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Echolocating Whales and Bats Express the Motor Protein Prestin in the Inner Ear: A Potential Marker for Hearing Loss

Prestin is an integral membrane motor protein located in outer hair cells of the mammalian cochlea. It is responsible for electromotility and required for cochlear amplification. Although prestin works in a cycle-by-cycle mode up to frequencies of at least 79 kHz, it is not known whether or not pres...

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Autores principales: Morell, Maria, Vogl, A. Wayne, IJsseldijk, Lonneke L., Piscitelli-Doshkov, Marina, Tong, Ling, Ostertag, Sonja, Ferreira, Marisa, Fraija-Fernandez, Natalia, Colegrove, Kathleen M., Puel, Jean-Luc, Raverty, Stephen A., Shadwick, Robert E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7396497/
https://www.ncbi.nlm.nih.gov/pubmed/32851016
http://dx.doi.org/10.3389/fvets.2020.00429
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author Morell, Maria
Vogl, A. Wayne
IJsseldijk, Lonneke L.
Piscitelli-Doshkov, Marina
Tong, Ling
Ostertag, Sonja
Ferreira, Marisa
Fraija-Fernandez, Natalia
Colegrove, Kathleen M.
Puel, Jean-Luc
Raverty, Stephen A.
Shadwick, Robert E.
author_facet Morell, Maria
Vogl, A. Wayne
IJsseldijk, Lonneke L.
Piscitelli-Doshkov, Marina
Tong, Ling
Ostertag, Sonja
Ferreira, Marisa
Fraija-Fernandez, Natalia
Colegrove, Kathleen M.
Puel, Jean-Luc
Raverty, Stephen A.
Shadwick, Robert E.
author_sort Morell, Maria
collection PubMed
description Prestin is an integral membrane motor protein located in outer hair cells of the mammalian cochlea. It is responsible for electromotility and required for cochlear amplification. Although prestin works in a cycle-by-cycle mode up to frequencies of at least 79 kHz, it is not known whether or not prestin is required for the extreme high frequencies used by echolocating species. Cetaceans are known to possess a prestin coding gene. However, the expression and distribution pattern of the protein in the cetacean cochlea has not been determined, and the contribution of prestin to echolocation has not yet been resolved. Here we report the expression of the protein prestin in five species of echolocating whales and two species of echolocating bats. Positive labeling in the basolateral membrane of outer hair cells, using three anti-prestin antibodies, was found all along the cochlear spiral in echolocating species. These findings provide morphological evidence that prestin can have a role in cochlear amplification in the basolateral membrane up to 120–180 kHz. In addition, labeling of the cochlea with a combination of anti-prestin, anti-neurofilament, anti-myosin VI and/or phalloidin and DAPI will be useful for detecting potential recent cases of noise-induced hearing loss in stranded cetaceans. This study improves our understanding of the mechanisms involved in sound transduction in echolocating mammals, as well as describing an optimized methodology for detecting cases of hearing loss in stranded marine mammals.
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spelling pubmed-73964972020-08-25 Echolocating Whales and Bats Express the Motor Protein Prestin in the Inner Ear: A Potential Marker for Hearing Loss Morell, Maria Vogl, A. Wayne IJsseldijk, Lonneke L. Piscitelli-Doshkov, Marina Tong, Ling Ostertag, Sonja Ferreira, Marisa Fraija-Fernandez, Natalia Colegrove, Kathleen M. Puel, Jean-Luc Raverty, Stephen A. Shadwick, Robert E. Front Vet Sci Veterinary Science Prestin is an integral membrane motor protein located in outer hair cells of the mammalian cochlea. It is responsible for electromotility and required for cochlear amplification. Although prestin works in a cycle-by-cycle mode up to frequencies of at least 79 kHz, it is not known whether or not prestin is required for the extreme high frequencies used by echolocating species. Cetaceans are known to possess a prestin coding gene. However, the expression and distribution pattern of the protein in the cetacean cochlea has not been determined, and the contribution of prestin to echolocation has not yet been resolved. Here we report the expression of the protein prestin in five species of echolocating whales and two species of echolocating bats. Positive labeling in the basolateral membrane of outer hair cells, using three anti-prestin antibodies, was found all along the cochlear spiral in echolocating species. These findings provide morphological evidence that prestin can have a role in cochlear amplification in the basolateral membrane up to 120–180 kHz. In addition, labeling of the cochlea with a combination of anti-prestin, anti-neurofilament, anti-myosin VI and/or phalloidin and DAPI will be useful for detecting potential recent cases of noise-induced hearing loss in stranded cetaceans. This study improves our understanding of the mechanisms involved in sound transduction in echolocating mammals, as well as describing an optimized methodology for detecting cases of hearing loss in stranded marine mammals. Frontiers Media S.A. 2020-07-17 /pmc/articles/PMC7396497/ /pubmed/32851016 http://dx.doi.org/10.3389/fvets.2020.00429 Text en Copyright © 2020 Morell, Vogl, IJsseldijk, Piscitelli-Doshkov, Tong, Ostertag, Ferreira, Fraija-Fernandez, Colegrove, Puel, Raverty and Shadwick. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Morell, Maria
Vogl, A. Wayne
IJsseldijk, Lonneke L.
Piscitelli-Doshkov, Marina
Tong, Ling
Ostertag, Sonja
Ferreira, Marisa
Fraija-Fernandez, Natalia
Colegrove, Kathleen M.
Puel, Jean-Luc
Raverty, Stephen A.
Shadwick, Robert E.
Echolocating Whales and Bats Express the Motor Protein Prestin in the Inner Ear: A Potential Marker for Hearing Loss
title Echolocating Whales and Bats Express the Motor Protein Prestin in the Inner Ear: A Potential Marker for Hearing Loss
title_full Echolocating Whales and Bats Express the Motor Protein Prestin in the Inner Ear: A Potential Marker for Hearing Loss
title_fullStr Echolocating Whales and Bats Express the Motor Protein Prestin in the Inner Ear: A Potential Marker for Hearing Loss
title_full_unstemmed Echolocating Whales and Bats Express the Motor Protein Prestin in the Inner Ear: A Potential Marker for Hearing Loss
title_short Echolocating Whales and Bats Express the Motor Protein Prestin in the Inner Ear: A Potential Marker for Hearing Loss
title_sort echolocating whales and bats express the motor protein prestin in the inner ear: a potential marker for hearing loss
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7396497/
https://www.ncbi.nlm.nih.gov/pubmed/32851016
http://dx.doi.org/10.3389/fvets.2020.00429
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