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A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering
Reporter systems are routinely used in plant genetic engineering and functional genomics research. Most such plant reporter systems cause accumulation of foreign proteins. Here, we demonstrate a protein-independent reporter system, 3WJ-4 × Bro, based on a fluorescent RNA aptamer. Via transient expre...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7395781/ https://www.ncbi.nlm.nih.gov/pubmed/32737299 http://dx.doi.org/10.1038/s41467-020-17497-7 |
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author | Bai, Jiuyuan Luo, Yao Wang, Xin Li, Shi Luo, Mei Yin, Meng Zuo, Yuanli Li, Guolin Yao, Junyu Yang, Hua Zhang, Mingdi Wei, Wei Wang, Maolin Wang, Rui Fan, Chunhai Zhao, Yun |
author_facet | Bai, Jiuyuan Luo, Yao Wang, Xin Li, Shi Luo, Mei Yin, Meng Zuo, Yuanli Li, Guolin Yao, Junyu Yang, Hua Zhang, Mingdi Wei, Wei Wang, Maolin Wang, Rui Fan, Chunhai Zhao, Yun |
author_sort | Bai, Jiuyuan |
collection | PubMed |
description | Reporter systems are routinely used in plant genetic engineering and functional genomics research. Most such plant reporter systems cause accumulation of foreign proteins. Here, we demonstrate a protein-independent reporter system, 3WJ-4 × Bro, based on a fluorescent RNA aptamer. Via transient expression assays in both Escherichia coli and Nicotiana benthamiana, we show that 3WJ-4 × Bro is suitable for transgene identification and as an mRNA reporter for expression pattern analysis. Following stable transformation in Arabidopsis thaliana, 3WJ-4 × Bro co-segregates and co-expresses with target transcripts and is stably inherited through multiple generations. Further, 3WJ-4 × Bro can be used to visualize virus-mediated RNA delivery in plants. This study demonstrates a protein-independent reporter system that can be used for transgene identification and in vivo dynamic analysis of mRNA. |
format | Online Article Text |
id | pubmed-7395781 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-73957812020-08-18 A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering Bai, Jiuyuan Luo, Yao Wang, Xin Li, Shi Luo, Mei Yin, Meng Zuo, Yuanli Li, Guolin Yao, Junyu Yang, Hua Zhang, Mingdi Wei, Wei Wang, Maolin Wang, Rui Fan, Chunhai Zhao, Yun Nat Commun Article Reporter systems are routinely used in plant genetic engineering and functional genomics research. Most such plant reporter systems cause accumulation of foreign proteins. Here, we demonstrate a protein-independent reporter system, 3WJ-4 × Bro, based on a fluorescent RNA aptamer. Via transient expression assays in both Escherichia coli and Nicotiana benthamiana, we show that 3WJ-4 × Bro is suitable for transgene identification and as an mRNA reporter for expression pattern analysis. Following stable transformation in Arabidopsis thaliana, 3WJ-4 × Bro co-segregates and co-expresses with target transcripts and is stably inherited through multiple generations. Further, 3WJ-4 × Bro can be used to visualize virus-mediated RNA delivery in plants. This study demonstrates a protein-independent reporter system that can be used for transgene identification and in vivo dynamic analysis of mRNA. Nature Publishing Group UK 2020-07-31 /pmc/articles/PMC7395781/ /pubmed/32737299 http://dx.doi.org/10.1038/s41467-020-17497-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Bai, Jiuyuan Luo, Yao Wang, Xin Li, Shi Luo, Mei Yin, Meng Zuo, Yuanli Li, Guolin Yao, Junyu Yang, Hua Zhang, Mingdi Wei, Wei Wang, Maolin Wang, Rui Fan, Chunhai Zhao, Yun A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering |
title | A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering |
title_full | A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering |
title_fullStr | A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering |
title_full_unstemmed | A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering |
title_short | A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering |
title_sort | protein-independent fluorescent rna aptamer reporter system for plant genetic engineering |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7395781/ https://www.ncbi.nlm.nih.gov/pubmed/32737299 http://dx.doi.org/10.1038/s41467-020-17497-7 |
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