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Dynamic CCN3 expression in the murine CNS does not confer essential roles in myelination or remyelination

CCN3 is a matricellular protein that promotes oligodendrocyte progenitor cell differentiation and myelination in vitro and ex vivo. CCN3 is therefore a candidate of interest in central nervous system (CNS) myelination and remyelination, and we sought to investigate the expression and role of CCN3 du...

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Autores principales: de la Vega Gallardo, Nira, Penalva, Rosana, Dittmer, Marie, Naughton, Michelle, Falconer, John, Moffat, Jill, de la Fuente, Alerie G., Hombrebueno, José R., Lin, Zhiyong, Perbal, Bernard, Ingram, Rebecca J., Evergren, Emma, Fitzgerald, Denise C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7395501/
https://www.ncbi.nlm.nih.gov/pubmed/32651278
http://dx.doi.org/10.1073/pnas.1922089117
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author de la Vega Gallardo, Nira
Penalva, Rosana
Dittmer, Marie
Naughton, Michelle
Falconer, John
Moffat, Jill
de la Fuente, Alerie G.
Hombrebueno, José R.
Lin, Zhiyong
Perbal, Bernard
Ingram, Rebecca J.
Evergren, Emma
Fitzgerald, Denise C.
author_facet de la Vega Gallardo, Nira
Penalva, Rosana
Dittmer, Marie
Naughton, Michelle
Falconer, John
Moffat, Jill
de la Fuente, Alerie G.
Hombrebueno, José R.
Lin, Zhiyong
Perbal, Bernard
Ingram, Rebecca J.
Evergren, Emma
Fitzgerald, Denise C.
author_sort de la Vega Gallardo, Nira
collection PubMed
description CCN3 is a matricellular protein that promotes oligodendrocyte progenitor cell differentiation and myelination in vitro and ex vivo. CCN3 is therefore a candidate of interest in central nervous system (CNS) myelination and remyelination, and we sought to investigate the expression and role of CCN3 during these processes. We found CCN3 to be expressed predominantly by neurons in distinct areas of the CNS, primarily the cerebral cortex, hippocampus, amygdala, suprachiasmatic nuclei, anterior olfactory nuclei, and spinal cord gray matter. CCN3 was transiently up-regulated following demyelination in the brain of cuprizone-fed mice and spinal cord lesions of mice injected with lysolecithin. However, CCN3(−/−) mice did not exhibit significantly different numbers of oligodendroglia or differentiated oligodendrocytes in the healthy or remyelinating CNS, compared to WT controls. These results suggest that despite robust and dynamic expression in the CNS, CCN3 is not required for efficient myelination or remyelination in the murine CNS in vivo.
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spelling pubmed-73955012020-08-07 Dynamic CCN3 expression in the murine CNS does not confer essential roles in myelination or remyelination de la Vega Gallardo, Nira Penalva, Rosana Dittmer, Marie Naughton, Michelle Falconer, John Moffat, Jill de la Fuente, Alerie G. Hombrebueno, José R. Lin, Zhiyong Perbal, Bernard Ingram, Rebecca J. Evergren, Emma Fitzgerald, Denise C. Proc Natl Acad Sci U S A Biological Sciences CCN3 is a matricellular protein that promotes oligodendrocyte progenitor cell differentiation and myelination in vitro and ex vivo. CCN3 is therefore a candidate of interest in central nervous system (CNS) myelination and remyelination, and we sought to investigate the expression and role of CCN3 during these processes. We found CCN3 to be expressed predominantly by neurons in distinct areas of the CNS, primarily the cerebral cortex, hippocampus, amygdala, suprachiasmatic nuclei, anterior olfactory nuclei, and spinal cord gray matter. CCN3 was transiently up-regulated following demyelination in the brain of cuprizone-fed mice and spinal cord lesions of mice injected with lysolecithin. However, CCN3(−/−) mice did not exhibit significantly different numbers of oligodendroglia or differentiated oligodendrocytes in the healthy or remyelinating CNS, compared to WT controls. These results suggest that despite robust and dynamic expression in the CNS, CCN3 is not required for efficient myelination or remyelination in the murine CNS in vivo. National Academy of Sciences 2020-07-28 2020-07-10 /pmc/articles/PMC7395501/ /pubmed/32651278 http://dx.doi.org/10.1073/pnas.1922089117 Text en Copyright © 2020 the Author(s). Published by PNAS. http://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Biological Sciences
de la Vega Gallardo, Nira
Penalva, Rosana
Dittmer, Marie
Naughton, Michelle
Falconer, John
Moffat, Jill
de la Fuente, Alerie G.
Hombrebueno, José R.
Lin, Zhiyong
Perbal, Bernard
Ingram, Rebecca J.
Evergren, Emma
Fitzgerald, Denise C.
Dynamic CCN3 expression in the murine CNS does not confer essential roles in myelination or remyelination
title Dynamic CCN3 expression in the murine CNS does not confer essential roles in myelination or remyelination
title_full Dynamic CCN3 expression in the murine CNS does not confer essential roles in myelination or remyelination
title_fullStr Dynamic CCN3 expression in the murine CNS does not confer essential roles in myelination or remyelination
title_full_unstemmed Dynamic CCN3 expression in the murine CNS does not confer essential roles in myelination or remyelination
title_short Dynamic CCN3 expression in the murine CNS does not confer essential roles in myelination or remyelination
title_sort dynamic ccn3 expression in the murine cns does not confer essential roles in myelination or remyelination
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7395501/
https://www.ncbi.nlm.nih.gov/pubmed/32651278
http://dx.doi.org/10.1073/pnas.1922089117
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