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Characterization and Lytic Activity of Isolated Escherichia coli Bacteriophages against Escherichia coli in vitro
BACKGROUND: Escherichia coli (E. coli) is the most common cause of urinary tract infection (UTI) and typically treated with antibiotics. Unrestricted use of antibiotics may lead to the emergence of antibiotic-resistant bacteria. The present study aimed to isolate and characterize phages against E. c...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Shiraz University of Medical Sciences
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7395952/ https://www.ncbi.nlm.nih.gov/pubmed/32801419 http://dx.doi.org/10.30476/ijms.2019.45420 |
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author | Rahimzadeh, Golnar Resch, Gregory Rezai, Mohammad Sadegh Nemati Hevelaee, Ebrahim |
author_facet | Rahimzadeh, Golnar Resch, Gregory Rezai, Mohammad Sadegh Nemati Hevelaee, Ebrahim |
author_sort | Rahimzadeh, Golnar |
collection | PubMed |
description | BACKGROUND: Escherichia coli (E. coli) is the most common cause of urinary tract infection (UTI) and typically treated with antibiotics. Unrestricted use of antibiotics may lead to the emergence of antibiotic-resistant bacteria. The present study aimed to isolate and characterize phages against E. coli from infected urine samples and to determine the lytic activity of phages against E. coli in vitro. METHODS: The present experimental study was conducted in the Laboratory of Bouali Sina Hospital (Sari, Iran) in May 2018. E. coli was identified from nine urine samples of patients with UTI using conventional microbiological methods. Bacteriophages were isolated from the infected urine specimens, and their lytic activity was determined using the spot test. The titer of the bacteriophages was measured using the double-layer agar technique. The morphology of the bacteriophages was revealed using transmission electron microscopy, and the latent time period and burst size were determined. Data were analyzed using the SPSS software package. RESULTS: E. coli was isolated from nine infected urine samples. The lytic activity of bacteriophages against E. coli was determined using the spot test by observing the formation of inhibition zones. Transmission electron microscopy showed E. coli phages belonging to the Myoviridae family. The latent time period was 20 minutes with a burst size of 1,200 plaque-forming unit (PFU) per infected cell. The results of the double-layer agar assay showed that the titer of bacteriophages was 20×10(8) PFU/mL. CONCLUSION: The E. coli bacteriophage was isolated from infected urine samples and characterized, and their lytic activity against E. coli was determined in vitro. |
format | Online Article Text |
id | pubmed-7395952 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Shiraz University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-73959522020-08-13 Characterization and Lytic Activity of Isolated Escherichia coli Bacteriophages against Escherichia coli in vitro Rahimzadeh, Golnar Resch, Gregory Rezai, Mohammad Sadegh Nemati Hevelaee, Ebrahim Iran J Med Sci Original Article BACKGROUND: Escherichia coli (E. coli) is the most common cause of urinary tract infection (UTI) and typically treated with antibiotics. Unrestricted use of antibiotics may lead to the emergence of antibiotic-resistant bacteria. The present study aimed to isolate and characterize phages against E. coli from infected urine samples and to determine the lytic activity of phages against E. coli in vitro. METHODS: The present experimental study was conducted in the Laboratory of Bouali Sina Hospital (Sari, Iran) in May 2018. E. coli was identified from nine urine samples of patients with UTI using conventional microbiological methods. Bacteriophages were isolated from the infected urine specimens, and their lytic activity was determined using the spot test. The titer of the bacteriophages was measured using the double-layer agar technique. The morphology of the bacteriophages was revealed using transmission electron microscopy, and the latent time period and burst size were determined. Data were analyzed using the SPSS software package. RESULTS: E. coli was isolated from nine infected urine samples. The lytic activity of bacteriophages against E. coli was determined using the spot test by observing the formation of inhibition zones. Transmission electron microscopy showed E. coli phages belonging to the Myoviridae family. The latent time period was 20 minutes with a burst size of 1,200 plaque-forming unit (PFU) per infected cell. The results of the double-layer agar assay showed that the titer of bacteriophages was 20×10(8) PFU/mL. CONCLUSION: The E. coli bacteriophage was isolated from infected urine samples and characterized, and their lytic activity against E. coli was determined in vitro. Shiraz University of Medical Sciences 2020-07 /pmc/articles/PMC7395952/ /pubmed/32801419 http://dx.doi.org/10.30476/ijms.2019.45420 Text en Copyright: © Iranian Journal of Medical Sciences http://creativecommons.org/licenses/by-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 4.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Rahimzadeh, Golnar Resch, Gregory Rezai, Mohammad Sadegh Nemati Hevelaee, Ebrahim Characterization and Lytic Activity of Isolated Escherichia coli Bacteriophages against Escherichia coli in vitro |
title | Characterization and Lytic Activity of Isolated Escherichia coli Bacteriophages against Escherichia coli in vitro |
title_full | Characterization and Lytic Activity of Isolated Escherichia coli Bacteriophages against Escherichia coli in vitro |
title_fullStr | Characterization and Lytic Activity of Isolated Escherichia coli Bacteriophages against Escherichia coli in vitro |
title_full_unstemmed | Characterization and Lytic Activity of Isolated Escherichia coli Bacteriophages against Escherichia coli in vitro |
title_short | Characterization and Lytic Activity of Isolated Escherichia coli Bacteriophages against Escherichia coli in vitro |
title_sort | characterization and lytic activity of isolated escherichia coli bacteriophages against escherichia coli in vitro |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7395952/ https://www.ncbi.nlm.nih.gov/pubmed/32801419 http://dx.doi.org/10.30476/ijms.2019.45420 |
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