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Mammalian deltavirus without hepadnavirus coinfection in the neotropical rodent Proechimys semispinosus

Hepatitis delta virus (HDV) is a human hepatitis-causing RNA virus, unrelated to any other taxonomic group of RNA viruses. Its occurrence as a satellite virus of hepatitis B virus (HBV) is a singular case in animal virology for which no consensus evolutionary explanation exists. Here we present a ma...

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Autores principales: Paraskevopoulou, Sofia, Pirzer, Fabian, Goldmann, Nora, Schmid, Julian, Corman, Victor Max, Gottula, Lina Theresa, Schroeder, Simon, Rasche, Andrea, Muth, Doreen, Drexler, Jan Felix, Heni, Alexander Christoph, Eibner, Georg Joachim, Page, Rachel A., Jones, Terry C., Müller, Marcel A., Sommer, Simone, Glebe, Dieter, Drosten, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7395443/
https://www.ncbi.nlm.nih.gov/pubmed/32651267
http://dx.doi.org/10.1073/pnas.2006750117
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author Paraskevopoulou, Sofia
Pirzer, Fabian
Goldmann, Nora
Schmid, Julian
Corman, Victor Max
Gottula, Lina Theresa
Schroeder, Simon
Rasche, Andrea
Muth, Doreen
Drexler, Jan Felix
Heni, Alexander Christoph
Eibner, Georg Joachim
Page, Rachel A.
Jones, Terry C.
Müller, Marcel A.
Sommer, Simone
Glebe, Dieter
Drosten, Christian
author_facet Paraskevopoulou, Sofia
Pirzer, Fabian
Goldmann, Nora
Schmid, Julian
Corman, Victor Max
Gottula, Lina Theresa
Schroeder, Simon
Rasche, Andrea
Muth, Doreen
Drexler, Jan Felix
Heni, Alexander Christoph
Eibner, Georg Joachim
Page, Rachel A.
Jones, Terry C.
Müller, Marcel A.
Sommer, Simone
Glebe, Dieter
Drosten, Christian
author_sort Paraskevopoulou, Sofia
collection PubMed
description Hepatitis delta virus (HDV) is a human hepatitis-causing RNA virus, unrelated to any other taxonomic group of RNA viruses. Its occurrence as a satellite virus of hepatitis B virus (HBV) is a singular case in animal virology for which no consensus evolutionary explanation exists. Here we present a mammalian deltavirus that does not occur in humans, identified in the neotropical rodent species Proechimys semispinosus. The rodent deltavirus is highly distinct, showing a common ancestor with a recently described deltavirus in snakes. Reverse genetics based on a tandem minus-strand complementary DNA genome copy under the control of a cytomegalovirus (CMV) promoter confirms autonomous genome replication in transfected cells, with initiation of replication from the upstream genome copy. In contrast to HDV, a large delta antigen is not expressed and the farnesylation motif critical for HBV interaction is absent from a genome region that might correspond to a hypothetical rodent large delta antigen. Correspondingly, there is no evidence for coinfection with an HBV-related hepadnavirus based on virus detection and serology in any deltavirus-positive animal. No other coinfecting viruses were detected by RNA sequencing studies of 120 wild-caught animals that could serve as a potential helper virus. The presence of virus in blood and pronounced detection in reproductively active males suggest horizontal transmission linked to competitive behavior. Our study establishes a nonhuman, mammalian deltavirus that occurs as a horizontally transmitted infection, is potentially cleared by immune response, is not focused in the liver, and possibly does not require helper virus coinfection.
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spelling pubmed-73954432020-08-07 Mammalian deltavirus without hepadnavirus coinfection in the neotropical rodent Proechimys semispinosus Paraskevopoulou, Sofia Pirzer, Fabian Goldmann, Nora Schmid, Julian Corman, Victor Max Gottula, Lina Theresa Schroeder, Simon Rasche, Andrea Muth, Doreen Drexler, Jan Felix Heni, Alexander Christoph Eibner, Georg Joachim Page, Rachel A. Jones, Terry C. Müller, Marcel A. Sommer, Simone Glebe, Dieter Drosten, Christian Proc Natl Acad Sci U S A Biological Sciences Hepatitis delta virus (HDV) is a human hepatitis-causing RNA virus, unrelated to any other taxonomic group of RNA viruses. Its occurrence as a satellite virus of hepatitis B virus (HBV) is a singular case in animal virology for which no consensus evolutionary explanation exists. Here we present a mammalian deltavirus that does not occur in humans, identified in the neotropical rodent species Proechimys semispinosus. The rodent deltavirus is highly distinct, showing a common ancestor with a recently described deltavirus in snakes. Reverse genetics based on a tandem minus-strand complementary DNA genome copy under the control of a cytomegalovirus (CMV) promoter confirms autonomous genome replication in transfected cells, with initiation of replication from the upstream genome copy. In contrast to HDV, a large delta antigen is not expressed and the farnesylation motif critical for HBV interaction is absent from a genome region that might correspond to a hypothetical rodent large delta antigen. Correspondingly, there is no evidence for coinfection with an HBV-related hepadnavirus based on virus detection and serology in any deltavirus-positive animal. No other coinfecting viruses were detected by RNA sequencing studies of 120 wild-caught animals that could serve as a potential helper virus. The presence of virus in blood and pronounced detection in reproductively active males suggest horizontal transmission linked to competitive behavior. Our study establishes a nonhuman, mammalian deltavirus that occurs as a horizontally transmitted infection, is potentially cleared by immune response, is not focused in the liver, and possibly does not require helper virus coinfection. National Academy of Sciences 2020-07-28 2020-07-10 /pmc/articles/PMC7395443/ /pubmed/32651267 http://dx.doi.org/10.1073/pnas.2006750117 Text en Copyright © 2020 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ https://creativecommons.org/licenses/by-nc-nd/4.0/This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Biological Sciences
Paraskevopoulou, Sofia
Pirzer, Fabian
Goldmann, Nora
Schmid, Julian
Corman, Victor Max
Gottula, Lina Theresa
Schroeder, Simon
Rasche, Andrea
Muth, Doreen
Drexler, Jan Felix
Heni, Alexander Christoph
Eibner, Georg Joachim
Page, Rachel A.
Jones, Terry C.
Müller, Marcel A.
Sommer, Simone
Glebe, Dieter
Drosten, Christian
Mammalian deltavirus without hepadnavirus coinfection in the neotropical rodent Proechimys semispinosus
title Mammalian deltavirus without hepadnavirus coinfection in the neotropical rodent Proechimys semispinosus
title_full Mammalian deltavirus without hepadnavirus coinfection in the neotropical rodent Proechimys semispinosus
title_fullStr Mammalian deltavirus without hepadnavirus coinfection in the neotropical rodent Proechimys semispinosus
title_full_unstemmed Mammalian deltavirus without hepadnavirus coinfection in the neotropical rodent Proechimys semispinosus
title_short Mammalian deltavirus without hepadnavirus coinfection in the neotropical rodent Proechimys semispinosus
title_sort mammalian deltavirus without hepadnavirus coinfection in the neotropical rodent proechimys semispinosus
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7395443/
https://www.ncbi.nlm.nih.gov/pubmed/32651267
http://dx.doi.org/10.1073/pnas.2006750117
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