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Plant cell (Brassica napus) response to europium(III) and uranium(VI) exposure

Experiments conducted over a period of 6 weeks using Brassica napus callus cells grown in vitro under Eu(III) or U(VI) stress showed that B. napus cells were able to bioassociate both potentially toxic metals (PTM), 628 nmol Eu/g(fresh cells) and 995 nmol U/g(fresh cells). Most of the Eu(III) and U(...

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Autores principales: Moll, Henry, Sachs, Susanne, Geipel, Gerhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7392935/
https://www.ncbi.nlm.nih.gov/pubmed/32504441
http://dx.doi.org/10.1007/s11356-020-09525-2
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author Moll, Henry
Sachs, Susanne
Geipel, Gerhard
author_facet Moll, Henry
Sachs, Susanne
Geipel, Gerhard
author_sort Moll, Henry
collection PubMed
description Experiments conducted over a period of 6 weeks using Brassica napus callus cells grown in vitro under Eu(III) or U(VI) stress showed that B. napus cells were able to bioassociate both potentially toxic metals (PTM), 628 nmol Eu/g(fresh cells) and 995 nmol U/g(fresh cells). Most of the Eu(III) and U(VI) was found to be enriched in the cell wall fraction. Under high metal stress (200 μM), cells responded with reduced cell viability and growth. Subsequent speciation analyses using both metals as luminescence probes confirmed that B. napus callus cells provided multiple-binding environments for Eu(III) and U(VI). Moreover, two different inner-sphere Eu(3+) species could be distinguished. For U(VI), a dominant binding by organic and/or inorganic phosphate groups of the plant biomass can be concluded. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11356-020-09525-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-73929352020-08-12 Plant cell (Brassica napus) response to europium(III) and uranium(VI) exposure Moll, Henry Sachs, Susanne Geipel, Gerhard Environ Sci Pollut Res Int Research Article Experiments conducted over a period of 6 weeks using Brassica napus callus cells grown in vitro under Eu(III) or U(VI) stress showed that B. napus cells were able to bioassociate both potentially toxic metals (PTM), 628 nmol Eu/g(fresh cells) and 995 nmol U/g(fresh cells). Most of the Eu(III) and U(VI) was found to be enriched in the cell wall fraction. Under high metal stress (200 μM), cells responded with reduced cell viability and growth. Subsequent speciation analyses using both metals as luminescence probes confirmed that B. napus callus cells provided multiple-binding environments for Eu(III) and U(VI). Moreover, two different inner-sphere Eu(3+) species could be distinguished. For U(VI), a dominant binding by organic and/or inorganic phosphate groups of the plant biomass can be concluded. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11356-020-09525-2) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2020-06-06 2020 /pmc/articles/PMC7392935/ /pubmed/32504441 http://dx.doi.org/10.1007/s11356-020-09525-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Moll, Henry
Sachs, Susanne
Geipel, Gerhard
Plant cell (Brassica napus) response to europium(III) and uranium(VI) exposure
title Plant cell (Brassica napus) response to europium(III) and uranium(VI) exposure
title_full Plant cell (Brassica napus) response to europium(III) and uranium(VI) exposure
title_fullStr Plant cell (Brassica napus) response to europium(III) and uranium(VI) exposure
title_full_unstemmed Plant cell (Brassica napus) response to europium(III) and uranium(VI) exposure
title_short Plant cell (Brassica napus) response to europium(III) and uranium(VI) exposure
title_sort plant cell (brassica napus) response to europium(iii) and uranium(vi) exposure
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7392935/
https://www.ncbi.nlm.nih.gov/pubmed/32504441
http://dx.doi.org/10.1007/s11356-020-09525-2
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