A general method to optimize and functionalize red-shifted rhodamine dyes

Expanding the palette of fluorescent dyes is vital to push the frontier of biological imaging. Although rhodamine dyes remain the premier type of small-molecule fluorophore due to their bioavailability and brightness, variants excited with far-red or near-infrared light suffer from poor performance...

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Autores principales: Grimm, Jonathan B., Tkachuk, Ariana N., Xie, Liangqi, Choi, Heejun, Mohar, Boaz, Falco, Natalie, Schaefer, Kathy, Patel, Ronak, Zheng, Qinsi, Liu, Zhe, Lippincott-Schwartz, Jennifer, Brown, Timothy A., Lavis, Luke D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7396317/
https://www.ncbi.nlm.nih.gov/pubmed/32719532
http://dx.doi.org/10.1038/s41592-020-0909-6
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author Grimm, Jonathan B.
Tkachuk, Ariana N.
Xie, Liangqi
Choi, Heejun
Mohar, Boaz
Falco, Natalie
Schaefer, Kathy
Patel, Ronak
Zheng, Qinsi
Liu, Zhe
Lippincott-Schwartz, Jennifer
Brown, Timothy A.
Lavis, Luke D.
author_facet Grimm, Jonathan B.
Tkachuk, Ariana N.
Xie, Liangqi
Choi, Heejun
Mohar, Boaz
Falco, Natalie
Schaefer, Kathy
Patel, Ronak
Zheng, Qinsi
Liu, Zhe
Lippincott-Schwartz, Jennifer
Brown, Timothy A.
Lavis, Luke D.
author_sort Grimm, Jonathan B.
collection PubMed
description Expanding the palette of fluorescent dyes is vital to push the frontier of biological imaging. Although rhodamine dyes remain the premier type of small-molecule fluorophore due to their bioavailability and brightness, variants excited with far-red or near-infrared light suffer from poor performance due to their propensity to adopt a lipophilic, nonfluorescent form. We report a framework for rationalizing rhodamine behavior in biological environments and a general chemical modification for rhodamines that optimizes long-wavelength variants and enables facile functionalization with different chemical groups.
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spelling pubmed-73963172021-01-27 A general method to optimize and functionalize red-shifted rhodamine dyes Grimm, Jonathan B. Tkachuk, Ariana N. Xie, Liangqi Choi, Heejun Mohar, Boaz Falco, Natalie Schaefer, Kathy Patel, Ronak Zheng, Qinsi Liu, Zhe Lippincott-Schwartz, Jennifer Brown, Timothy A. Lavis, Luke D. Nat Methods Article Expanding the palette of fluorescent dyes is vital to push the frontier of biological imaging. Although rhodamine dyes remain the premier type of small-molecule fluorophore due to their bioavailability and brightness, variants excited with far-red or near-infrared light suffer from poor performance due to their propensity to adopt a lipophilic, nonfluorescent form. We report a framework for rationalizing rhodamine behavior in biological environments and a general chemical modification for rhodamines that optimizes long-wavelength variants and enables facile functionalization with different chemical groups. 2020-07-27 2020-08 /pmc/articles/PMC7396317/ /pubmed/32719532 http://dx.doi.org/10.1038/s41592-020-0909-6 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Grimm, Jonathan B.
Tkachuk, Ariana N.
Xie, Liangqi
Choi, Heejun
Mohar, Boaz
Falco, Natalie
Schaefer, Kathy
Patel, Ronak
Zheng, Qinsi
Liu, Zhe
Lippincott-Schwartz, Jennifer
Brown, Timothy A.
Lavis, Luke D.
A general method to optimize and functionalize red-shifted rhodamine dyes
title A general method to optimize and functionalize red-shifted rhodamine dyes
title_full A general method to optimize and functionalize red-shifted rhodamine dyes
title_fullStr A general method to optimize and functionalize red-shifted rhodamine dyes
title_full_unstemmed A general method to optimize and functionalize red-shifted rhodamine dyes
title_short A general method to optimize and functionalize red-shifted rhodamine dyes
title_sort general method to optimize and functionalize red-shifted rhodamine dyes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7396317/
https://www.ncbi.nlm.nih.gov/pubmed/32719532
http://dx.doi.org/10.1038/s41592-020-0909-6
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