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Use of a NAT-based assay to improve the surveillance system and prevent transfusion-transmitted malaria in blood banks

BACKGROUND: Malaria can be transmitted by blood transfusion through donations collected from asymptomatic donors. Transfusion-transmitted malaria (TTM) poses a great risk to blood services worldwide. A good screening tool for Plasmodium spp. detection in blood banks must have a high sensitivity for...

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Autores principales: Rocha, Daniele, de Melo, Gisely Cardoso, Carneiro, José Marcelo Hipólito, Ribeiro, Marisa, Ribeiro, Sthefanie, de Godoy, Daniela Tupy, de Almeida, Anne Cristine Gomes, de Andrade, Elisabete Ferreira, Abrahim, Cláudia Maria de Moura, Fraiji, Nelson Abrahim, Ferreira, Antonio Gomes Pinto, Monteiro, Wuelton Marcelo, Brindeiro, Rodrigo, Tanuri, Amilcar, de Lacerda, Marcus Vinicius Guimarães, Alvarez, Patrícia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7395345/
https://www.ncbi.nlm.nih.gov/pubmed/32736625
http://dx.doi.org/10.1186/s12936-020-03345-y
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author Rocha, Daniele
de Melo, Gisely Cardoso
Carneiro, José Marcelo Hipólito
Ribeiro, Marisa
Ribeiro, Sthefanie
de Godoy, Daniela Tupy
de Almeida, Anne Cristine Gomes
de Andrade, Elisabete Ferreira
Abrahim, Cláudia Maria de Moura
Fraiji, Nelson Abrahim
Ferreira, Antonio Gomes Pinto
Monteiro, Wuelton Marcelo
Brindeiro, Rodrigo
Tanuri, Amilcar
de Lacerda, Marcus Vinicius Guimarães
Alvarez, Patrícia
author_facet Rocha, Daniele
de Melo, Gisely Cardoso
Carneiro, José Marcelo Hipólito
Ribeiro, Marisa
Ribeiro, Sthefanie
de Godoy, Daniela Tupy
de Almeida, Anne Cristine Gomes
de Andrade, Elisabete Ferreira
Abrahim, Cláudia Maria de Moura
Fraiji, Nelson Abrahim
Ferreira, Antonio Gomes Pinto
Monteiro, Wuelton Marcelo
Brindeiro, Rodrigo
Tanuri, Amilcar
de Lacerda, Marcus Vinicius Guimarães
Alvarez, Patrícia
author_sort Rocha, Daniele
collection PubMed
description BACKGROUND: Malaria can be transmitted by blood transfusion through donations collected from asymptomatic donors. Transfusion-transmitted malaria (TTM) poses a great risk to blood services worldwide. A good screening tool for Plasmodium spp. detection in blood banks must have a high sensitivity for prevention of TTM. However, in Brazilian blood banks, screening for malaria still relies on microscopy. METHODS: In Brazil, screening for human immunodeficiency virus type 1 (HIV), RNA/DNA for hepatitis C (HCV) and hepatitis B (HBV) viruses is mandatory for every blood donation and uses nucleic acid amplification testing (NAT). The aim of this study was to evaluate the inclusion of an assay for malaria to identify Plasmodium sp. from total nucleic acid (TNA; DNA/RNA) by targeting the 18S rRNA gene of the parasite. RESULTS: Considering the limitations of microscopy and the wide availability of the Brazilian NAT platform in the screening of blood units for HIV, HCV, and HBV, a molecular diagnostic tool was validated for detection of Plasmodium sp. in blood banks; a pilot study showed that using this novel NAT assay could reduce the risk of TTM. CONCLUSION: The prototype HIV/HCV/HBV/malaria NAT assay was effective in detecting infected candidate donors and has good prospects to be applied in routine screening for preventing TTM.
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spelling pubmed-73953452020-08-05 Use of a NAT-based assay to improve the surveillance system and prevent transfusion-transmitted malaria in blood banks Rocha, Daniele de Melo, Gisely Cardoso Carneiro, José Marcelo Hipólito Ribeiro, Marisa Ribeiro, Sthefanie de Godoy, Daniela Tupy de Almeida, Anne Cristine Gomes de Andrade, Elisabete Ferreira Abrahim, Cláudia Maria de Moura Fraiji, Nelson Abrahim Ferreira, Antonio Gomes Pinto Monteiro, Wuelton Marcelo Brindeiro, Rodrigo Tanuri, Amilcar de Lacerda, Marcus Vinicius Guimarães Alvarez, Patrícia Malar J Research BACKGROUND: Malaria can be transmitted by blood transfusion through donations collected from asymptomatic donors. Transfusion-transmitted malaria (TTM) poses a great risk to blood services worldwide. A good screening tool for Plasmodium spp. detection in blood banks must have a high sensitivity for prevention of TTM. However, in Brazilian blood banks, screening for malaria still relies on microscopy. METHODS: In Brazil, screening for human immunodeficiency virus type 1 (HIV), RNA/DNA for hepatitis C (HCV) and hepatitis B (HBV) viruses is mandatory for every blood donation and uses nucleic acid amplification testing (NAT). The aim of this study was to evaluate the inclusion of an assay for malaria to identify Plasmodium sp. from total nucleic acid (TNA; DNA/RNA) by targeting the 18S rRNA gene of the parasite. RESULTS: Considering the limitations of microscopy and the wide availability of the Brazilian NAT platform in the screening of blood units for HIV, HCV, and HBV, a molecular diagnostic tool was validated for detection of Plasmodium sp. in blood banks; a pilot study showed that using this novel NAT assay could reduce the risk of TTM. CONCLUSION: The prototype HIV/HCV/HBV/malaria NAT assay was effective in detecting infected candidate donors and has good prospects to be applied in routine screening for preventing TTM. BioMed Central 2020-07-31 /pmc/articles/PMC7395345/ /pubmed/32736625 http://dx.doi.org/10.1186/s12936-020-03345-y Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Rocha, Daniele
de Melo, Gisely Cardoso
Carneiro, José Marcelo Hipólito
Ribeiro, Marisa
Ribeiro, Sthefanie
de Godoy, Daniela Tupy
de Almeida, Anne Cristine Gomes
de Andrade, Elisabete Ferreira
Abrahim, Cláudia Maria de Moura
Fraiji, Nelson Abrahim
Ferreira, Antonio Gomes Pinto
Monteiro, Wuelton Marcelo
Brindeiro, Rodrigo
Tanuri, Amilcar
de Lacerda, Marcus Vinicius Guimarães
Alvarez, Patrícia
Use of a NAT-based assay to improve the surveillance system and prevent transfusion-transmitted malaria in blood banks
title Use of a NAT-based assay to improve the surveillance system and prevent transfusion-transmitted malaria in blood banks
title_full Use of a NAT-based assay to improve the surveillance system and prevent transfusion-transmitted malaria in blood banks
title_fullStr Use of a NAT-based assay to improve the surveillance system and prevent transfusion-transmitted malaria in blood banks
title_full_unstemmed Use of a NAT-based assay to improve the surveillance system and prevent transfusion-transmitted malaria in blood banks
title_short Use of a NAT-based assay to improve the surveillance system and prevent transfusion-transmitted malaria in blood banks
title_sort use of a nat-based assay to improve the surveillance system and prevent transfusion-transmitted malaria in blood banks
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7395345/
https://www.ncbi.nlm.nih.gov/pubmed/32736625
http://dx.doi.org/10.1186/s12936-020-03345-y
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