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Expression profiling of human milk derived exosomal microRNAs and their targets in HIV-1 infected mothers

Despite the use of antiretroviral therapy (ART) in HIV-1 infected mothers approximately 5% of new HIV-1 infections still occur in breastfed infants annually, which warrants for the development of novel strategies to prevent new HIV-1 infections in infants. Human milk (HM) exosomes are highly enriche...

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Autores principales: Zahoor, Muhammad Atif, Yao, Xiao-Dan, Henrick, Bethany M., Verschoor, Chris P., Abimiku, Alash’le, Osawe, Sophia, Rosenthal, Kenneth L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7395778/
https://www.ncbi.nlm.nih.gov/pubmed/32737406
http://dx.doi.org/10.1038/s41598-020-69799-x
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author Zahoor, Muhammad Atif
Yao, Xiao-Dan
Henrick, Bethany M.
Verschoor, Chris P.
Abimiku, Alash’le
Osawe, Sophia
Rosenthal, Kenneth L.
author_facet Zahoor, Muhammad Atif
Yao, Xiao-Dan
Henrick, Bethany M.
Verschoor, Chris P.
Abimiku, Alash’le
Osawe, Sophia
Rosenthal, Kenneth L.
author_sort Zahoor, Muhammad Atif
collection PubMed
description Despite the use of antiretroviral therapy (ART) in HIV-1 infected mothers approximately 5% of new HIV-1 infections still occur in breastfed infants annually, which warrants for the development of novel strategies to prevent new HIV-1 infections in infants. Human milk (HM) exosomes are highly enriched in microRNAs (miRNAs), which play an important role in neonatal immunity. Furthermore, HM exosomes from healthy donors are known to inhibit HIV-1 infection and transmission; however, the effect of HIV-1 on HM exosomal miRNA signatures remains unknown. In this study, we used nCounter NanoString technology and investigated miRNAs expression profiles in first week postpartum HM exosomes from HIV-1 infected and uninfected control mothers (n = 36). Our results indicated that HIV-1 perturbed the differential expression patterns of 19 miRNAs (13 upregulated and 6 downregulated) in HIV-1 infected women compared to healthy controls. DIANA-miR functional pathway analyses revealed that multiple biological pathways are involved including cell cycle, pathways in cancer, TGF-β signaling, FoxO signaling, fatty acid biosynthesis, p53 signaling and apoptosis. Moreover, the receiver operating characteristics (ROC) curve analyses of miR-630 and miR-378g yielded areas under the ROC curves of 0.82 (95% CI 0.67 to 0.82) and 0.83 (95% CI 0.67 to 0.83), respectively highlighting their potential to serve as biomarkers to identify HIV-1 infection in women. These data may contribute to the development of new therapeutic strategies in prevention of mother-to-child transmission (MTCT) of HIV-1.
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spelling pubmed-73957782020-08-04 Expression profiling of human milk derived exosomal microRNAs and their targets in HIV-1 infected mothers Zahoor, Muhammad Atif Yao, Xiao-Dan Henrick, Bethany M. Verschoor, Chris P. Abimiku, Alash’le Osawe, Sophia Rosenthal, Kenneth L. Sci Rep Article Despite the use of antiretroviral therapy (ART) in HIV-1 infected mothers approximately 5% of new HIV-1 infections still occur in breastfed infants annually, which warrants for the development of novel strategies to prevent new HIV-1 infections in infants. Human milk (HM) exosomes are highly enriched in microRNAs (miRNAs), which play an important role in neonatal immunity. Furthermore, HM exosomes from healthy donors are known to inhibit HIV-1 infection and transmission; however, the effect of HIV-1 on HM exosomal miRNA signatures remains unknown. In this study, we used nCounter NanoString technology and investigated miRNAs expression profiles in first week postpartum HM exosomes from HIV-1 infected and uninfected control mothers (n = 36). Our results indicated that HIV-1 perturbed the differential expression patterns of 19 miRNAs (13 upregulated and 6 downregulated) in HIV-1 infected women compared to healthy controls. DIANA-miR functional pathway analyses revealed that multiple biological pathways are involved including cell cycle, pathways in cancer, TGF-β signaling, FoxO signaling, fatty acid biosynthesis, p53 signaling and apoptosis. Moreover, the receiver operating characteristics (ROC) curve analyses of miR-630 and miR-378g yielded areas under the ROC curves of 0.82 (95% CI 0.67 to 0.82) and 0.83 (95% CI 0.67 to 0.83), respectively highlighting their potential to serve as biomarkers to identify HIV-1 infection in women. These data may contribute to the development of new therapeutic strategies in prevention of mother-to-child transmission (MTCT) of HIV-1. Nature Publishing Group UK 2020-07-31 /pmc/articles/PMC7395778/ /pubmed/32737406 http://dx.doi.org/10.1038/s41598-020-69799-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zahoor, Muhammad Atif
Yao, Xiao-Dan
Henrick, Bethany M.
Verschoor, Chris P.
Abimiku, Alash’le
Osawe, Sophia
Rosenthal, Kenneth L.
Expression profiling of human milk derived exosomal microRNAs and their targets in HIV-1 infected mothers
title Expression profiling of human milk derived exosomal microRNAs and their targets in HIV-1 infected mothers
title_full Expression profiling of human milk derived exosomal microRNAs and their targets in HIV-1 infected mothers
title_fullStr Expression profiling of human milk derived exosomal microRNAs and their targets in HIV-1 infected mothers
title_full_unstemmed Expression profiling of human milk derived exosomal microRNAs and their targets in HIV-1 infected mothers
title_short Expression profiling of human milk derived exosomal microRNAs and their targets in HIV-1 infected mothers
title_sort expression profiling of human milk derived exosomal micrornas and their targets in hiv-1 infected mothers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7395778/
https://www.ncbi.nlm.nih.gov/pubmed/32737406
http://dx.doi.org/10.1038/s41598-020-69799-x
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