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CYP19A1 Gene Expression in Patients with Polycystic Ovarian Syndrome
CONTEXT: Polycystic ovarian syndrome (PCOS) is a common endocrine system disorder among the women of reproductive age, yet the etiology of PCOS remains unclear. Infertility in females with PCOS can be caused by anovulation, high luteinizing hormone levels, and hyperandrogenism. AIMS: This research a...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7394102/ https://www.ncbi.nlm.nih.gov/pubmed/32792756 http://dx.doi.org/10.4103/jhrs.JHRS_142_18 |
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author | Panghiyangani, Roselina Soeharso, Purnomo Andrijono, Suryandari, Dwi Anita Wiweko, Budi Kurniati, Mala Pujianto, Dwi Ari |
author_facet | Panghiyangani, Roselina Soeharso, Purnomo Andrijono, Suryandari, Dwi Anita Wiweko, Budi Kurniati, Mala Pujianto, Dwi Ari |
author_sort | Panghiyangani, Roselina |
collection | PubMed |
description | CONTEXT: Polycystic ovarian syndrome (PCOS) is a common endocrine system disorder among the women of reproductive age, yet the etiology of PCOS remains unclear. Infertility in females with PCOS can be caused by anovulation, high luteinizing hormone levels, and hyperandrogenism. AIMS: This research analyzed the role of the aromatase gene (CYP19A1) in PCOS pathogenesis. SETTINGS AND DESIGN: This study used an observational, cross-sectional design. SUBJECTS AND METHODS: A total of 110 research participants (55 PCOS patients and 55 non-PCOS patients) were included in the study. STATISTICAL ANALYSIS USED: A real-time quantitative polymerase chain reaction was used to analyze the mRNA expression for aromatase in granulosa cells. RESULTS: The relative expression of aromatase mRNA is lower in women with PCOS compared to those without PCOS (P < 0.05). Relative expression of CYP19A1 (aromatase) mRNA in PCOS group was 0.38 ± 0.25, whereas in non-PCOS group was 1.00 ± 0.00. The decline in aromatase activity contributes to an increase in testosterone level. This condition has a role in hyperandrogenism which is a typical characteristic of PCOS women. Granulosa cells in polycystic ovary undergo disturbance in the development and cannot respond to follicle-stimulating hormone (FSH) stimulation. Lack of stimulation of FSH causes induction inadequacy to aromatase enzyme activity in the aromatization process. The decline in FSH activity is caused by various factors that are associated with typical characteristics of PCOS. CONCLUSIONS: There is a decrease in the relative expression rate of granulosa cells’ aromatase mRNA in women with PCOS compared to the non-PCOS. |
format | Online Article Text |
id | pubmed-7394102 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-73941022020-08-12 CYP19A1 Gene Expression in Patients with Polycystic Ovarian Syndrome Panghiyangani, Roselina Soeharso, Purnomo Andrijono, Suryandari, Dwi Anita Wiweko, Budi Kurniati, Mala Pujianto, Dwi Ari J Hum Reprod Sci Original Article CONTEXT: Polycystic ovarian syndrome (PCOS) is a common endocrine system disorder among the women of reproductive age, yet the etiology of PCOS remains unclear. Infertility in females with PCOS can be caused by anovulation, high luteinizing hormone levels, and hyperandrogenism. AIMS: This research analyzed the role of the aromatase gene (CYP19A1) in PCOS pathogenesis. SETTINGS AND DESIGN: This study used an observational, cross-sectional design. SUBJECTS AND METHODS: A total of 110 research participants (55 PCOS patients and 55 non-PCOS patients) were included in the study. STATISTICAL ANALYSIS USED: A real-time quantitative polymerase chain reaction was used to analyze the mRNA expression for aromatase in granulosa cells. RESULTS: The relative expression of aromatase mRNA is lower in women with PCOS compared to those without PCOS (P < 0.05). Relative expression of CYP19A1 (aromatase) mRNA in PCOS group was 0.38 ± 0.25, whereas in non-PCOS group was 1.00 ± 0.00. The decline in aromatase activity contributes to an increase in testosterone level. This condition has a role in hyperandrogenism which is a typical characteristic of PCOS women. Granulosa cells in polycystic ovary undergo disturbance in the development and cannot respond to follicle-stimulating hormone (FSH) stimulation. Lack of stimulation of FSH causes induction inadequacy to aromatase enzyme activity in the aromatization process. The decline in FSH activity is caused by various factors that are associated with typical characteristics of PCOS. CONCLUSIONS: There is a decrease in the relative expression rate of granulosa cells’ aromatase mRNA in women with PCOS compared to the non-PCOS. Wolters Kluwer - Medknow 2020 2020-07-09 /pmc/articles/PMC7394102/ /pubmed/32792756 http://dx.doi.org/10.4103/jhrs.JHRS_142_18 Text en Copyright: © 2020 Journal of Human Reproductive Sciences http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Panghiyangani, Roselina Soeharso, Purnomo Andrijono, Suryandari, Dwi Anita Wiweko, Budi Kurniati, Mala Pujianto, Dwi Ari CYP19A1 Gene Expression in Patients with Polycystic Ovarian Syndrome |
title | CYP19A1 Gene Expression in Patients with Polycystic Ovarian Syndrome |
title_full | CYP19A1 Gene Expression in Patients with Polycystic Ovarian Syndrome |
title_fullStr | CYP19A1 Gene Expression in Patients with Polycystic Ovarian Syndrome |
title_full_unstemmed | CYP19A1 Gene Expression in Patients with Polycystic Ovarian Syndrome |
title_short | CYP19A1 Gene Expression in Patients with Polycystic Ovarian Syndrome |
title_sort | cyp19a1 gene expression in patients with polycystic ovarian syndrome |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7394102/ https://www.ncbi.nlm.nih.gov/pubmed/32792756 http://dx.doi.org/10.4103/jhrs.JHRS_142_18 |
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