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Comparing different sperm separation techniques for ART, through quantitative evaluation of p53 protein

CONTEXT: In the last 10 years, assisted reproductive technologies (ARTs) have offered infertile couples an opportunity to complete their reproductive project. However, the high failure rate could be explained with the complex human reproduction system. In ART, the decrease of the success is due to t...

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Autores principales: Raimondo, Salvatore, Gentile, Tommaso, Gentile, Mariacira, Donnarumma, Francesca, Esposito, Giusy, Morelli, Alessia, De Filippo, Stefania, Cuomo, Felice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7394090/
https://www.ncbi.nlm.nih.gov/pubmed/32792760
http://dx.doi.org/10.4103/jhrs.JHRS_117_19
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author Raimondo, Salvatore
Gentile, Tommaso
Gentile, Mariacira
Donnarumma, Francesca
Esposito, Giusy
Morelli, Alessia
De Filippo, Stefania
Cuomo, Felice
author_facet Raimondo, Salvatore
Gentile, Tommaso
Gentile, Mariacira
Donnarumma, Francesca
Esposito, Giusy
Morelli, Alessia
De Filippo, Stefania
Cuomo, Felice
author_sort Raimondo, Salvatore
collection PubMed
description CONTEXT: In the last 10 years, assisted reproductive technologies (ARTs) have offered infertile couples an opportunity to complete their reproductive project. However, the high failure rate could be explained with the complex human reproduction system. In ART, the decrease of the success is due to the conditions far from the natural ones. AIMS: The aim of this study is to evaluate deoxyribonucleic acid (DNA) damage of spermatozoa before and after selection procedures, using a new technique able to quantize sperm DNA damage. SETTINGS AND DESIGN: They were involved 43 males domiciled permanently in two areas with different Environmental Impact, HEI (high environmental impact) and LEI (Low environmental impact), they are aged between 24 and 31 years with various degrees of dyspermia. SUBJECTS AND METHODS: The 43 males were divided into two groups: 21 in Group A (EIL) and 22 in Group B (EIH). The samples must be aliquoted into parts of 0.5 mL: Group (a) Control, no processing; Group (b) Swim-up (SUP) from semen; Group (c) classic SUP; Group (d) density gradient centrifugation (DGC). All samples were subjected to a quantitative dosage of p53 protein, before and after processing. STATISTICAL ANALYSIS USED: For the development of the probability and significance of the data, the Student's t-test was used. RESULTS: From our data, it emerges that Groups D and B provide a superior quality about motility, vitality, and apoptosis indexes compared to other conventional techniques. In Group B, apoptosis is comparable to Group D, but they have slightly lower about motility and vitality. Group C is the one that has lower parameters than the other techniques. Regarding the evaluation of p53 protein, the results are conflicting with the evaluation of apoptosis; in fact, in Group D, the values are significantly higher than the other techniques. CONCLUSIONS: Sperm separation is an important moment in ART techniques. From our data, it emerges a greater fragility of DNA in the male spermatozoa who reside permanently in areas with high environmental impact.
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spelling pubmed-73940902020-08-12 Comparing different sperm separation techniques for ART, through quantitative evaluation of p53 protein Raimondo, Salvatore Gentile, Tommaso Gentile, Mariacira Donnarumma, Francesca Esposito, Giusy Morelli, Alessia De Filippo, Stefania Cuomo, Felice J Hum Reprod Sci Original Article CONTEXT: In the last 10 years, assisted reproductive technologies (ARTs) have offered infertile couples an opportunity to complete their reproductive project. However, the high failure rate could be explained with the complex human reproduction system. In ART, the decrease of the success is due to the conditions far from the natural ones. AIMS: The aim of this study is to evaluate deoxyribonucleic acid (DNA) damage of spermatozoa before and after selection procedures, using a new technique able to quantize sperm DNA damage. SETTINGS AND DESIGN: They were involved 43 males domiciled permanently in two areas with different Environmental Impact, HEI (high environmental impact) and LEI (Low environmental impact), they are aged between 24 and 31 years with various degrees of dyspermia. SUBJECTS AND METHODS: The 43 males were divided into two groups: 21 in Group A (EIL) and 22 in Group B (EIH). The samples must be aliquoted into parts of 0.5 mL: Group (a) Control, no processing; Group (b) Swim-up (SUP) from semen; Group (c) classic SUP; Group (d) density gradient centrifugation (DGC). All samples were subjected to a quantitative dosage of p53 protein, before and after processing. STATISTICAL ANALYSIS USED: For the development of the probability and significance of the data, the Student's t-test was used. RESULTS: From our data, it emerges that Groups D and B provide a superior quality about motility, vitality, and apoptosis indexes compared to other conventional techniques. In Group B, apoptosis is comparable to Group D, but they have slightly lower about motility and vitality. Group C is the one that has lower parameters than the other techniques. Regarding the evaluation of p53 protein, the results are conflicting with the evaluation of apoptosis; in fact, in Group D, the values are significantly higher than the other techniques. CONCLUSIONS: Sperm separation is an important moment in ART techniques. From our data, it emerges a greater fragility of DNA in the male spermatozoa who reside permanently in areas with high environmental impact. Wolters Kluwer - Medknow 2020 2020-07-09 /pmc/articles/PMC7394090/ /pubmed/32792760 http://dx.doi.org/10.4103/jhrs.JHRS_117_19 Text en Copyright: © 2020 Journal of Human Reproductive Sciences http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Raimondo, Salvatore
Gentile, Tommaso
Gentile, Mariacira
Donnarumma, Francesca
Esposito, Giusy
Morelli, Alessia
De Filippo, Stefania
Cuomo, Felice
Comparing different sperm separation techniques for ART, through quantitative evaluation of p53 protein
title Comparing different sperm separation techniques for ART, through quantitative evaluation of p53 protein
title_full Comparing different sperm separation techniques for ART, through quantitative evaluation of p53 protein
title_fullStr Comparing different sperm separation techniques for ART, through quantitative evaluation of p53 protein
title_full_unstemmed Comparing different sperm separation techniques for ART, through quantitative evaluation of p53 protein
title_short Comparing different sperm separation techniques for ART, through quantitative evaluation of p53 protein
title_sort comparing different sperm separation techniques for art, through quantitative evaluation of p53 protein
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7394090/
https://www.ncbi.nlm.nih.gov/pubmed/32792760
http://dx.doi.org/10.4103/jhrs.JHRS_117_19
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