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Liquid biopsy by NGS: Differential presence of exons (DPE) is related to metastatic potential in a colon-cancer model in the rat
Differential presence of exons (DPE) is a method of interpretation of exome sequencing, which has been proposed to design a predictive algorithm with clinical value in patients with colorectal cancer (CRC). The goal of the present study was to examine the reproducibility in a rat model of metastatic...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Neoplasia Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7393324/ https://www.ncbi.nlm.nih.gov/pubmed/32736333 http://dx.doi.org/10.1016/j.tranon.2020.100837 |
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author | García-Olmo, Dolores C. Peiró-Pastor, Ramón Picazo, María G. Olmedillas-López, Susana García-Arranz, Mariano Aguado, Begoña García-Olmo, Damián |
author_facet | García-Olmo, Dolores C. Peiró-Pastor, Ramón Picazo, María G. Olmedillas-López, Susana García-Arranz, Mariano Aguado, Begoña García-Olmo, Damián |
author_sort | García-Olmo, Dolores C. |
collection | PubMed |
description | Differential presence of exons (DPE) is a method of interpretation of exome sequencing, which has been proposed to design a predictive algorithm with clinical value in patients with colorectal cancer (CRC). The goal of the present study was to examine the reproducibility in a rat model of metastatic colon cancer. DHD/K12-TRb cells were injected in syngenic immunocompetent BD-IX rats. Cells were from two stocks with low and normal metastatic potential, and injected into two separate groups of rats. Five to ten weeks after injection, blood samples were taken prior euthanasia and whole exome sequencing performed. Through DPE analysis, we identified a set of exons whose differential presence in plasma allowed us to compare both groups of tumor-bearing animals. A verification test was performed to confirm that the algorithm was able to classify extracted samples into their corresponding groups of origin. The highest mean probability was 0.8954. In conclusion, the DPE analysis in tumor-bearing animals was able to discriminate between different disease status, which fully supports previous results in CRC patients. |
format | Online Article Text |
id | pubmed-7393324 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Neoplasia Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-73933242020-08-06 Liquid biopsy by NGS: Differential presence of exons (DPE) is related to metastatic potential in a colon-cancer model in the rat García-Olmo, Dolores C. Peiró-Pastor, Ramón Picazo, María G. Olmedillas-López, Susana García-Arranz, Mariano Aguado, Begoña García-Olmo, Damián Transl Oncol Original article Differential presence of exons (DPE) is a method of interpretation of exome sequencing, which has been proposed to design a predictive algorithm with clinical value in patients with colorectal cancer (CRC). The goal of the present study was to examine the reproducibility in a rat model of metastatic colon cancer. DHD/K12-TRb cells were injected in syngenic immunocompetent BD-IX rats. Cells were from two stocks with low and normal metastatic potential, and injected into two separate groups of rats. Five to ten weeks after injection, blood samples were taken prior euthanasia and whole exome sequencing performed. Through DPE analysis, we identified a set of exons whose differential presence in plasma allowed us to compare both groups of tumor-bearing animals. A verification test was performed to confirm that the algorithm was able to classify extracted samples into their corresponding groups of origin. The highest mean probability was 0.8954. In conclusion, the DPE analysis in tumor-bearing animals was able to discriminate between different disease status, which fully supports previous results in CRC patients. Neoplasia Press 2020-07-28 /pmc/articles/PMC7393324/ /pubmed/32736333 http://dx.doi.org/10.1016/j.tranon.2020.100837 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original article García-Olmo, Dolores C. Peiró-Pastor, Ramón Picazo, María G. Olmedillas-López, Susana García-Arranz, Mariano Aguado, Begoña García-Olmo, Damián Liquid biopsy by NGS: Differential presence of exons (DPE) is related to metastatic potential in a colon-cancer model in the rat |
title | Liquid biopsy by NGS: Differential presence of exons (DPE) is related to metastatic potential in a colon-cancer model in the rat |
title_full | Liquid biopsy by NGS: Differential presence of exons (DPE) is related to metastatic potential in a colon-cancer model in the rat |
title_fullStr | Liquid biopsy by NGS: Differential presence of exons (DPE) is related to metastatic potential in a colon-cancer model in the rat |
title_full_unstemmed | Liquid biopsy by NGS: Differential presence of exons (DPE) is related to metastatic potential in a colon-cancer model in the rat |
title_short | Liquid biopsy by NGS: Differential presence of exons (DPE) is related to metastatic potential in a colon-cancer model in the rat |
title_sort | liquid biopsy by ngs: differential presence of exons (dpe) is related to metastatic potential in a colon-cancer model in the rat |
topic | Original article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7393324/ https://www.ncbi.nlm.nih.gov/pubmed/32736333 http://dx.doi.org/10.1016/j.tranon.2020.100837 |
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