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Protection of gut microbiome from antibiotics: development of a vancomycin-specific adsorbent with high adsorption capacity

The fraction of administered antibiotics that reach the cecum and colon causes dysbiosis of the gut microbiome, resulting in various diseases. Protection of the gut microbiome from antibiotics using antibiotic adsorbents in the cecum and colon is a promising method to overcome this issue. Previously...

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Autores principales: YUZURIHA, Kazuki, YAKABE, Kyosuke, NAGAI, Haruka, LI, Shunyi, ZENDO, Takeshi, ZAI, Khadijah, KISHIMURA, Akihiro, HASE, Koji, KIM, Yun-Gi, MORI, Takeshi, KATAYAMA, Yoshiki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMFH Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7392918/
https://www.ncbi.nlm.nih.gov/pubmed/32775131
http://dx.doi.org/10.12938/bmfh.2020-002
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author YUZURIHA, Kazuki
YAKABE, Kyosuke
NAGAI, Haruka
LI, Shunyi
ZENDO, Takeshi
ZAI, Khadijah
KISHIMURA, Akihiro
HASE, Koji
KIM, Yun-Gi
MORI, Takeshi
KATAYAMA, Yoshiki
author_facet YUZURIHA, Kazuki
YAKABE, Kyosuke
NAGAI, Haruka
LI, Shunyi
ZENDO, Takeshi
ZAI, Khadijah
KISHIMURA, Akihiro
HASE, Koji
KIM, Yun-Gi
MORI, Takeshi
KATAYAMA, Yoshiki
author_sort YUZURIHA, Kazuki
collection PubMed
description The fraction of administered antibiotics that reach the cecum and colon causes dysbiosis of the gut microbiome, resulting in various diseases. Protection of the gut microbiome from antibiotics using antibiotic adsorbents in the cecum and colon is a promising method to overcome this issue. Previously, activated charcoal (AC) has been reported to protect the gut microbiome of host animals. AC is an adsorbent that is widely used to capture toxic compounds and overdosed drugs in the gastrointestinal tract. The specificity of adsorbents for antibiotics is critical to avoid the risk of unexpected side effects caused by nonspecific adsorption of biological compounds in the intestinal fluid, such as bile acids and essential micronutrients. Here, we have developed specific adsorbents for vancomycin (VCM), which is known to cause gut dysbiosis. The adsorbents were composed of polyethyleneglycol-based microparticles (MPs) in which a specific ligand for VCM, D-Ala-D-Ala-OH, was attached via dendrons of D-lysine to raise the content of the ligand in the MPs. The MPs successfully protected Staphylococcus lentus from VCM in vitro because of the adsorption of VCM in the culture media. Pre-administration of MPs to mice reduced the amount of free VCM in the feces to an undetectable level. This treatment minimized the effect of VCM on gut microbiota and provided protection against Clostridioides difficile infection after oral challenge with spores.
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spelling pubmed-73929182020-08-07 Protection of gut microbiome from antibiotics: development of a vancomycin-specific adsorbent with high adsorption capacity YUZURIHA, Kazuki YAKABE, Kyosuke NAGAI, Haruka LI, Shunyi ZENDO, Takeshi ZAI, Khadijah KISHIMURA, Akihiro HASE, Koji KIM, Yun-Gi MORI, Takeshi KATAYAMA, Yoshiki Biosci Microbiota Food Health Full Paper The fraction of administered antibiotics that reach the cecum and colon causes dysbiosis of the gut microbiome, resulting in various diseases. Protection of the gut microbiome from antibiotics using antibiotic adsorbents in the cecum and colon is a promising method to overcome this issue. Previously, activated charcoal (AC) has been reported to protect the gut microbiome of host animals. AC is an adsorbent that is widely used to capture toxic compounds and overdosed drugs in the gastrointestinal tract. The specificity of adsorbents for antibiotics is critical to avoid the risk of unexpected side effects caused by nonspecific adsorption of biological compounds in the intestinal fluid, such as bile acids and essential micronutrients. Here, we have developed specific adsorbents for vancomycin (VCM), which is known to cause gut dysbiosis. The adsorbents were composed of polyethyleneglycol-based microparticles (MPs) in which a specific ligand for VCM, D-Ala-D-Ala-OH, was attached via dendrons of D-lysine to raise the content of the ligand in the MPs. The MPs successfully protected Staphylococcus lentus from VCM in vitro because of the adsorption of VCM in the culture media. Pre-administration of MPs to mice reduced the amount of free VCM in the feces to an undetectable level. This treatment minimized the effect of VCM on gut microbiota and provided protection against Clostridioides difficile infection after oral challenge with spores. BMFH Press 2020-02-29 2020 /pmc/articles/PMC7392918/ /pubmed/32775131 http://dx.doi.org/10.12938/bmfh.2020-002 Text en ©2020 BMFH Press This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Full Paper
YUZURIHA, Kazuki
YAKABE, Kyosuke
NAGAI, Haruka
LI, Shunyi
ZENDO, Takeshi
ZAI, Khadijah
KISHIMURA, Akihiro
HASE, Koji
KIM, Yun-Gi
MORI, Takeshi
KATAYAMA, Yoshiki
Protection of gut microbiome from antibiotics: development of a vancomycin-specific adsorbent with high adsorption capacity
title Protection of gut microbiome from antibiotics: development of a vancomycin-specific adsorbent with high adsorption capacity
title_full Protection of gut microbiome from antibiotics: development of a vancomycin-specific adsorbent with high adsorption capacity
title_fullStr Protection of gut microbiome from antibiotics: development of a vancomycin-specific adsorbent with high adsorption capacity
title_full_unstemmed Protection of gut microbiome from antibiotics: development of a vancomycin-specific adsorbent with high adsorption capacity
title_short Protection of gut microbiome from antibiotics: development of a vancomycin-specific adsorbent with high adsorption capacity
title_sort protection of gut microbiome from antibiotics: development of a vancomycin-specific adsorbent with high adsorption capacity
topic Full Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7392918/
https://www.ncbi.nlm.nih.gov/pubmed/32775131
http://dx.doi.org/10.12938/bmfh.2020-002
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