finally arsenic. The viability of HCT-116 cells was reduced by half in the presence of 7.5 µg/mL mercury but recovered after the addition of selected LAB strains. HCT-116 cells showed increased superoxide dismutase and catalase activities, whereas glutathione peroxidase activities decreased significantly. Addition of Lactobacillus sakei TOKAI 57m recovered all antioxidant enzyme activities. Our results suggest that this strain can be used for cellular detoxification.
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spelling pubmed-73929132020-08-07 Protection of human intestinal epithelial cells from oxidative stress caused by mercury using lactic acid bacteria KINOSHITA, Hideki JUMONJI, Mai YASUDA, Shin IGOSHI, Keiji Biosci Microbiota Food Health Full Paper Heavy metals are harmful to human health. Therefore, we investigated the biosorption of heavy metals by lactic acid bacteria (LAB). Of all the tested heavy metals, biosorption by LAB was highest for mercury, followed by lead, cadmium, and finally arsenic. The viability of HCT-116 cells was reduced by half in the presence of 7.5 µg/mL mercury but recovered after the addition of selected LAB strains. HCT-116 cells showed increased superoxide dismutase and catalase activities, whereas glutathione peroxidase activities decreased significantly. Addition of Lactobacillus sakei TOKAI 57m recovered all antioxidant enzyme activities. Our results suggest that this strain can be used for cellular detoxification. BMFH Press 2020-05-02 2020 /pmc/articles/PMC7392913/ /pubmed/32775138 http://dx.doi.org/10.12938/bmfh.2019-049 Text en ©2020 BMFH Press This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Full Paper
KINOSHITA, Hideki
JUMONJI, Mai
YASUDA, Shin
IGOSHI, Keiji
Protection of human intestinal epithelial cells from oxidative stress caused by mercury using lactic acid bacteria
title Protection of human intestinal epithelial cells from oxidative stress caused by mercury using lactic acid bacteria
title_full Protection of human intestinal epithelial cells from oxidative stress caused by mercury using lactic acid bacteria
title_fullStr Protection of human intestinal epithelial cells from oxidative stress caused by mercury using lactic acid bacteria
title_full_unstemmed Protection of human intestinal epithelial cells from oxidative stress caused by mercury using lactic acid bacteria
title_short Protection of human intestinal epithelial cells from oxidative stress caused by mercury using lactic acid bacteria
title_sort protection of human intestinal epithelial cells from oxidative stress caused by mercury using lactic acid bacteria
topic Full Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7392913/
https://www.ncbi.nlm.nih.gov/pubmed/32775138
http://dx.doi.org/10.12938/bmfh.2019-049
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