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Plant cell (Brassica napus) response to europium(III) and uranium(VI) exposure
Experiments conducted over a period of 6 weeks using Brassica napus callus cells grown in vitro under Eu(III) or U(VI) stress showed that B. napus cells were able to bioassociate both potentially toxic metals (PTM), 628 nmol Eu/g(fresh cells) and 995 nmol U/g(fresh cells). Most of the Eu(III) and U(...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7392935/ https://www.ncbi.nlm.nih.gov/pubmed/32504441 http://dx.doi.org/10.1007/s11356-020-09525-2 |
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author | Moll, Henry Sachs, Susanne Geipel, Gerhard |
author_facet | Moll, Henry Sachs, Susanne Geipel, Gerhard |
author_sort | Moll, Henry |
collection | PubMed |
description | Experiments conducted over a period of 6 weeks using Brassica napus callus cells grown in vitro under Eu(III) or U(VI) stress showed that B. napus cells were able to bioassociate both potentially toxic metals (PTM), 628 nmol Eu/g(fresh cells) and 995 nmol U/g(fresh cells). Most of the Eu(III) and U(VI) was found to be enriched in the cell wall fraction. Under high metal stress (200 μM), cells responded with reduced cell viability and growth. Subsequent speciation analyses using both metals as luminescence probes confirmed that B. napus callus cells provided multiple-binding environments for Eu(III) and U(VI). Moreover, two different inner-sphere Eu(3+) species could be distinguished. For U(VI), a dominant binding by organic and/or inorganic phosphate groups of the plant biomass can be concluded. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11356-020-09525-2) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-7392935 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-73929352020-08-12 Plant cell (Brassica napus) response to europium(III) and uranium(VI) exposure Moll, Henry Sachs, Susanne Geipel, Gerhard Environ Sci Pollut Res Int Research Article Experiments conducted over a period of 6 weeks using Brassica napus callus cells grown in vitro under Eu(III) or U(VI) stress showed that B. napus cells were able to bioassociate both potentially toxic metals (PTM), 628 nmol Eu/g(fresh cells) and 995 nmol U/g(fresh cells). Most of the Eu(III) and U(VI) was found to be enriched in the cell wall fraction. Under high metal stress (200 μM), cells responded with reduced cell viability and growth. Subsequent speciation analyses using both metals as luminescence probes confirmed that B. napus callus cells provided multiple-binding environments for Eu(III) and U(VI). Moreover, two different inner-sphere Eu(3+) species could be distinguished. For U(VI), a dominant binding by organic and/or inorganic phosphate groups of the plant biomass can be concluded. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11356-020-09525-2) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2020-06-06 2020 /pmc/articles/PMC7392935/ /pubmed/32504441 http://dx.doi.org/10.1007/s11356-020-09525-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Moll, Henry Sachs, Susanne Geipel, Gerhard Plant cell (Brassica napus) response to europium(III) and uranium(VI) exposure |
title | Plant cell (Brassica napus) response to europium(III) and uranium(VI) exposure |
title_full | Plant cell (Brassica napus) response to europium(III) and uranium(VI) exposure |
title_fullStr | Plant cell (Brassica napus) response to europium(III) and uranium(VI) exposure |
title_full_unstemmed | Plant cell (Brassica napus) response to europium(III) and uranium(VI) exposure |
title_short | Plant cell (Brassica napus) response to europium(III) and uranium(VI) exposure |
title_sort | plant cell (brassica napus) response to europium(iii) and uranium(vi) exposure |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7392935/ https://www.ncbi.nlm.nih.gov/pubmed/32504441 http://dx.doi.org/10.1007/s11356-020-09525-2 |
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