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Early‐stage sugar beet taproot development is characterized by three distinct physiological phases

Despite the agronomic importance of sugar beet (Beta vulgaris L.), the early‐stage development of its taproot has only been poorly investigated. Thus, the mechanisms that determine growth and sugar accumulation in sugar beet are largely unknown. In the presented study, a physiological characterizati...

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Autores principales: Jammer, Alexandra, Albacete, Alfonso, Schulz, Britta, Koch, Wolfgang, Weltmeier, Fridtjof, van der Graaff, Eric, Pfeifhofer, Hartwig W., Roitsch, Thomas G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7395582/
https://www.ncbi.nlm.nih.gov/pubmed/32766510
http://dx.doi.org/10.1002/pld3.221
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author Jammer, Alexandra
Albacete, Alfonso
Schulz, Britta
Koch, Wolfgang
Weltmeier, Fridtjof
van der Graaff, Eric
Pfeifhofer, Hartwig W.
Roitsch, Thomas G.
author_facet Jammer, Alexandra
Albacete, Alfonso
Schulz, Britta
Koch, Wolfgang
Weltmeier, Fridtjof
van der Graaff, Eric
Pfeifhofer, Hartwig W.
Roitsch, Thomas G.
author_sort Jammer, Alexandra
collection PubMed
description Despite the agronomic importance of sugar beet (Beta vulgaris L.), the early‐stage development of its taproot has only been poorly investigated. Thus, the mechanisms that determine growth and sugar accumulation in sugar beet are largely unknown. In the presented study, a physiological characterization of early‐stage sugar beet taproot development was conducted. Activities were analyzed for fourteen key enzymes of carbohydrate metabolism in developing taproots over the first 80 days after sowing. In addition, we performed in situ localizations of selected carbohydrate‐metabolic enzyme activities, anatomical investigations, and quantifications of soluble carbohydrates, hexose phosphates, and phytohormones. Based on the accumulation dynamics of biomass and sucrose, as well as on anatomical parameters, the early phase of taproot development could be subdivided into three stages—prestorage, transition, secondary growth and sucrose accumulation stage—each of which was characterized by distinct metabolic and phytohormonal signatures. The enzyme activity signatures corresponding to these stages were also shown to be robustly reproducible in experiments conducted in two additional locations. The results from this physiological phenotyping approach contribute to the identification of the key regulators of sugar beet taproot development and open up new perspectives for sugar beet crop improvement concerning both physiological marker‐based breeding and biotechnological approaches.
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spelling pubmed-73955822020-08-05 Early‐stage sugar beet taproot development is characterized by three distinct physiological phases Jammer, Alexandra Albacete, Alfonso Schulz, Britta Koch, Wolfgang Weltmeier, Fridtjof van der Graaff, Eric Pfeifhofer, Hartwig W. Roitsch, Thomas G. Plant Direct Original Research Despite the agronomic importance of sugar beet (Beta vulgaris L.), the early‐stage development of its taproot has only been poorly investigated. Thus, the mechanisms that determine growth and sugar accumulation in sugar beet are largely unknown. In the presented study, a physiological characterization of early‐stage sugar beet taproot development was conducted. Activities were analyzed for fourteen key enzymes of carbohydrate metabolism in developing taproots over the first 80 days after sowing. In addition, we performed in situ localizations of selected carbohydrate‐metabolic enzyme activities, anatomical investigations, and quantifications of soluble carbohydrates, hexose phosphates, and phytohormones. Based on the accumulation dynamics of biomass and sucrose, as well as on anatomical parameters, the early phase of taproot development could be subdivided into three stages—prestorage, transition, secondary growth and sucrose accumulation stage—each of which was characterized by distinct metabolic and phytohormonal signatures. The enzyme activity signatures corresponding to these stages were also shown to be robustly reproducible in experiments conducted in two additional locations. The results from this physiological phenotyping approach contribute to the identification of the key regulators of sugar beet taproot development and open up new perspectives for sugar beet crop improvement concerning both physiological marker‐based breeding and biotechnological approaches. John Wiley and Sons Inc. 2020-08-01 /pmc/articles/PMC7395582/ /pubmed/32766510 http://dx.doi.org/10.1002/pld3.221 Text en © 2020 The Authors. Plant Direct published by American Society of Plant Biologists, Society for Experimental Biology and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Research
Jammer, Alexandra
Albacete, Alfonso
Schulz, Britta
Koch, Wolfgang
Weltmeier, Fridtjof
van der Graaff, Eric
Pfeifhofer, Hartwig W.
Roitsch, Thomas G.
Early‐stage sugar beet taproot development is characterized by three distinct physiological phases
title Early‐stage sugar beet taproot development is characterized by three distinct physiological phases
title_full Early‐stage sugar beet taproot development is characterized by three distinct physiological phases
title_fullStr Early‐stage sugar beet taproot development is characterized by three distinct physiological phases
title_full_unstemmed Early‐stage sugar beet taproot development is characterized by three distinct physiological phases
title_short Early‐stage sugar beet taproot development is characterized by three distinct physiological phases
title_sort early‐stage sugar beet taproot development is characterized by three distinct physiological phases
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7395582/
https://www.ncbi.nlm.nih.gov/pubmed/32766510
http://dx.doi.org/10.1002/pld3.221
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