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Parallel reaction monitoring revealed tolerance to drought proteins in weedy rice (Oryza sativa f. spontanea)
Drought is a complicated abiotic stress factor with severe effects on rice growth and production. Weedy rice is a valuable genetic resource that possesses a strong capacity for drought tolerance, cold tolerance, and salt tolerance, and is an excellent material for studying rice tolerance. Here, acco...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7395730/ https://www.ncbi.nlm.nih.gov/pubmed/32737338 http://dx.doi.org/10.1038/s41598-020-69739-9 |
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author | Han, Bing Ma, Xiaoding Cui, Di Geng, Leiyue Cao, Guilan Zhang, Hui Han, Longzhi |
author_facet | Han, Bing Ma, Xiaoding Cui, Di Geng, Leiyue Cao, Guilan Zhang, Hui Han, Longzhi |
author_sort | Han, Bing |
collection | PubMed |
description | Drought is a complicated abiotic stress factor with severe effects on rice growth and production. Weedy rice is a valuable genetic resource that possesses a strong capacity for drought tolerance, cold tolerance, and salt tolerance, and is an excellent material for studying rice tolerance. Here, according to comprehensive tolerance to drought index D, accession WR16 was selected based on strong drought tolerance among 133 studied weedy red rice germplasms. WR16 was compared with Oryza sativa ssp. Japonica. cv. IAPAR-9, a reference genotype originating from Brazil. In addition, accession WR24 was classified as moderately tolerant to drought accessions. Transcriptomic and proteomic analyses were combined to identify 38 co-upregulated proteins related to drought tolerance, and targeted parallel reaction monitoring (PRM) was used to precisely quantify and verify nine proteins in the complex backgrounds. Result showed that six proteins were significantly (Fisher's exact P value < 0.05) related to drought tolerance in accessions WR16 and WR24. Among them, OS09T0478300-01, OS09T0530300-01, and OS01T0800500-01 formed a combined defense system to respond to drought stress in weedy rice. Results of these studies provide comprehensive information for precisely identifying and verifying tolerance to drought proteins and lay a solid theoretical foundation for research on drought tolerance mechanisms. |
format | Online Article Text |
id | pubmed-7395730 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-73957302020-08-04 Parallel reaction monitoring revealed tolerance to drought proteins in weedy rice (Oryza sativa f. spontanea) Han, Bing Ma, Xiaoding Cui, Di Geng, Leiyue Cao, Guilan Zhang, Hui Han, Longzhi Sci Rep Article Drought is a complicated abiotic stress factor with severe effects on rice growth and production. Weedy rice is a valuable genetic resource that possesses a strong capacity for drought tolerance, cold tolerance, and salt tolerance, and is an excellent material for studying rice tolerance. Here, according to comprehensive tolerance to drought index D, accession WR16 was selected based on strong drought tolerance among 133 studied weedy red rice germplasms. WR16 was compared with Oryza sativa ssp. Japonica. cv. IAPAR-9, a reference genotype originating from Brazil. In addition, accession WR24 was classified as moderately tolerant to drought accessions. Transcriptomic and proteomic analyses were combined to identify 38 co-upregulated proteins related to drought tolerance, and targeted parallel reaction monitoring (PRM) was used to precisely quantify and verify nine proteins in the complex backgrounds. Result showed that six proteins were significantly (Fisher's exact P value < 0.05) related to drought tolerance in accessions WR16 and WR24. Among them, OS09T0478300-01, OS09T0530300-01, and OS01T0800500-01 formed a combined defense system to respond to drought stress in weedy rice. Results of these studies provide comprehensive information for precisely identifying and verifying tolerance to drought proteins and lay a solid theoretical foundation for research on drought tolerance mechanisms. Nature Publishing Group UK 2020-07-31 /pmc/articles/PMC7395730/ /pubmed/32737338 http://dx.doi.org/10.1038/s41598-020-69739-9 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Han, Bing Ma, Xiaoding Cui, Di Geng, Leiyue Cao, Guilan Zhang, Hui Han, Longzhi Parallel reaction monitoring revealed tolerance to drought proteins in weedy rice (Oryza sativa f. spontanea) |
title | Parallel reaction monitoring revealed tolerance to drought proteins in weedy rice (Oryza sativa f. spontanea) |
title_full | Parallel reaction monitoring revealed tolerance to drought proteins in weedy rice (Oryza sativa f. spontanea) |
title_fullStr | Parallel reaction monitoring revealed tolerance to drought proteins in weedy rice (Oryza sativa f. spontanea) |
title_full_unstemmed | Parallel reaction monitoring revealed tolerance to drought proteins in weedy rice (Oryza sativa f. spontanea) |
title_short | Parallel reaction monitoring revealed tolerance to drought proteins in weedy rice (Oryza sativa f. spontanea) |
title_sort | parallel reaction monitoring revealed tolerance to drought proteins in weedy rice (oryza sativa f. spontanea) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7395730/ https://www.ncbi.nlm.nih.gov/pubmed/32737338 http://dx.doi.org/10.1038/s41598-020-69739-9 |
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