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Synergic Effects of Temperature and Irradiance on the Physiology of the Marine Synechococcus Strain WH7803

Understanding how microorganisms adjust their metabolism to maintain their ability to cope with short-term environmental variations constitutes one of the major current challenges in microbial ecology. Here, the best physiologically characterized marine Synechococcus strain, WH7803, was exposed to m...

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Autores principales: Guyet, Ulysse, Nguyen, Ngoc A., Doré, Hugo, Haguait, Julie, Pittera, Justine, Conan, Maël, Ratin, Morgane, Corre, Erwan, Le Corguillé, Gildas, Brillet-Guéguen, Loraine, Hoebeke, Mark, Six, Christophe, Steglich, Claudia, Siegel, Anne, Eveillard, Damien, Partensky, Frédéric, Garczarek, Laurence
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7393227/
https://www.ncbi.nlm.nih.gov/pubmed/32793165
http://dx.doi.org/10.3389/fmicb.2020.01707
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author Guyet, Ulysse
Nguyen, Ngoc A.
Doré, Hugo
Haguait, Julie
Pittera, Justine
Conan, Maël
Ratin, Morgane
Corre, Erwan
Le Corguillé, Gildas
Brillet-Guéguen, Loraine
Hoebeke, Mark
Six, Christophe
Steglich, Claudia
Siegel, Anne
Eveillard, Damien
Partensky, Frédéric
Garczarek, Laurence
author_facet Guyet, Ulysse
Nguyen, Ngoc A.
Doré, Hugo
Haguait, Julie
Pittera, Justine
Conan, Maël
Ratin, Morgane
Corre, Erwan
Le Corguillé, Gildas
Brillet-Guéguen, Loraine
Hoebeke, Mark
Six, Christophe
Steglich, Claudia
Siegel, Anne
Eveillard, Damien
Partensky, Frédéric
Garczarek, Laurence
author_sort Guyet, Ulysse
collection PubMed
description Understanding how microorganisms adjust their metabolism to maintain their ability to cope with short-term environmental variations constitutes one of the major current challenges in microbial ecology. Here, the best physiologically characterized marine Synechococcus strain, WH7803, was exposed to modulated light/dark cycles or acclimated to continuous high-light (HL) or low-light (LL), then shifted to various stress conditions, including low (LT) or high temperature (HT), HL and ultraviolet (UV) radiations. Physiological responses were analyzed by measuring time courses of photosystem (PS) II quantum yield, PSII repair rate, pigment ratios and global changes in gene expression. Previously published membrane lipid composition were also used for correlation analyses. These data revealed that cells previously acclimated to HL are better prepared than LL-acclimated cells to sustain an additional light or UV stress, but not a LT stress. Indeed, LT seems to induce a synergic effect with the HL treatment, as previously observed with oxidative stress. While all tested shift conditions induced the downregulation of many photosynthetic genes, notably those encoding PSI, cytochrome b(6)/f and phycobilisomes, UV stress proved to be more deleterious for PSII than the other treatments, and full recovery of damaged PSII from UV stress seemed to involve the neo-synthesis of a fairly large number of PSII subunits and not just the reassembly of pre-existing subunits after D1 replacement. In contrast, genes involved in glycogen degradation and carotenoid biosynthesis pathways were more particularly upregulated in response to LT. Altogether, these experiments allowed us to identify responses common to all stresses and those more specific to a given stress, thus highlighting genes potentially involved in niche acclimation of a key member of marine ecosystems. Our data also revealed important specific features of the stress responses compared to model freshwater cyanobacteria.
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spelling pubmed-73932272020-08-12 Synergic Effects of Temperature and Irradiance on the Physiology of the Marine Synechococcus Strain WH7803 Guyet, Ulysse Nguyen, Ngoc A. Doré, Hugo Haguait, Julie Pittera, Justine Conan, Maël Ratin, Morgane Corre, Erwan Le Corguillé, Gildas Brillet-Guéguen, Loraine Hoebeke, Mark Six, Christophe Steglich, Claudia Siegel, Anne Eveillard, Damien Partensky, Frédéric Garczarek, Laurence Front Microbiol Microbiology Understanding how microorganisms adjust their metabolism to maintain their ability to cope with short-term environmental variations constitutes one of the major current challenges in microbial ecology. Here, the best physiologically characterized marine Synechococcus strain, WH7803, was exposed to modulated light/dark cycles or acclimated to continuous high-light (HL) or low-light (LL), then shifted to various stress conditions, including low (LT) or high temperature (HT), HL and ultraviolet (UV) radiations. Physiological responses were analyzed by measuring time courses of photosystem (PS) II quantum yield, PSII repair rate, pigment ratios and global changes in gene expression. Previously published membrane lipid composition were also used for correlation analyses. These data revealed that cells previously acclimated to HL are better prepared than LL-acclimated cells to sustain an additional light or UV stress, but not a LT stress. Indeed, LT seems to induce a synergic effect with the HL treatment, as previously observed with oxidative stress. While all tested shift conditions induced the downregulation of many photosynthetic genes, notably those encoding PSI, cytochrome b(6)/f and phycobilisomes, UV stress proved to be more deleterious for PSII than the other treatments, and full recovery of damaged PSII from UV stress seemed to involve the neo-synthesis of a fairly large number of PSII subunits and not just the reassembly of pre-existing subunits after D1 replacement. In contrast, genes involved in glycogen degradation and carotenoid biosynthesis pathways were more particularly upregulated in response to LT. Altogether, these experiments allowed us to identify responses common to all stresses and those more specific to a given stress, thus highlighting genes potentially involved in niche acclimation of a key member of marine ecosystems. Our data also revealed important specific features of the stress responses compared to model freshwater cyanobacteria. Frontiers Media S.A. 2020-07-24 /pmc/articles/PMC7393227/ /pubmed/32793165 http://dx.doi.org/10.3389/fmicb.2020.01707 Text en Copyright © 2020 Guyet, Nguyen, Doré, Haguait, Pittera, Conan, Ratin, Corre, Le Corguillé, Brillet-Guéguen, Hoebeke, Six, Steglich, Siegel, Eveillard, Partensky and Garczarek. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Guyet, Ulysse
Nguyen, Ngoc A.
Doré, Hugo
Haguait, Julie
Pittera, Justine
Conan, Maël
Ratin, Morgane
Corre, Erwan
Le Corguillé, Gildas
Brillet-Guéguen, Loraine
Hoebeke, Mark
Six, Christophe
Steglich, Claudia
Siegel, Anne
Eveillard, Damien
Partensky, Frédéric
Garczarek, Laurence
Synergic Effects of Temperature and Irradiance on the Physiology of the Marine Synechococcus Strain WH7803
title Synergic Effects of Temperature and Irradiance on the Physiology of the Marine Synechococcus Strain WH7803
title_full Synergic Effects of Temperature and Irradiance on the Physiology of the Marine Synechococcus Strain WH7803
title_fullStr Synergic Effects of Temperature and Irradiance on the Physiology of the Marine Synechococcus Strain WH7803
title_full_unstemmed Synergic Effects of Temperature and Irradiance on the Physiology of the Marine Synechococcus Strain WH7803
title_short Synergic Effects of Temperature and Irradiance on the Physiology of the Marine Synechococcus Strain WH7803
title_sort synergic effects of temperature and irradiance on the physiology of the marine synechococcus strain wh7803
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7393227/
https://www.ncbi.nlm.nih.gov/pubmed/32793165
http://dx.doi.org/10.3389/fmicb.2020.01707
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