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Development of rapid colorimetric assay for the detection of Influenza A and B viruses

The flu viruses are respiratory pathogens which, according to the World Health Organization (WHO), infect 5–10% of the world population resulting in 3–5 million cases of severe illness and 290,000 to 650,000 annual deaths. Early diagnosis and therapeutic intervention can ameliorate symptoms of infec...

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Autores principales: Raji, Muhabat Adeola, Aloraij, Yumna, Alhamlan, Fatimah, Suaifan, Ghadeer, Weber, Karina, Cialla-May, Dana, Popp, Jürgen, Zourob, Mohammed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7392922/
https://www.ncbi.nlm.nih.gov/pubmed/33076087
http://dx.doi.org/10.1016/j.talanta.2020.121468
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author Raji, Muhabat Adeola
Aloraij, Yumna
Alhamlan, Fatimah
Suaifan, Ghadeer
Weber, Karina
Cialla-May, Dana
Popp, Jürgen
Zourob, Mohammed
author_facet Raji, Muhabat Adeola
Aloraij, Yumna
Alhamlan, Fatimah
Suaifan, Ghadeer
Weber, Karina
Cialla-May, Dana
Popp, Jürgen
Zourob, Mohammed
author_sort Raji, Muhabat Adeola
collection PubMed
description The flu viruses are respiratory pathogens which, according to the World Health Organization (WHO), infect 5–10% of the world population resulting in 3–5 million cases of severe illness and 290,000 to 650,000 annual deaths. Early diagnosis and therapeutic intervention can ameliorate symptoms of infection and reduce mortality. The conventional diagnosis of viral infections, including flu viruses, has evolved over the years with diverse approaches, however, there are inherent short comings associated with these testing. There is an urgent need for rapid and low-cost diagnostic assays, due to the enormous annual burden of influenza diseases and its associated mortality. In this study, novel, low cost and easy to use colorimetric flu virus biosensor assay was developed. The sandwich assay format was utilized using antibodies immobilized onto cotton swabs, for the rapid detection of flu A and B viruses. These swabs serve as sample collection, analytes pre-concentration as well as sensing tool. The proof of concept was established for this assay in buffer and mucus samples. The limit of detection (LOD) of the colorimetric assay was 0.04 ng mL(−1) for Flu A and Flu B respectively and with linear dynamic range between 0.04 ng ml(−1) to 40 ng ml for both viruses in mucous samples. The assay can be performed at the patient's bed side by minimally skilled hospital personnel without the need for instrumentation. Cross-reactivity assays testing was done using Flu viruses specific activated swabs reacted with other common respiratory viral pathogens' antigen, in order to assess the specificity of the swabs.
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spelling pubmed-73929222020-07-31 Development of rapid colorimetric assay for the detection of Influenza A and B viruses Raji, Muhabat Adeola Aloraij, Yumna Alhamlan, Fatimah Suaifan, Ghadeer Weber, Karina Cialla-May, Dana Popp, Jürgen Zourob, Mohammed Talanta Article The flu viruses are respiratory pathogens which, according to the World Health Organization (WHO), infect 5–10% of the world population resulting in 3–5 million cases of severe illness and 290,000 to 650,000 annual deaths. Early diagnosis and therapeutic intervention can ameliorate symptoms of infection and reduce mortality. The conventional diagnosis of viral infections, including flu viruses, has evolved over the years with diverse approaches, however, there are inherent short comings associated with these testing. There is an urgent need for rapid and low-cost diagnostic assays, due to the enormous annual burden of influenza diseases and its associated mortality. In this study, novel, low cost and easy to use colorimetric flu virus biosensor assay was developed. The sandwich assay format was utilized using antibodies immobilized onto cotton swabs, for the rapid detection of flu A and B viruses. These swabs serve as sample collection, analytes pre-concentration as well as sensing tool. The proof of concept was established for this assay in buffer and mucus samples. The limit of detection (LOD) of the colorimetric assay was 0.04 ng mL(−1) for Flu A and Flu B respectively and with linear dynamic range between 0.04 ng ml(−1) to 40 ng ml for both viruses in mucous samples. The assay can be performed at the patient's bed side by minimally skilled hospital personnel without the need for instrumentation. Cross-reactivity assays testing was done using Flu viruses specific activated swabs reacted with other common respiratory viral pathogens' antigen, in order to assess the specificity of the swabs. Elsevier B.V. 2021-01-01 2020-07-31 /pmc/articles/PMC7392922/ /pubmed/33076087 http://dx.doi.org/10.1016/j.talanta.2020.121468 Text en © 2020 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Raji, Muhabat Adeola
Aloraij, Yumna
Alhamlan, Fatimah
Suaifan, Ghadeer
Weber, Karina
Cialla-May, Dana
Popp, Jürgen
Zourob, Mohammed
Development of rapid colorimetric assay for the detection of Influenza A and B viruses
title Development of rapid colorimetric assay for the detection of Influenza A and B viruses
title_full Development of rapid colorimetric assay for the detection of Influenza A and B viruses
title_fullStr Development of rapid colorimetric assay for the detection of Influenza A and B viruses
title_full_unstemmed Development of rapid colorimetric assay for the detection of Influenza A and B viruses
title_short Development of rapid colorimetric assay for the detection of Influenza A and B viruses
title_sort development of rapid colorimetric assay for the detection of influenza a and b viruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7392922/
https://www.ncbi.nlm.nih.gov/pubmed/33076087
http://dx.doi.org/10.1016/j.talanta.2020.121468
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